• Research Flow Cytometry Core

    The Research Flow Cytometry Core (RFCC) is a shared resource laboratory serving the Cincinnati Children's research communities and surrounding institutions by providing a central location for flow cytometry instrumentation, training and education. We offer a number of services for both internal and external scientists.

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    What's New

    Single-Cell Systems Biology Workshop
    Thursday, February 5th - 8:15AM –12:30PM - S1.203

    Hosted by the RFCC and Fluidigm, with the following speakers agenda.

    Applications of Single Cell RNA Sequencing for Lung Development and Disease
    Dr. Jeff Whitsett, CCHMC, Co-Director, Perinatal Institute, Chief, Section of Neonatology, Perinatal and Pulmonary Biology, Interim Director, CCRF, Basic Science

    Every Cell Has a Story – Igniting a Revolution Through Single-cell Systems Biology
    Dr. Maxine McClain, Field Application Scientist, Fluidigm Corporation 

    Single Cell Gene Expression Analysis of Cancer Stem Cells and Circulating Tumor Cells: Applications and Challenges
    Dr. Ebrahim Azizi, Visiting Scholar (Professor of TUMS) in Cancer Stem Cells Research at the University of Michigan

    Identification of Cell Types and Networks in Heterogeneous Populations of Interacting Cell Types
    Dr. Bruce Aronow, CCHMC, Co-director, Computational Medicine Center, Professor, UC Department of Pediatrics

    Understanding Biological Heterogeneity through Mass Cytometry
    Dr. Maxine McClain, Field Application Scientist, Fluidigm Corporation 

    Investigating the Role of Autophagy in Aging Using Proteomics and Mass Cytometry Strategies
    Michelle Kuhns from Dr. Edgar Arriaga’s Lab from the University of Minnesota

    Refreshments will be provided. Please RSVP to CDJamoos@fluidigm.com

    If you cannot attend, click HERE to watch a live stream or an archive of the workshop.  

    Introduction to Flow Cytometry

    New users need to complete this class prior to hands-on instrument training. This monthly class is 2 hours and covers instrument components, analysis, compensation and experimental basics. Register for this class on the ELM (Cincinnati Children's users) or email Monica DeLay for external clients. See below for future dates, times and locations. 

    Date     TimeLocation
    1/27/20152:00-4:00PMS8.125
    2/24/20152:00-4:00PMS5.125
    3/24/20152:00-4:00PMS10.130
    4/28/20152:00-4:00PMS5.125
    5/26/20152:00-4:00PM S5.125
    6/23/20152:00-4:00PMS10.130
  • Children’s employees: From the lobby of the S building, take an elevator to the 5th floor. Turn left out of the elevator bay and proceed to the R building. Our desks are located in R5543 and the office is in R5564. The instrument rooms are R5543, R5524, R5529, R5001 and R5087A. From the R building, take an elevator to the 5th floor. Turn left and then right and proceed down the hallway to our area (see room numbers above). 

    Non-Children’s employees: If you are driving to Cincinnati Children’s, please view directions, maps and information about parking. See building and room numbers above.

    When publishing data that is generated using our facility, it is necessary to properly acknowledge us in the publication since we are partially supported by three NIH P30 grants through the Division of Rheumatology’s Center for Rheumatic Disease Research (NIH AR47363), Division of Gastroenterology’s Digestive Health Center (NIH DK78392), and the Division of Experimental Hematology and Cancer Biology’s Center for Excellence in Molecular Hematology (NIH DK90971).

    See below for example statements to be used for this purpose and feel free to cite a specific staff member if appropriate.

    To acknowledge our assistance:
    We would like to acknowledge the assistance of the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center, supported in part by NIH AR-47363, NIH DK78392 and NIH DK90971.

    To acknowledge our facility:
    All flow cytometric data were acquired using equipment maintained by the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center, supported in part by NIH AR-47363, NIH DK78392 and NIH DK90971.

  • General Information

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    Location

    Office: R5543 (Lab desks), R5564 (Main Office)

    Analyzer rooms:
    R5524: FACSCalibur's, LSR's
    R5087A: FACSCanto's
    R5543: ImageStreamX 

    Sorting Rooms:
    R5001: FACSAria's
    R5529: MoFlo XDP

    Luminex:
    R5543

    Analyzer Hours
    24 / 7 for those who have been trained by our staff

    Sorting Hours
    9 am to 6 pm Monday-Friday

    Contact Us
    General information, help and analyzer training:
    513-636-3575

    Cell sorting appointments:
    513-636-2770 - FACSAria's
    513-636-2054 - MoFlo XDP

    Analyzer rooms:
    513-636-3332