Experimental Hematology and Cancer Biology

Division Details

Division Data Summary

Research and Training Details

Number of Faculty22
Number of Joint Appointment Faculty14
Number of Research Fellows33
Number of Research Students15
Number of Support Personnel89
Direct Annual Grant Support$8,138,423
Direct Annual Industry Support$121,107
Peer Reviewed Publications90

Division Photo

Group photo of the Division of Experimental Hemotology & Cancer Biology

Row 1: N Ratner, Y Zheng, J Degen

Row 2: D Pan, P Malik, MD Filippi, R Drissi, R Waclaw, A Kumar

Row 3: T Kalfa, R Meetei, S Wells, J Wu, J Mulloy, L Chow

Row 4: M Azam, F Guo, B DasGupta, N Nassar, G Huang

Row 5: D Starczynowski, M Flick, J Cancelas, P Andreassen, Q Pang, L Grimes

Division Highlights

Qishen Pang, PhD

Identification of leukemia-initiating cells (LICs) in FA AML patients and FA mice – We recently demonstrated that interleukin-3 receptor a (IL-3Ra) is a promising candidate as an LIC-specific antigen for FA AML. We are in the process of studying FA mouse LIC functionality using bone marrow transplantation assays.

Qishen Pang, PhD

Studies of the role of FA proteins in protecting anti-oxidant genes from oxidative damage. – We showed that certain important genes functioning in anti-oxidant defense and reactive oxygen species (ROS) metabolism were significantly downregulated in FA samples compared to those of normal donors. We then demonstrated a novel role for the FA pathway in cellular antioxidant defense.

Qishen Pang, PhD

Studies of the natural antioxidant Salidroside in HSC maintenance. – Using several mouse models deficient for DNA repair pathways (including the FA pathway) known to be involved in oxidative DNA damage repair, we demonstrate that Salidroside protects quiescent HSPCs from oxidative stress-induced cycling through stimulation the activity of poly(ADP-ribose)polymerase-1 (PARP-1), a component of the base excision repair pathway.

Nancy Ratner, PhD

Hennigan et al. showed that the NF2 tumor suppressor regulates microtubule –based vesicle trafficking via the novel Rac, MLK and p38SAPK pathway.

Yi Zheng, PhD

We have published a series of studies related to rational design, screening and validation of lead inhibitors targeting RhoA GTPase and NOX2 enzymes, defining the pathologic roles of GTPases RhoA, Rac1 and Cdc42 in cancer and blood diseases, and revealing the essential signaling pathways of Rho GTPases in neural, eye, heart, and blood development.

Jose Cancelas, MD, PhD

Our research focus is on intrinsic and extrinsic (microenvironment) signals controlling stem cell function in hematopoietic tissues and in leukemia. Specific projects include: Rac GTPases inhibition in chronic myelogenous leukemia, Vav / Rac as a molecular target in pediatric acute lymphoblastic leukemia, and connexin-43 in bone marrow failure after cancer-related chemotherapy.

Jianqiang Wu

We have made progress in understanding the molecular mechanisms of Neurofibroma tumorigenesis under neurofibromatosis type 1, and established a preclinical therapeutic testing of neurofibroma in mouse.

Dao Pan

With collaboration with Dr. Clinton Joiner's group under NIH Sickle Cell Center, we co-published our study on K-Cl cotransporter gene expression during human and murine erythroid differentiation on JBC. Our study on the application of secreted Gaussia luciferase (Gluc) as a marker for in vivo bioluminescent monitoring of system protein delivery, as well as its natural biodistribution in mice has been published on Molecular Biotechnology.

Paul Andreassen

The Andreassen lab has discovered that a ubiquitin-dependent signaling pathway, involving the RNF8 E3 ubiquitin ligase and the RAP80 ubiquitin-binding protein, recruits the core machinery for homologous recombination to sites of DNA damage through PALB2.

Marie-Dominique Filippi

The overarching goal of the research program of Dr Filippi’s lab is to understand the molecular regulation of hematopoietic cell functions. Specifically, we have been investigating the role of cell shape and cytoskeleton reorganization in modulating hematopoietic stem cell self renewal and engraftment, and neutrophil migration and trafficking. To do so, we are using genetics knock out animal models of regulators of cytoskeleton, namely Rho GTPases, and state of the art microscopy techniques, including live cell imaging, and immunofluorescence microscopy and multispectral imaging flow cytometry (Amnis ImageStream). Recent major findings from our work is the identification of a new role for p190-B-RhoGAP as a regulator of hematopoietic stem cell self renewal and cell fate decision during cell division. Furthermore, we are now showing that p190-B does so by regulating cell shape and polarity that ultimately influences the balance of asymmetric/symmetric self renewal. Other research project is to dissect the process of cell migration in neutrophils. We have made majors contribution to this field. Notably, we recently showed that Cdc42 unexpectedly uses aMb2 integrin signaling for efficient directed migration. A further understanding of the mechanism underlying these functions may lead to novel protocol of stem cell expansion ex vivo and novel therapeutic approach to neutrophilic inflammation, respectively.

Fukun Guo

We have made progress in studying the role of mTOR in stem  cell/ progenitor cell differentiation, and in defining the role of Cdc42 and RhoA GTPases in T cell activation.

James Mulloy

Dissection of the molecular pathogenesis of MLL-fusion AML and AML1-ETO-associated AML. Showed the importance of the Rac/Bcl family of proteins in MLL-fusion AML and the possibility of targeting these proteins therapeutically. Defined the role that Thrombopoeitic/MPL/Bcl-xL plays downstream of the AML1-ETO oncogene.

Daniel Starczynowski

We performed an shRNA screen to identify modifiers of Lenalidomide, characterized movel TRAF6 transgenic knockout and overexpression mice, characterized a novel TIFAB knockout mouse, developed a novel xenograft model using MDS-derived patient cells.  A research paper accepted on novel mechanisms of Bortezomib, and another research paper is being prepared for submission on targeting IRAK1 in MDS.

Carolyn Lutzko

I have made progress in developing gene therapy for patients with genetic diseases that are treatable through hematopoietic stem cell based therapies, developing iPSC lines from patient specific induced pluripotent stem cells to study the cell physiology of the disease and develop therapies, and designing new cell therapies for disease. 

Ronald Waclaw

We are currently writing two manuscripts: One describing the effect of Shp2 (PTPN11) mutations on brain development, specifically in the development of myelinating oligodendrocytes. These findings are significant because Shp2 is mutated in the RAS related disorders, Noonan and LEOPARD syndrome.  Patients in both of these syndromes exhibit neurocognitive defects.  We hope to understand the neurodevelopmental abnormalities that occur when Shp2 mutations are expressed and that this will provide evidence towards the developmental basis of the behavioral phenotypes. The other manuscript is identifying the role of the Zic genes, which are zinc-finger transcription factors, in the forebrain.  We have identified that a mouse model of Dandy-Walker syndrome (Zic1/4+/-) exhibits midline forebrain defects.  This is significant because only cerebellar defects have been described in previous studies with this mouse.   

Elke Grassman

My Lab has been awarded 2 large new contracts for work supporting gene transfer trials. This new work allows us to hire more staff and expand our services.

Johannes C.M. van der Loo

Contract manufacturing of research-grade and clinical grade viral vectors based on a fee-for-service model to support investigators locally, nationally and internationally with materials to support their research and phase I/II clinical trials.

Ruhikanta Meetei

We report the isolation and characterization of a novel 20-kDa FANCA-associated protein (FAAP20). We show that FAAP20 is an integral component of the FA nuclear core complex. We identify a region on FANCA that physically interacts with FAAP20, and show that FANCA regulates stability of this protein. FAAP20 contains a conserved ubiquitin-binding zinc-finger domain (UBZ), and binds K-63-linked ubiquitin chains in vitro. The FAAP20-UBZ domain is not required for interaction with FANCA, but is required for DNA-damage-induced chromatin loading of FANCA and the functional integrity of the FA pathway. These findings reveal critical roles for FAAP20 in the FA-BRCA pathway of DNA damage repair and genome maintenance.

Kakajan Komurov

We are interested in computational and experimental analyses of global molecular networks supporting tumorigenesis. Projects in the lab include development of novel computational tools and their use in integrated analyses of drug resistance networks alongside with experimentation.

Mathew Flick

Continued analysis of the pathogenesis of inflammatory arthritis including a publication highlighting the use of a novel targeting-agent with potential efficacy for diagnostic imaging and drug delivery.  A new project has been initiated, funded by a Cincinnati Children's Hospital Medical Center DHC pilot and feasibility grant, to study the mechanisms by which coagulation factors drive the pathogenesis of fatty liver disease.

Jay Degen

Dr. J. L. Degen presented a “State-of-the-Art” lecture on Hemostatic Factors in Cancer at the American Society of Hematology (ASH) Conference in San Diego, December 10, 2011, as well as presented the Simon Karpatkin Memorial Lecture at the 6th International Conference on Thrombosis and Hemostasis Issues in Cancer, in Bergamo, Italy, April 20, 2012.  In collaborative studies with investigators at the Rockefeller University, Dr. J. L. Degen’s laboratory has developed new insights into the cellular sources and biological importance of TGF-b1, a cytokine known to control cell proliferation, differentiation, immune cell function and thromboinflammatory disease process. Detailed studies reported in Blood  of  mice engineered to specifically lack platelet-derived TGF-b1 revealed that platelets contain the vast majority (>95%) of circulating TGF-b1, but the loss of platelet TGF-b1 does not alter hemostatic function in vivo.  However, mice lacking platelet-derived TGF-b1 were found to be protected from the development of cardiac hypertrophy, fibrosis, and systolic dysfunction following a pressure overload challenge. Together with Dr. Punam Malik, interventions at the level of platelet-derived TGF-b1 are currently being explored as means of limiting cardiovascular pathologies associated with sickle cell disease.

Nicolas Nassar

My research focuses on understanding the structure/function relationship of signaling proteins involved in cancer propagation and initiation and on finding ways to inhibit them by targeted rational drug design. More specifically, we are targeting oncogenic Ras in cancer.

Theodosia Kalfa

The Kalfa lab had a significant publication in Blood demonstrating that erythroblast enucleation is a more complex process than previously thought requiring a multistep action of tubulin and filamentous actin, as well as lipid raft formation coordinated by Rac GTPases.

Lionel Chow

The Chow lab is studying a form of aggressive brain tumor called high-grade glioma.  Using novel mouse models for this disease, we are investigating the molecular characteristics of different tumor subgroups as well as distinguishing features of invasive disease, which is responsible for treatment failure and patient mortality.  We are also using these models to develop novel therapeutic approaches for this disease.

Benjamin Mizukawa

Our work demonstrating that Rac GTPase survival signaling through Bcl-2 proteins may be therapeutically targeted in MLL fusion-mediated acute myeloid leukemia was published in the journal Blood.

Janos Sumegi

Gene expression analysis of primary and secondary hemophagocytic lymphohistiocytosis.  Molecular analysis of fusion oncogenes and their products in pediatric soft tissue sarcomas.

Punam Malik

We will be starting the clinical trial for gene therapy for Sickle Cell Disease.  We have also began working on gene therapy for HLH with Drs. Jordan and Risma.  A clinical trial for Sickle Nephropathy has begun.

Division Publications

  1. Akbar H, Shang X, Perveen R, Berryman M, Funk K, Johnson JF, Tandon NN, Zheng Y. Gene targeting implicates Cdc42 GTPase in GPVI and non-GPVI mediated platelet filopodia formation, secretion and aggregation. PLoS One. 2011; 6:e22117.
  2. Ali AM, Pradhan A, Singh TR, Du C, Li J, Wahengbam K, Grassman E, Auerbach AD, Pang Q, Meetei AR. FAAP20: a novel ubiquitin-binding FA nuclear core complex protein required for functional integrity of the FA-BRCA DNA repair pathway. Blood. 2012; 119:3285-94.
  3. Bindels EM, Havermans M, Lugthart S, Erpelinck C, Wocjtowicz E, Krivtsov AV, Rombouts E, Armstrong SA, Taskesen E, Haanstra JR, Beverloo HB, Dohner H, Hudson WA, Kersey JH, Delwel R, Kumar AR. EVI1 is critical for the pathogenesis of a subset of MLL-AF9-rearranged AMLs. Blood. 2012; 119:5838-49.
  4. Bosco EE, Kumar S, Marchioni F, Biesiada J, Kordos M, Szczur K, Meller J, Seibel W, Mizrahi A, Pick E, Filippi MD, Zheng Y. Rational design of small molecule inhibitors targeting the Rac GTPase-p67(phox) signaling axis in inflammation. Chem Biol. 2012; 19:228-42.
  5. Cancelas JA. Adhesion, migration, and homing of murine hematopoietic stem cells and progenitors. Methods Mol Biol. 2011; 750:187-96.
  6. Cancelas JA. On how Rac controls hematopoietic stem cell activity. Transfusion. 2011; 51 Suppl 4:153S-159S.
  7. Cancelas JA, Rugg N, Fletcher D, Pratt PG, Worsham DN, Dunn SK, Marschner S, Reddy HL, Goodrich RP. In vivo viability of stored red blood cells derived from riboflavin plus ultraviolet light-treated whole blood. Transfusion. 2011; 51:1460-8.
  8. Cancelas JA, Rugg N, Pratt PG, Worsham DN, Pehta JC, Banks K, Davenport RD, Judd WJ. Infusion of P-Capt prion-filtered red blood cell products demonstrate acceptable in vivo viability and no evidence of neoantigen formation. Transfusion. 2011; 51:2228-36.
  9. Chauhan BK, Lou M, Zheng Y, Lang RA. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proc Natl Acad Sci U S A. 2011; 108:18289-94.
  10. Chernoguz A, Crawford K, Donovan E, Vandersall A, Berglund C, Cripe TP, Frischer JS. EGFR inhibition fails to suppress vascular proliferation and tumor growth in a Ewing's sarcoma model. J Surg Res. 2012; 173:1-9.
  11. Chow LM, Baker SJ. Capturing the molecular and biological diversity of high-grade astrocytoma in genetically engineered mouse models. Oncotarget. 2012; 3:67-77.
  12. Degen JL, Palumbo JS. Hemostatic factors, innate immunity and malignancy. Thromb Res. 2012; 129 Suppl 1:S1-5.
  13. Dietz DM, Sun H, Lobo MK, Cahill ME, Chadwick B, Gao V, Koo JW, Mazei-Robison MS, Dias C, Maze I, Damez-Werno D, Dietz KC, Scobie KN, Ferguson D, Christoffel D, Ohnishi Y, Hodes GE, Zheng Y, Neve RL, Hahn KM, Russo SJ, Nestler EJ. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci. 2012; 15:891-896.
  14. Dorris K, Fouladi M, Davies SM, Perentesis JP, Lawrence JM, Chow LM, Assa'ad A, Uygungil B, Jodele S. Severe allergic reactions to thiol-based cytoprotective agents mesna and amifostine in a child with a supratentorial primitive neuroectodermal tumor. J Pediatr Hematol Oncol. 2011; 33:e250-2.
  15. Drissi R, Wu J, Hu Y, Bockhold C, Dome JS. Telomere shortening alters the kinetics of the DNA damage response after ionizing radiation in human cells. Cancer Prev Res (Phila). 2011; 4:1973-81.
  16. Du W, Rani R, Sipple J, Schick J, Myers KC, Mehta P, Andreassen PR, Davies SM, Pang Q. The FA pathway counteracts oxidative stress through selective protection of antioxidant defense gene promoters. Blood. 2012; 119:4142-51.
  17. Elmeliegy MA, Carcaboso AM, LM LC, Zhang ZM, Calabrese C, Throm SL, Wang F, Baker SJ, Stewart CF. Magnetic resonance imaging-guided microdialysis cannula implantation in a spontaneous high-grade glioma murine model. J Pharm Sci. 2011; 100:4210-4214.
  18. Fang J, Varney M, Starczynowski DT. Implication of microRNAs in the Pathogenesis of MDS. Curr Pharm Des. 2012; 18:3170-9.
  19. Florian MC, Dorr K, Niebel A, Daria D, Schrezenmeier H, Rojewski M, Filippi MD, Hasenberg A, Gunzer M, Scharffetter-Kochanek K, Zheng Y, Geiger H. Cdc42 activity regulates hematopoietic stem cell aging and rejuvenation. Cell Stem Cell. 2012; 10:520-30.
  20. Geh E, Meng Q, Mongan M, Wang J, Takatori A, Zheng Y, Puga A, Lang RA, Xia Y. Mitogen-activated protein kinase kinase kinase 1 (MAP3K1) integrates developmental signals for eyelid closure. Proc Natl Acad Sci U S A. 2011; 108:17349-54.
  21. Geiger H, Pawar SA, Kerschen EJ, Nattamai KJ, Hernandez I, Liang HP, Fernandez JA, Cancelas JA, Ryan MA, Kustikova O, Schambach A, Fu Q, Wang J, Fink LM, Petersen KU, Zhou D, Griffin JH, Baum C, Weiler H, Hauer-Jensen M. Pharmacological targeting of the thrombomodulin-activated protein C pathway mitigates radiation toxicity. Nat Med. 2012; 18:1123-1129.
  22. George A, Benton J, Pratt J, Kim MO, Kalinyak KA, Kalfa TA, Joiner CH. The impact of the 2009 H1N1 influenza pandemic on pediatric patients with sickle cell disease. Pediatr Blood Cancer. 2011; 57:648-53.
  23. Goldsmith JC, Bonham VL, Joiner CH, Kato GJ, Noonan AS, Steinberg MH. Framing the research agenda for sickle cell trait: building on the current understanding of clinical events and their potential implications. Am J Hematol. 2012; 87:340-6.
  24. Goyama S, Mulloy JC. Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. Int J Hematol. 2011; 94:126-33.
  25. Hines J, Mitchell M, Crosby L, Johnson A, Valenzuela J, Kalinyak K, Joiner C. Engaging Patients With Sickle Cell Disease and Their Families in Disease Education, Research, and Community Awareness. Journal of Prevention & Intervention in the Community. 2011; 39:256-272.
  26. Horowitz NA, Blevins EA, Miller WM, Perry AR, Talmage KE, Mullins ES, Flick MJ, Queiroz KC, Shi K, Spek CA, Conway EM, Monia BP, Weiler H, Degen JL, Palumbo JS. Thrombomodulin is a determinant of metastasis through a mechanism linked to the thrombin binding domain but not the lectin-like domain. Blood. 2011; 118:2889-95.
  27. Horowitz NA, Palumbo JS. PL-25 Mechanisms coupling thrombomodulin to tumor dissemination. Thromb Res. 2012; 129 Suppl 1:S119-21.
  28. Hoskins EE, Morreale RJ, Werner SP, Higginbotham JM, Laimins LA, Lambert PF, Brown DR, Gillison ML, Nuovo GJ, Witte DP, Kim MO, Davies SM, Mehta PA, Kovacic MB, Wikenheiser-Brokamp KA, Wells SI. The Fanconi Anemia Pathway Limits Human Papillomavirus Replication. J Virol. 2012; 86:8131-8.
  29. Huang G, Zhao X, Wang L, Elf S, Xu H, Zhao X, Sashida G, Zhang Y, Liu Y, Lee J, Menendez S, Yang Y, Yan X, Zhang P, Tenen DG, Osato M, Hsieh JJ, Nimer SD. The ability of MLL to bind RUNX1 and methylate H3K4 at PU.1 regulatory regions is impaired by MDS/AML-associated RUNX1/AML1 mutations. Blood. 2011; 118:6544-52.
  30. Kaimal V, Chu Z, Mahller YY, Papahadjopoulos-Sternberg B, Cripe TP, Holland SK, Qi X. Saposin C Coupled Lipid Nanovesicles Enable Cancer-Selective Optical and Magnetic Resonance Imaging. Mol Imaging Biol. 2011; 13:886-897.
  31. Kalamarides M, Acosta MT, Babovic-Vuksanovic D, Carpen O, Cichowski K, Evans DG, Giancotti F, Hanemann CO, Ingram D, Lloyd AC, Mayes DA, Messiaen L, Morrison H, North K, Packer R, Pan D, Stemmer-Rachamimov A, Upadhyaya M, Viskochil D, Wallace MR, Hunter-Schaedle K, Ratner N. Neurofibromatosis 2011: a report of the Children's Tumor Foundation annual meeting. Acta Neuropathol. 2012; 123:369-80.
  32. Kavanaugh GM, Wise-Draper TM, Morreale RJ, Morrison MA, Gole B, Schwemberger S, Tichy ED, Lu L, Babcock GF, Wells JM, Drissi R, Bissler JJ, Stambrook PJ, Andreassen PR, Wiesmuller L, Wells SI. The human DEK oncogene regulates DNA damage response signaling and repair. Nucleic Acids Res. 2011; 39:7465-76.
  33. Kohler A, Geiger H, Gunzer M. Imaging hematopoietic stem cells in the marrow of long bones in vivo. Methods Mol Biol. 2011; 750:215-24.
  34. Komurov K. Modeling community-wide molecular networks of multicellular systems. Bioinformatics. 2012; 28:694-700.
  35. Konstantinidis DG, Pushkaran S, Johnson JF, Cancelas JA, Manganaris S, Harris CE, Williams DA, Zheng Y, Kalfa TA. Signaling and cytoskeletal requirements in erythroblast enucleation. Blood. 2012; 119:6118-27.
  36. Kuchenbauer F, Mah SM, Heuser M, McPherson A, Ruschmann J, Rouhi A, Berg T, Bullinger L, Argiropoulos B, Morin RD, Lai D, Starczynowski DT, Karsan A, Eaves CJ, Watahiki A, Wang Y, Aparicio SA, Ganser A, Krauter J, Dohner H, Dohner K, Marra MA, Camargo FD, Palmqvist L, Buske C, Humphries RK. Comprehensive analysis of mammalian miRNA* species and their role in myeloid cells. Blood. 2011; 118:3350-8.
  37. Leslie JR, Imai F, Zhou X, Lang RA, Zheng Y, Yoshida Y. RhoA is dispensable for axon guidance of sensory neurons in the mouse dorsal root ganglia. Front Mol Neurosci. 2012; 5:67.
  38. Levantini E, Lee S, Radomska HS, Hetherington CJ, Alberich-Jorda M, Amabile G, Zhang P, Gonzalez DA, Zhang J, Basseres DS, Wilson NK, Koschmieder S, Huang G, Zhang DE, Ebralidze AK, Bonifer C, Okuno Y, Gottgens B, Tenen DG. RUNX1 regulates the CD34 gene in haematopoietic stem cells by mediating interactions with a distal regulatory element. EMBO J. 2011; 30:4059-70.
  39. Li X, Sipple J, Pang Q, Du W. Salidroside stimulates DNA repair enzyme Parp-1 activity in mouse HSC maintenance. Blood. 2012; 119:4162-73.
  40. Li Z, Huang H, Chen P, He M, Li Y, Arnovitz S, Jiang X, He C, Hyjek E, Zhang J, Zhang Z, Elkahloun A, Cao D, Shen C, Wunderlich M, Wang Y, Neilly MB, Jin J, Wei M, Lu J, Valk PJ, Delwel R, Lowenberg B, Le Beau MM, Vardiman J, Mulloy JC, Zeleznik-Le NJ, Liu PP, Zhang J, Chen J. miR-196b directly targets both HOXA9/MEIS1 oncogenes and FAS tumour suppressor in MLL-rearranged leukaemia. Nat Commun. 2012; 3:688.
  41. Liu M, Bi F, Zhou X, Zheng Y. Rho GTPase regulation by miRNAs and covalent modifications. Trends Cell Biol. 2012; 22:365-73.
  42. Liu M, Tang Q, Qiu M, Lang N, Li M, Zheng Y, Bi F. miR-21 targets the tumor suppressor RhoB and regulates proliferation, invasion and apoptosis in colorectal cancer cells. FEBS Lett. 2011; 585:2998-3005.
  43. Liu W, Feng Y, Shang X, Zheng Y. Rho GTPases in hematopoietic stem/progenitor cell migration. Methods Mol Biol. 2011; 750:307-19.
  44. Maddala R, Chauhan BK, Walker C, Zheng Y, Robinson ML, Lang RA, Rao PV. Rac1 GTPase-deficient mouse lens exhibits defects in shape, suture formation, fiber cell migration and survival. Dev Biol. 2011; 360:30-43.
  45. Maiques-Diaz A, Chou FS, Wunderlich M, Gomez-Lopez G, Jacinto FV, Rodriguez-Perales S, Larrayoz MJ, Calasanz MJ, Mulloy JC, Cigudosa JC, Alvarez S. Chromatin modifications induced by the AML1-ETO fusion protein reversibly silence its genomic targets through AML1 and Sp1 binding motifs. Leukemia. 2012; 26:1329-37.
  46. Mayes DA, Rizvi TA, Cancelas JA, Kolasinski NT, Ciraolo GM, Stemmer-Rachamimov AO, Ratner N. Perinatal or adult Nf1 inactivation using tamoxifen-inducible PlpCre each cause neurofibroma formation. Cancer Res. 2011; 71:4675-85.
  47. Meyer A, Wang W, Qu J, Croft L, Degen JL, Coller BS, Ahamed J. Platelet TGF-beta1 contributions to plasma TGF-beta1, cardiac fibrosis, and systolic dysfunction in a mouse model of pressure overload. Blood. 2012; 119:1064-74.
  48. Mizukawa B, Wei J, Shrestha M, Wunderlich M, Chou FS, Griesinger A, Harris CE, Kumar AR, Zheng Y, Williams DA, Mulloy JC. Inhibition of Rac GTPase signaling and downstream prosurvival Bcl-2 proteins as combination targeted therapy in MLL-AF9 leukemia. Blood. 2011; 118:5235-45.
  49. Montes de Oca R, Andreassen PR, Wilson KL. Barrier-to-Autointegration Factor influences specific histone modifications. Nucleus. 2011; 2:580-90.
  50. Morrison MA, Morreale RJ, Akunuru S, Kofron M, Zheng Y, Wells SI. Targeting the human papillomavirus E6 and E7 oncogenes through expression of the bovine papillomavirus type 1 E2 protein stimulates cellular motility. J Virol. 2011; 85:10487-98.
  51. Muller LU, Milsom MD, Harris CE, Vyas R, Brumme KM, Parmar K, Moreau LA, Schambach A, Park IH, London WB, Strait K, Schlaeger T, Devine AL, Grassman E, D'Andrea A, Daley GQ, Williams DA. Overcoming reprogramming resistance of Fanconi anemia cells. Blood. 2012; 119:5449-57.
  52. Myers KC, Bleesing JJ, Davies SM, Zhang X, Martin LJ, Mueller R, Harris RE, Filipovich AH, Kovacic MB, Wells SI, Mehta PA. Impaired immune function in children with Fanconi anaemia. Br J Haematol. 2011; 154:234-40.
  53. Navarro-Lerida I, Sanchez-Perales S, Calvo M, Rentero C, Zheng Y, Enrich C, Del Pozo MA. A palmitoylation switch mechanism regulates Rac1 function and membrane organization. EMBO J. 2012; 31:534-51.
  54. Nawrot M, McKenna DH, Sumstad D, McMannis JD, Szczepiorkowski ZM, Belfield H, Grassman E, Temples T, Nielsen D, Yuan N, Wognum B, Reems JA. Interlaboratory assessment of a novel colony-forming unit assay: a multicenter study by the cellular team of Biomedical Excellence for Safer Transfusion (BEST) collaborative. Transfusion. 2011; 51:2001-5.
  55. Negrotto S, Ng KP, Jankowska AM, Bodo J, Gopalan B, Guinta K, Mulloy JC, Hsi E, Maciejewski J, Saunthararajah Y. CpG methylation patterns and decitabine treatment response in acute myeloid leukemia cells and normal hematopoietic precursors. Leukemia. 2012; 26:244-54.
  56. Ng KP, Ebrahem Q, Negrotto S, Mahfouz RZ, Link KA, Hu Z, Gu X, Advani A, Kalaycio M, Sobecks R, Sekeres M, Copelan E, Radivoyevitch T, Maciejewski J, Mulloy JC, Saunthararajah Y. p53 independent epigenetic-differentiation treatment in xenotransplant models of acute myeloid leukemia. Leukemia. 2011; 25:1739-50.
  57. Pan D, Kalfa TA, Wang D, Risinger M, Crable S, Ottlinger A, Chandra S, Mount DB, Hubner CA, Franco RS, Joiner CH. K-Cl Cotransporter Gene Expression during Human and Murine Erythroid Differentiation. J Biol Chem. 2011; 286:30492-503.
  58. Plageman TF, Jr., Chauhan BK, Yang C, Jaudon F, Shang X, Zheng Y, Lou M, Debant A, Hildebrand JD, Lang RA. A Trio-RhoA-Shroom3 pathway is required for apical constriction and epithelial invagination. Development. 2011; 138:5177-88.
  59. Qi X, Flick MJ, Frederick M, Chu Z, Mason R, DeLay M, Thornton S. Saposin C coupled lipid nanovesicles specifically target arthritic mouse joints for optical imaging of disease severity. PLoS One. 2012; 7:e33966.
  60. Quarmyne MO, Risinger M, Linkugel A, Frazier A, Joiner C. Volume regulation and KCl cotransport in reticulocyte populations of sickle and normal red blood cells. Blood Cells Mol Dis. 2011; 47:95-9.
  61. Raghu H, Flick MJ. Targeting the coagulation factor fibrinogen for arthritis therapy. Curr Pharm Biotechnol. 2011; 12:1497-506.
  62. Rhyasen GW, Starczynowski DT. Deregulation of microRNAs in myelodysplastic syndrome. Leukemia. 2012; 26:13-22.
  63. Sashida G, Bae N, Di Giandomenico S, Asai T, Gurvich N, Bazzoli E, Liu Y, Huang G, Zhao X, Menendez S, Nimer SD. The mef/elf4 transcription factor fine tunes the DNA damage response. Cancer Res. 2011; 71:4857-65.
  64. Sengupta A, Cancelas JA. A conundrum in mammalian hematopoietic stem cell polarity. Cell Cycle. 2011; 10:4191-2.
  65. Sengupta A, Ficker AM, Dunn SK, Madhu M, Cancelas JA. Bmi1 reprograms CML B-lymphoid progenitors to become B-ALL-initiating cells. Blood. 2012; 119:494-502.
  66. Shang X, Cancelas JA, Li L, Guo F, Liu W, Johnson JF, Ficker A, Daria D, Geiger H, Ratner N, Zheng Y. R-Ras and Rac GTPase cross-talk regulates hematopoietic progenitor cell migration, homing, and mobilization. J Biol Chem. 2011; 286:24068-78.
  67. Shang X, Marchioni F, Sipes N, Evelyn CR, Jerabek-Willemsen M, Duhr S, Seibel W, Wortman M, Zheng Y. Rational Design of Small Molecule Inhibitors Targeting RhoA Subfamily Rho GTPases. Chem Biol. 2012; 19:699-710.
  68. Singbartl K, Bishop JV, Wen X, Murugan R, Chandra S, Filippi MD, Kellum JA. Differential effects of kidney-lung cross-talk during acute kidney injury and bacterial pneumonia. Kidney Int. 2011; 80:633-44.
  69. Sipes NS, Feng Y, Guo F, Lee HO, Chou FS, Cheng J, Mulloy J, Zheng Y. Cdc42 regulates extracellular matrix remodeling in three dimensions. J Biol Chem. 2011; 286:36469-77.
  70. Sorensen I, Susnik N, Inhester T, Degen JL, Melk A, Haller H, Schmitt R. Fibrinogen, acting as a mitogen for tubulointerstitial fibroblasts, promotes renal fibrosis. Kidney Int. 2011; 80:1035-44.
  71. Starczynowski DT, Lockwood WW, Delehouzee S, Chari R, Wegrzyn J, Fuller M, Tsao MS, Lam S, Gazdar AF, Lam WL, Karsan A. TRAF6 is an amplified oncogene bridging the RAS and NF-kappaB pathways in human lung cancer. J Clin Invest. 2011; 121:4095-105.
  72. Stengel K, Zheng Y. Cdc42 in oncogenic transformation, invasion, and tumorigenesis. Cell Signal. 2011; 23:1415-23.
  73. Stengel KR, Zheng Y. Essential role of cdc42 in ras-induced transformation revealed by gene targeting. PLoS One. 2012; 7:e37317.
  74. Sullivan BP, Kassel KM, Jone A, Flick MJ, Luyendyk JP. Fibrin(ogen)-Independent Role of Plasminogen Activators in Acetaminophen-Induced Liver Injury. Am J Pathol. 2012; 180:2321-9.
  75. Sundaram N, Bennett M, Wilhelm J, Kim MO, Atweh G, Devarajan P, Malik P. Biomarkers for early detection of sickle nephropathy. Am J Hematol. 2011; 86:559-66.
  76. Tan Z, Wortman M, Dillehay KL, Seibel WL, Evelyn CR, Smith SJ, Malkas LH, Zheng Y, Lu S, Dong Z. Small Molecule Targeting of PCNA Chromatin Association Inhibits Tumor Cell Growth. Mol Pharmacol. 2012; 81:811-9.
  77. Taniguchi Ishikawa E, Gonzalez-Nieto D, Ghiaur G, Dunn SK, Ficker AM, Murali B, Madhu M, Gutstein DE, Fishman GI, Barrio LC, Cancelas JA. Connexin-43 prevents hematopoietic stem cell senescence through transfer of reactive oxygen species to bone marrow stromal cells. Proc Natl Acad Sci U S A. 2012; 109:9071-6.
  78. van der Loo JC, Swaney WP, Grassman E, Terwilliger A, Higashimoto T, Schambach A, Baum C, Thrasher AJ, Williams DA, Nordling DL, Reeves L, Malik P. Scale-up and manufacturing of clinical-grade self-inactivating gamma-retroviral vectors by transient transfection. Gene Ther. 2012; 19:246-54.
  79. Vidal B, Ardite E, Suelves M, Ruiz-Bonilla V, Janue A, Flick MJ, Degen JL, Serrano AL, Munoz-Canoves P. Amelioration of Duchenne muscular dystrophy in mdx mice by elimination of matrix-associated fibrin-driven inflammation coupled to the alphaMbeta2 leukocyte integrin receptor. Hum Mol Genet. 2012; 21:1989-2004.
  80. Wang J, Geiger H, Rudolph KL. Immunoaging induced by hematopoietic stem cell aging. Curr Opin Immunol. 2011; 23:532-6.
  81. Wang L, Gural A, Sun XJ, Zhao X, Perna F, Huang G, Hatlen MA, Vu L, Liu F, Xu H, Asai T, Deblasio T, Menendez S, Voza F, Jiang Y, Cole PA, Zhang J, Melnick A, Roeder RG, Nimer SD. The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation. Science. 2011; 333:765-9.
  82. Wang PS, Wang J, Zheng Y, Pallen CJ. Loss of Protein-tyrosine Phosphatase alpha (PTP alpha) Increases Proliferation and Delays Maturation of Oligodendrocyte Progenitor Cells. J Biol Chem. 2012; 287:12529-40.
  83. Wood M, Rawe M, Johansson G, Pang S, Soderquist RS, Patel AV, Nelson S, Seibel W, Ratner N, Sanchez Y. Discovery of a Small Molecule Targeting IRA2 Deletion in Budding Yeast and Neurofibromin Loss in Malignant Peripheral Nerve Sheath Tumor Cells. Mol Cancer Ther. 2011; 10:1740-50.
  84. Wu J, Dombi E, Jousma E, Scott Dunn R, Lindquist D, Schnell BM, Kim MO, Kim A, Widemann BC, Cripe TP, Ratner N. Preclinical testing of sorafenib and RAD001 in the Nf(flox/flox) ;DhhCre mouse model of plexiform neurofibroma using magnetic resonance imaging. Pediatr Blood Cancer. 2012; 58:173-80.
  85. Xiang SY, Vanhoutte D, Del Re DP, Purcell NH, Ling H, Banerjee I, Bossuyt J, Lang RA, Zheng Y, Matkovich SJ, Miyamoto S, Molkentin JD, Dorn GW, 2nd, Brown JH. RhoA protects the mouse heart against ischemia/reperfusion injury. J Clin Invest. 2011; 121:3269-76.
  86. Yang J, Ylipaa A, Sun Y, Zheng H, Chen K, Nykter M, Trent J, Ratner N, Lev DC, Zhang W. Genomic and Molecular Characterization of Malignant Peripheral Nerve Sheath Tumor Identifies the IGF1R Pathway as a Primary Target for Treatment. Clin Cancer Res. 2011; 17:7563-7573.
  87. Yoshimi M, Goyama S, Kawazu M, Nakagawa M, Ichikawa M, Imai Y, Kumano K, Asai T, Mulloy JC, Kraft AS, Takahashi T, Shirafuji N, Kurokawa M. Multiple phosphorylation sites are important for RUNX1 activity in early hematopoiesis and T-cell differentiation. Eur J Immunol. 2012; 42:1044-50.
  88. You J, Wells SI. Human Papillomaviruses and Cancer. Cancer associated viruses. New York: Springer; 2011:463-487.
  89. Zhang S, Zhou X, Lang RA, Guo F. RhoA of the Rho family small GTPases is essential for B lymphocyte development. PLoS One. 2012; 7:e33773.
  90. Zuber J, Shi J, Wang E, Rappaport AR, Herrmann H, Sison EA, Magoon D, Qi J, Blatt K, Wunderlich M, Taylor MJ, Johns C, Chicas A, Mulloy JC, Kogan SC, Brown P, Valent P, Bradner JE, Lowe SW, Vakoc CR. RNAi screen identifies Brd4 as a therapeutic target in acute myeloid leukaemia. Nature. 2011; 478:524-8.

Faculty, Staff, and Trainees

Faculty Members

Yi Zheng, PhD, Professor
Leadership Division Director; Endowed Chair; Program Leader; Executive Co-Director, CBDI
Research Interests Signaling and Drug Discovery Program
Paul Andreassen, PhD, Assistant Professor
Research Interests Signaling and Drug Discovery Program
Jose Cancelas, MD, PhD, Associate Professor
Leadership Program Leader; Director, Flow Cytometry Core Facility
Research Interests Stem Cell Program
Jay Degen, PhD, Professor
Leadership Program Leader
Research Interests Hemostasis and Thrombosis Program
Marie-Dominique Filippi, PhD, Assistant Professor
Research Interests Stem Cell Program
Matthew Flick, PhD, Assistant Professor
Research Interests Hemostasis and Thrombosis Program
Elke Grassman, PhD, HCLD, Assistant Professor
Leadership Director, TTDSL
Research Interests Translational Core Laboratories
Fukun Guo, PhD, Assistant Professor
Research Interests Signaling and Drug Discovery Program
Kakajan Komurov, PhD, Assistant Professor
Research Interests Cancer Biology and Neural Tumors Program
Carolyn Lutzko, PhD, Associate Professor
Leadership Scientific Director of the Cell Processing and Manipulation Laboratory
Research Interests Translational Core Laboratories
Punam Malik, MD, Professor
Leadership Program Leader; Director of Translational Core Laboratory
Research Interests Hematology and Gene Therapy Program
Ruhikanta Meetei, PhD, Assistant Professor
Research Interests Signaling and Drug Discovery Program
Shyra Miller, PhD, Assistant Professor
Research Interests Cancer Biology and Neural Tumors Program
James Mulloy, PhD, Associate Professor
Leadership Program Leader
Research Interests Hematological Malignancy Program
Nicolas Nassar, PhD, Associate Professor
Research Interests Signaling and Drug Discovery Program
Dao Pan, PhD, Assistant Professor
Research Interests Hematology and Gene Therapy Program
Qishen Pang, PhD, Associate Professor
Research Interests Signaling and Drug Discovery Program
Nancy Ratner, PhD, Professor
Leadership Program Leader; Endowed Chair
Research Interests Cancer Biology and Neural Tumors Program
Daniel Starczynowski, PhD, Assistant Professor
Research Interests Hematological Malignancy Program
Johannes van der Loo, PhD, Associate Professor
Leadership Director, Aseptic Processing Laboratories; Director, Vector Production Facility; Chair, Institutional Biosafety Committee
Research Interests Translational Core Laboratories
Ronald Waclaw, , Assistant Professor
Research Interests Cancer Biology and Neural Tumors Program
Jianqiang Wu, MD, MS, Instructor
Research Interests Cancer Biology and Neural Tumors Program

Joint Appointment Faculty Members

Mohammed Azam, PhD, Assistant Professor (Cancer Pathology)
Research Interests Hematology Malignancy Program
Lionel Chow, MD, PhD, Assistant Professor (Oncology)
Research Interests Cancer Biology and Neural Tumors Program
Biplab DasGupta, PhD, Assistant Professor (Oncology)
Research Interests Cancer Biology and Neural Tumors Program
Rachid Drissi, PhD, Assistant Professor (Oncology)
Research Interests Cancer Biology and Neural Tumors Program
Hartmut Geiger, PhD, Associate Professor (Adjunct)
Research Interests Stem Cell Program
Leighton Grimes, PhD, Associate Professor (Immunobiology)
Research Interests Hematology Malignancy Program
Gang Huang, PhD, Assistant Professor (Cancer Pathology)
Research Interests Hematology Malignancy Program
Theodosia Kalfa, MD, PhD, Assistant Professor (Hematology)
Research Interests Hematology Malignancy Program
Ashish Kumar, MD, PhD, Assistant Professor (Bone Marrow Transplantation and Immune Deficiency)
Research Interests Hematology Malignancy program
Benjamin Mizukawa, MD, Instructor (Oncology)
Research Interests Hematological Malignancy Program
Eric Mullins, MD, Assistant Professor (Hematology)
Research Interests Hemostasis and Thrombosis Program
Joseph Palumbo, MD, Associate Professor (Hematology)
Research Interests Hemostasis and Thrombosis Program
Janos Sumegi, MD, PhD, Professor (Blood and Marrow Transplantation and Immune Deficiency)
Research Interests Hematology and Gene Therapy Program
Susanne Wells, PhD, Associate Professor (Oncology)
Research Interests Cancer Biology and Neural Tumors Program

Trainees

  • Shailaja Akunuru, PhD, 2011, University of Cincinnati
  • Gregory Bick, MS, 2010, University of Cincinnati
  • Gasilina Anjelika, , 2011
  • Kyung-Hee Chang, PhD, PGY-4, University of Florida
  • Wei Du, MD, PhD, 2007, Graduate School of Medicine, Tohoku University, Japan
  • Marthe-Sandrine Eiymo Mwa Mpollo, Msc, University of Toronto
  • Salim El-Amouri, PhD, 2010, Medical University of South Carolina
  • Chris Evelyn, PhD, 2009, University of Michigan-Ann Arbor
  • Yuxin Feng, PhD, 2007, BioChain Institute
  • Susuma Goyama, PhD, 2009, Graduate School of Medicine, University of Tokyo
  • Andrea Griesinger, MS, 2010, Colorado School of Mines
  • Li Guo, PhD, 2006, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China
  • Robert Hennigan,
  • Ashwini Hinge, PhD, PL-2, National Center for Cell Science, Pune University of Pune, Maharashtra, India
  • Novelle Kimmich, PhD, UC Irvine
  • Sachin Kumar, PhD, PL-2, Central Drug Research Institute (CDRI), Lucknow India
  • Leesa Sampson, PhD, 2010, Vanderbilt University
  • Jie Li, PhD, 2007, Academy of Sciences, China
  • Shan Lin, MS, 2010, Tsinghua University, Beijing China
  • Kevin Link, PhD, 2007, University of Cincinnati
  • Wei Liu, PhD, 2011
  • Debra Mayes, PhD, 2006, University of Arkansas for Medical Sciences
  • Rachel Oberst, BS, PGY-V, University of Louisville
  • Nicholas Olshavsky, PhD, 2010, University of Cincinnati
  • Jung-Young Park, PhD, 2010, National Institutes of Health
  • Ami V. Patel, PhD, 2009, University of Louisville
  • Deanna Patmore, BS, PGY-V, Voorhees College
  • Aran Pradhan, PhD, 2011, ICEGB, New Delhi, India
  • Joni Ullman Prasad, , PGY-V, Ohio State University
  • Garrett Rhyasen, BSc, PGY-2, University of Victoria, Canada
  • Amitava Sengupta, PhD, 2008, Jadavpur University/Saha Institute of Nuclear Physics Kolkata, India
  • Haley Titus-Mitchell, MS, PGY-II, Wright State University
  • Melinda Varney, PhD, PDF, Marshall University, WV
  • Shiv Viswanathan, PhD, 2003, University of Cincinnati
  • Inuk Zandvakili, MD, PhD, 2009, The University of Western Ontario
  • Shuangmin Zhang, PhD, PL-2, University of Texas
  • Xuan Zhou, PhD, 2008
  • Benjamin Mizukawa, MD, 2008, University of Utah School of Medicine
  • Thiyam Singh, PhD, 2004, CDRI Lucknow India
  • Abdullah Ali, PhD, 2005, IISC, Banglaore, India
  • Lisa Trump, PhD, PG1, University of Illinois
  • Jing Fang, PhD, Maine Medical Center
  • Junqi Yang, PhD, PL-1, University of Cincinnati
  • Mei Dai, PhD, 2010, Institute of Materia Medica, Chinese Academy of Sciences, P.R. China
  • Jing-Fen Han, PhD, 2011, University of Medicine & Dentistry of New Jersey
  • John Lawrence, PhD, 2011, University of Cincinnati
  • Tuan Dinh, PhD, 2011, University of Cincinnati
  • Jed Kendall, BS, PGY-II, Brigham Young University
  • Meghan Bromwell, PhD, 2011, College of Mount St. Joseph
  • Meghan Brundage, MS, PGY-VI, University of Cincinnati
  • Preeti Tandon, PhD, 2011, University of Cincinnati
  • Mathieu Sertorio, PhD, 2011, University Aix-Marseille II. INSERM, France
  • Kwangmin Choi, PhD, 2010, Indiana University
  • Xiaoli Li, PhD, 2011, Chinese Center for Disease Control and Prevention
  • Surya Amarachintha, PhD, 2011, Bowling Green State University
  • Eri Taniguchi Ishikawa, PhD, PGY-8, Kyoto University, Japan
  • Malav Madhu, MS, 2011, Wright State University
  • Julia Tasset, BS, 2011
  • Ramesh Nayak, PhD, PGY-1, University of Texas at Tyler
  • Michelle Myles, BS, 2011
  • Ashley Ficker, BS, 2009, University of Cincinnati
  • Cuiping Zhang, PhD, PGY-1, Peking Union Medical College, China
  • Ming Liu, PhD, 2010
  • Xun Shang, PhD, 2008
  • Matt Grogg, PhD, 2010, University of Dayton
  • Kristy Stengel, PhD, 2008
  • Ahmed Ramadan Salem Gomaa,
  • Liang Li,
  • Laura Murley,
  • Swarnava Roy, , 2010, NIH
  • Rajat Singhania,
  • Yue Zhang,
  • Harini Raghu, PhD, 2009
  • Diamantis Konstantinidis, PhD
  • Swati Tiwari, , 2011, Delhi, India
  • Archana Shaesta, , 2011, University of Kentucky

Division Collaboration

Division of Bone Marrow Transplant and Immune Deficiency » Stella Davies, Kasiani Myers, and Parinda Mehta
Oxidative stress and bone marrow failure in FA
Division of Endocrinology » Susan Rose
Endocrine defect in FA children
Division of Pediatric Gastroenterology, Hepatology and Nutrition » Kris Steinbrecher
Inflammatory responses in FA hematopoiesis
Division of Ophthalmology » Richard Lang
New DOD grant funded 
Division of Radiology » Diana Lindquist
DOD grant; publication
Division of Oncology » John Perentesis
NIH grant; publication
Division of Developmental Biology » Alex Kuan and Yutaka Yoshida
Co-publications
Division of Molecular Cardiology » Jeff Molkentin
Co-publications
Division of Ophthalmology » Richard Lang
Co-publications
Division of Oncology » Susa Wells
Co-publications
Division of Biomedical Informatics » Jarek Meller
Co-publications
Division of Hematology » Clinton Joiner and Theodosia Kalfa
Sickle Cell Center Grant
Division of Human Genetics » Greg Grabowski
CNS abnormality n murine MPSD type I model as well as Gauche disease model
Division of Reproductive Science » Satoshi Namekawa
Epigenetic regulation in Meiosis and DNA repair
Division of Infectious Diseases » Rhonda Cardin
Role of hematopoietic cells in mechanisms of latency of CMV. My role is to assist in the analysis of M33-mediated signaling by wild type and mutant CMV viruses, and in analysis of akt activation as a M33-mediated survival mechanism, especially in cells of the myeloid lineage, in which CMV establishes latency
Division of Molecular Immunology » Chris Karp
Role of ATF3 in neutrophil functions. My role of to assist in the analysis of the functions of neutrophils lacking ATF3 expression, in particular in neutrophil migration using ex vivo migration assays and in vivo lung inflammatory model. I am also directly helping Dr Karp’s graduate student Nick in experiment design, analysis and interpretation.
Division of Pediatric Ophthalmology » Richard Lang
Provided reagents
Division of Critical Care Medicine » Basilia Zingarelli
Performed experiments
Division of Cellular and Molecular Immunology » Lee Grimes
Xenograft leukemia models
Division of Cellular and Molecular Immunology » Clair Chougnet and Julio Aliberti
Humanizing mice
Division of Bone Marrow Transplantation and Immunodeficiency » Ashish Kumar
Role of Meis1 in AML
Division of Pathology » Gang Huang
Role of RUNX1 in AML
Division of Rheumatology » John Harley
Immune response to EBV in humanized mice
Division of Hematology » Joe Palumbo
Role of thrombin in AML
Division of Cellular and Molecular Immunology » Lee Grimes
Interaction of TIFAB and GFI1
Division of Immunology » David Hildeman
Role of TRAF6 in T cells
Division of Pulmonary Medicine » Jeff Whitsett and Anne Karina Perl
Use flow cytometric analysis to identify lung stem cells:  Investigator on U01 grant (PI:  Whitsett)
Division of Pulmonary Medicine » Bruce Trapnell
Development of stem cell and gene therapies for hereditary Pulmonary Alveolar Proteinosis (Co-PI:  T1 grant funded; Co-PI:  R01 application pending)
Heart Institute » Jeff Robbins and Jeff Towbin
Differentiation of iPSC into cardiac lineages
Division of Molecular Cardiovascular Biology » Stephanie Ware
Studying the role of Zic3 derived cells in the forebrain.  Zic3 is a midline gene and Dr. Ware's lab has generated a Zic3-lacZ mouse that reports dorsal medial midline progenitors in the brain.  My lab is characterizing these cells.    
Division of Neonatology and Pulmonary Biology » Vladimir Kalinichenko
studying the transcription factor Foxm1 in the development of forebrain neurons.  Foxm1 expression is associated with high-grade compared to low-grade gliomas.  My lab is studying the role of this gene in the proliferative “neurogenic” niche in the forebrain 
Division of Oncology » Lionel Chow
Studying the role of Foxm1 in a genetic model of high-grade astrocytoma.
Division of Oncology » Biplab DasGupta
Studying the downstream mechanisms of abnormal Shp2 signaling caused from Shp2-GOF mutations observed in Noonan Syndrome. 
Division of Human Genetics » Mehdi Keddache
Pluripotent cell line characterization for core grant
Division of Human Genetics » Kejian Zhang
Support development of specialized Fanconi Anemia testing
Division of Oncology » Parinda Mehta
Support of Fanconi Anemia Program
Division of Bone Marrow Transplantation and Immune Deficiency » Lisa Filipovich
Support of the SCID-X1 gene transfer trial
Division of Neonatology and Pulmonary Biology » Bruce Trapnell
Grant application to support hPAP gene therapy approach
Division of Ophthalmology » Richard Lang
New DOD grant; publication
Division of Radiology » Diana Lindquist
DOD grant; publication
Division of Oncology » John Perentesis
NIH grant; publication
Division of Bone Marrow Transplantation and Immune Deficiency » Stella Davies, Kasiani Myers, and Parinda Mehta
Oxidative stress and bone marrow failure in FA
Division of Endocrinology » Susan Rose
Endocrine defect in FA children
Division of Pediatric Gastroenterology » Kris Steinbrecher
Hepatology and nutrition:  Inflammatory responses in FA hematopoiesis
Division of Radiology » Dianna Lindquist
Moue MRI imaging
Division of Oncology » Jose Cancelas
Role of Vav3 in acute lymphoblastic leukemia
Division of Neonatal and Pulmonary Biology » Jeffrey Whitsett
NIH grant research on transcriptional control of respiratory epithelial progenitor cells (KLF-5)
Division of Immunobiology » Lee Grimes
NIH grant research on transcriptional control of respiratory epithelial progenitor cells (KLF-5)
Division of Hematology » Theodosia Kalfa, Suvarnama Pushkaran, and Diamantis Konstantinidis
Signaling and cytoskeletal requirements in erythroblast enucleation
Division of Biostatistics & Epidemiology » MiOk Kim
This collaboration involves combining survival analysis with network analysis methodology to study requisite molecular networks driving glioblastoma tumorigenesis under specific oncogenic backgrounds.
Division of Rheumatology » Sherry Thornton
Hemostatic factors and arthritis pathogenesis
Division of Molecular Immunology » Senad Divanovic
Coagulation factors and the pathogenesis of fatty liver disease
Division of Hematology » Joseph Palumbo
This collaboration focuses on mechanism linking procoagulants to malignancy.
Division of Hematology » Eric Mullins
This collaboration focuses on the role of thrombin-mediated proteolysis in neuroinflammatory disease.
Division of Neurosurgery » Charles Stevenson
Xenograft models of pediatric brain tumors
Division of Pathology » Lili Miles
Evaluation of mouse high-grade gliomas
Division of Genetics » Mehdi Keddache and Kejian Zhang
Development of a high-throughput gene chip for the diagnosis of known and discovery of new genetic mutations causing hemolytic anemia due to erythrocyte cytoskeleton disorders, e.g. spherocytosis, elliptocytosis
Division of Rheumatology » Alexei Grom and Michael Barnes
Expression analysis of hemophagocytic lymphohistiocytosis and macrophage activation syndrome
Division of Human Genetics » Kejian Zhang
Mutation analysis of hemaphagocytic lymphohistiocytosis and related disorders
Division of Bone Marrow Transplantation and Immunodeficiency » Lars Wagner
Analysis of minimal residual disease in patients with Ewing Sarcoma
Division of Allergy and Immunobiology; Division of Immunobiology » Kimberly Risma and Michael Jordan
Gene therapy for HLH
Division of Neonatology and Pulmonary Biology » Jeffrey Whitsett and Tim LeCras
Pulmonary pathology in Sickle Cell Disease
Heart Institute » Jeffrey Towbin, Michael Taylor, and Tom Kimball
Cardiac aspects of Sickle Cell Disease

Grants, Contracts, and Industry Agreements

Division Grants

Grant and Contract AwardsAnnual Direct

Andreassen, P

FANCD2 Monoubiquitination in DNA Damage Responses
R01 HL 08558707/08/08-06/30/13$225,000

Azam, M

To Study the Molecular Mechanisms of "BCR/ABL Addiction" in Chronic Myeloid Leukemia
07/01/11-06/30/12$100,000
Mitogenic Activities in Neurofibromatosis
R01 CA15509105/01/12-03/31/17$207,500

Cancelas-Perez, J

Gap Junction Intercellular Communication in Bone Marrow
W81XWH111029604/01/11-09/30/12$33,063
Improving Stem Cell Mobilization by the EGFR Inhibitor Erlotinib
R34 HL 10840302/15/12-01/31/13$98,337
Rac GTPase Inhibition in Chronic Myelogenous Leukemia
R01 HL 08715904/06/09-02/28/13$247,500

Choi, K

Regulation of Cellular Growth and Differentiation
T32 CA 05926812/6/11-12/5/12$49,998

Degen, J

Analysis of Staphylococcus Aureus Host Interactions
R01 AI 02062409/30/10-08/31/12$51,239
Thrombin-Mediated Proteolysis in Neuroinflammatory Disease
R01 HL09612608/01/09-04/30/13$247,500

Degen, J / Malik P

Hemostatic Factors and Sickle Cell Disease
R01 HL 11260301/01/12-11/30/16$250,000

Filippi, M

Regulation of Hematopoietic Stem Cell Self Renewal
R21 HL 10445808/01/10-07/31/12$125,000
Regulation of Neutrophil Migration and Polarity
R01 HL 09067603/01/10-02/28/15$247,500

Flick, M

Mechanisms Linking the Hemostatic Protease Thrombin to Arthritic Disease
R01 AR 05699008/10/09-07/31/14$171,072
Digestive Health Center - Pilot & Feasibility Study
P30 DK 078392 (Bezerra)06/01/12-05/31/13$36,667

Geiger, H

Activated Protein C for Treatment of Radiation Combined Injury
R33 AI 08055709/13/10-08/31/13$79,070

Huang, G

Molecular Mechanisms of Leukemogenesis Mediated by MLL-Partial Tandem Duplication (MLL-PTD)
07/01/11-06/30/13$27,273
Targeting the "Warburg Effect" in Cancer
06/01/12-05/31/13$20,000

Link, K

Environmental Carcinogenesis and Mutagenesis
T32 ES 00725009/01/10-06/30/12$53,494

Malik, P

Ameliorating Sickle Nephropathy and Pulmonary Hypertension
R34 HL 10875208/18/11-06/30/14$150,000
Cincinnati Cell Characterization Core
U01 HL 09999709/01/10-04/30/13$354,674
Development of Safe and Efficient Gene Therapy Strategies
R01 HL 09848901/21/10-12/31/14$48,833
PIGF-HIF 1a-miRNA Axis in Sickle Pulmonary Hypertension
R01 HL11137201/01/12-12/31/16$161,480
Cincinnati Cell Characterization Core - Per assay
U01 HL 09999709/01/10-04/30/13$18,986
Cincinnati Center for Clinical/Translational Sciences & Training
UL1 RR 02631404/03/09-03/31/14$40,294

Meetei, R

Functional and Molecular Characterization of Two New Members of the Bloom Syndrome Complex
07/01/10-06/30/12$27,272

Mulloy, J

Next Generation DNMT-1 Depletion Therapy for Leukemia
W81XWH-09-1-067109/01/09-09/01/12$141,405
Novel Therapeutic Target in Leukemia Stem Cells
07/01/10-06/30/12$100,000
Rac Signaling in MLL Leukemia
07/01/10-06/30/15$104,762

Nassar, N

Ras, Cycling and Inhibition
R01 CA11561103/01/11-02/28/13$108,236

Olshavsky, N

Regulation of Cellular Growth and Differentiation
T32 CA5926812/06/10-12/05/12$32,303

Pan, D

Genetic Therapy for CNS Manifestations in MPS I via BBB-Targeted Protein Delivery
R01 NS 06433009/30/08-08/31/13$214,375

Pang, Q

Role of FA Proteins in Hematopoiesis
R01 HL 07671204/01/10-03/31/15$250,000
Role of Tumor Necrosis Factor in Leukemogenesis
07/01/08-06/30/13$103,115
Targeted Improvement in Stem Cell Therapy for Leukemia and Bone Marrow Failure Syndromes
R01 CA 15753702/01/11-12/31/15$207,500

Patel, A

Identification and study of Novel Genes Critical to survival of MPNSTS
W81XWH111014406/01/11-05/31/13$50,000

Ratner, N

Cincinnati Center for Neurofibromatosis Research
P50 NS 05753109/15/08-06/30/13$1,033,483
Ratner, NProject A$48,069
Cripe, TProject B$106,147
Rizvi, TProject C$81,328
Perentesis, JProject 1$297,055
Ratner, NProject 2$224,070
Ratner, NProject 3$276,814
$
Mitogenic Activities in Neurofibromatosis
R01 NS 02884009/15/11-07/31/16$231,250
Modelling Brain Defects in NF1
W81XWH101011604/01/10-03/31/13$251,091

Starczynowski, D

Deregulation of TIFAB in Myelodysplastic Syndrome
07/01/11-06/30/14$50,000
Regulation and Function of TIFAB in Myelodysplastic Syndrome
W81XWH111046806/01/11-05/31/14$132,295
Identification and Characterization of Genes in del(5q) Myelodysplastic Syndrome
R01 HL11110312/05/11-11/30/16$250,000

van der Loo, J

AKTA Ready Liquid Chromatography System
S10 RR 03172107/01/11-06/30/12$175,119

Varney, M

Environmental Carcinogenesis and Mutagenesis
T32 ES 00725005/01/12-04/30/14$49,198

Wu, J

STAT3 in Neurofibroma Tumorigenesis and Therapy
W81XWH111025907/01/11-06/30/14$129,364
STAT3 in Neurofibroma Tumorigenesis and Therapy
08/01/10-07/31/12$49,205

Zheng, Y/ Geiger, H

Lineage Determination and Tissue HomeOstasis in the Aged Hematopoietic System
R01 AG 04011808/01/11-07/31/16$225,000

Zheng, Y

Cincinnati Center for Excellence in Molecular Hematology
P30 DK 09097109/30/10-06/30/15$482,569
Zheng, YAdmin Core$89,909
Grabowsky, GGenomics and Genetics Core$63,000
Cancelas, JCell Analysis and Sorting Core$65,112
Malik, PTranslational Core$165,412
Mulloy, JXenotransplant and Transgenic Core$68,766
Zheng, YSummer Students$30,370
Rac GTPase-Specific Small Molecular Inhibitors
R01 CA 14134103/24/09-01/31/14$165,237
Training Program in Pediatric Hematologic and Oncologic Diseases
T32 HL 09180509/01/08-08/31/13$164,652
Rac GTPases in the Mammalian Brain Development
R01 NS 05643507/01/08-06/30/12$165,237

Zheng, Y / Mulloy J

Targeting Cdc42 in Leukemia Stem Cells
R01 CA 15054703/10/10-01/31/15$201,275
Current Year Direct$8,138,423
Industry Contracts

Flick, M

$53,159

Malik, P

$4,719

Mulloy, J

$63,229
Current Year Direct Receipts$121,107
Service Collaborations

Grassman, E

$183,361
$11,593
Current Year Direct$194,954
Funded Collaborative Efforts

Malik, P

Macrophage-based Human Gene Therapy for Hereditary PAP
Trapnell, B12/15/10-11/30/125%
Role of Anti-GM-CSF Antibodies in Myeloid Cell Function
Trapnell, B04/01/11-03/31/165%

Andreassen, P

DNA Damage Response Pathways in Meiotic Sex Chromosome Inactivation
Namekawa, F08/01/11-07/31/167.5%
Total$8,454,484