Research Flow Cytometry Core

Resources

Links 

• Purdue University Cytometry Laboratories - A great resource for all things flow.
• BDBiosciences Multicolor Flow Cytometry Tools - Several tools for selecting the best fluorochromes.
• Invitrogen's Fluorescence SpectraViewer - For comparing the spectra of fluorophores from Molecular Probes.
• Invitrogen's Fluorescence Tutorials - Five tutorials on the basics of fluorescence, understanding fluorescence spectra, optics, flow cytometry and data analysis.
• Amnis ImageStream.

 
Protocols in flow cytometry

• Current Protocols in Cytometry (online subscription through the Pratt Library at Cincinnati Children's)
• Cell staining for mature hematopoietic cell populations 

 
Basic tutorials to design properly a flow cytometry experiment:

• Guideline for Designing an Experiment in Multicolor Flow Cytometry
• Guideline for Proper Compensation in Flow Cytometry
  

 
Fluorochromes

• Fluorochromes Description
• Fluorochromes Brightness Chart 

 

Glossary: 

• Glossary of terms 
• Frequently asked questions 
  

Research Cores

Research Flow Cytometry Core

• Overview
• About Us
• Our Staff
• Analyzers
• Cell Sorting
• ImageStream
• Software
• Core Equipment
• Resources
• Glossary
• Frequently Asked Questions