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Research Assistant IV (Cincinnati, OH): Plan & independently execute laboratory experiments related to acute myeloid leukemia models & normal human hematopoietic cells. Requires: Master’s degree in Bio or any subspecialty such as biochem, immun, med, mol bio, dev bio. 2 yrs exp which must have included: Lenti & Retro Virus Production, Collection & Concentration; Transfection; Flow cytometry & Analysis; Isolation, purification & culture of CD 34+ Hematopoietic Stem Cells (HSC's) from Human Umbilical Cord Blood; Methylcellulose Culture of Cells for Colony Formation Assays; Designing & Performing WST-1 Cell Proliferation Assays; Transcription analysis, protein expression using heterologous cell lines; Molecular Cloning; Immunohistochemistry & Immunocytochemistry; Laser Capture Microdissection; Lung Lavage, Inflation fixation and sectioning of lungs; Proteomics & Western Blot Analysis; Polymerase Chain Reaction, Real-Time PCR, RT-QPCR; RNA & DNA manipulation, site-directed mutagenesis & their functional applications; Transcriptional regulation, promoter-reporter analysis; Gel Electrophoresis & Spectrophotometry; Extensive Animal Handling experience including intravenous, intraperitoneal & subcutaneous injection, necropsy, Alzet pump implantation, ossicle implantation; Performing polyacrylamide gel electrophoresis (PAGE) analyses on cell culture and tissue samples; Performing Bradford assays; Developing & running enzyme-linked immunosorbent assays (ELISAs) in direct and sandwich formats; Preparing, loading & running agarose gel electrophoresis; Using instruments such as a centrifuge, pH meter, analytical balance, microplate reader, spectrophotometer, & LI-COR Odyssey Imager, IVIS Imaging Systems; Designing &analyzing Primers using Mac Vector software and Vector NTI; & Data analysis using GRAPHPAD Prism Software. Send resume & cover letter referencing Job #0565374 to James.Mulloy@cchmc.org. Cincinnati Children’s Hospital Medical Center is an Affirmative Action/Equal Opportunity Institution. Women and minorities are encouraged to apply.
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