Cell Sorting
The RFCC at Cincinnati Children’s operates three cell sorters for the simultaneous purification of up to four populations to be used for downstream applications. If you are a new client, please make an appointment with Monica DeLay to discuss your experimental needs. You must bring a signed billing sheet with your samples to each sort.
| | | | Number of Fluorescence PMTs |
| Instrument Name* | Instrument Make/Model | Location** | Blue (488nm) | Red (635nm) | Violet (405nm) | Yellow-Green (561nm) | UV (335nm) |
| Ariel | BD FACSAria II | R5001 | 5 | 3 | 2 | | 2 |
| Arnie | BD FACSAria II | R5001 | 2 | 3 | 2 | 5 | 2 |
| MoFlo | Beckman Coulter MoFlo XDP | R5529 | 5 | 3 | 2 | | |
Cell Sorting Information |
Location
R5001: FACSAria's
R5529: MoFlo XDP Hours
9 am to 6 pm Monday-Friday To Schedule
513-636-2770 - FACSAria's
513-636-2054 - MoFlo XDP |
|
*Click on the instrument name for the configuration.
**BL2 rooms. Doors must be kept closed at all times and appropriate PPE worn while in the instrument rooms.
Sample tube options include: 1.1 ml tubes (Neptune cat# 2601.S); 12x75 mm Falcon tubes; 15 ml conicals.
Collection options include: 1.5 ml tubes; 12x75 mm Falcon tubes; 15 ml conicals; multiwell plates; slides.
Scheduling
Call 513-636-2770 (FACSAria's) or 513-636-2054 (MoFlo) to schedule a time between 9 am and 6 pm Monday-Friday. We will fill out a scheduling form for each appointment. Be prepared to provide details related to your sort. All appointments, modifications and cancelations are confirmed via email. Click here to view the calendar.
Sample preparation
Check out the Guidelines for Sorting for information on sample preparation, collection, etc.
Sorting fees
The sorting fees are $85 / hour + one-hour setup. A billing form must be filled out and brought with you each time you sort.
Cancellation / late policy
You must call to cancel your appointment. All cancellations will be confirmed via email to the client. You will be charged for the sort if you cancel less than 24 hours before the scheduled time. For Monday sorts, you must cancel by noon the Friday before. If you are late, you will be charged for the time you scheduled. If your sort time runs over, you will be charged for the time you used.
Infectious cell sorting
The five-laser FACSAria II (Arnie) and the MoFlo XDP are contained in class II biosafety cabinets for sorting of potentially infectious samples. Contact Monica DeLay for more information on infectious cell sorting.
After your sort
Tell us how we’re doing! After your appointment, fill out a survey to provide feedback on your sort.
