Target Amplification Protocols
The Core offers multiple target amplification protocols for the preparation of biotin- labeled target. The following table indicates which protocols are available based upon one’s preference of array type. Furthermore, when selecting a protocol one must consider both the quality and quantity of the starting RNA.
Array Types
|
Amplification protocols and
Labeling fee per sample
|
Fee
|
Minimum RNA Quality |
Minimum
amount of
RNA
|
Affymetrix
Gene and
Exon Arrays |
Affymetrix Whole Transcript
Sense Target Labeling
Assay* |
$249 |
Intact |
200ng |
| |
WT-Ovation Pico
+Exon Module
|
$354 |
Intact or Degraded
|
1ng |
| |
WT-Ovation FFPE System
+Exon Module
|
$387 |
Severely degraded |
50 ng of
total FFPE RNA |
Illumina
BeadChip
|
Illumina TotalPrep
|
$187 |
Intact |
300ng |
| |
Epicentre TargetAmp 1-
Round System
|
$255 |
Intact |
300ng |
| 3' Affymetrix
Expression
Arrays
|
Standard Affymetrix
|
$295 |
Intact |
1ug
|
| |
Ovation RNA Amplificiation V2
|
$311 |
Intact |
5ng |
| |
WT-Ovation Pico
|
$336 |
Intact or
Degraded
|
1ng |
| |
WT-Ovation FFPE System
|
$369 |
Severely Degraded
|
50 ng of
total FFPERNA
|
| |
Epicentre TargetAmp 1-
Round System
|
$255 |
Intact
|
300ng
|
* When using the Affymetrix Whole Transcript Sense Target Labeling Assay to prepare target cDNA for hybridization to an Exon ST 1.0 Array, Affymetrix strongly recommends the use of the rRNA Reduction Protocol. The minimum required amount of starting total RNA is 1ug. There will be an additional $25 per sample fee.
