• Research Flow Cytometry Core

    The Research Flow Cytometry Core (RFCC) is a shared resource laboratory serving the Cincinnati Children's research communities and surrounding institutions by providing a central location for flow cytometry instrumentation, training and education. We offer a number of services for both internal and external scientists.

    What's New

    ORVCA Users Meeting

    The next ORVCA monthly user’s meeting is on Wednesday, April 27th from 2-3 pm in building S, Room S5.125. Ray Eby, PhD, Particle Metrix, Inc., will be presenting "Exploring Sub-populations in Samples of Extracellular Vesicles Using Multivariate Analysis of NTA Data." Refreshments will be served.

    Advanced Multicolor Panel Design Class

    The next multicolor panel design class will be on Thursday, June 30th from 2-4 pm in S3.125. This class is a complement to the Introduction to Flow Cytometry class and is held quarterly for users who need help building good flow cytometry panels. Register for this class on the ELM or email Javid Mohammed for external clients.

    Introduction to Flow Cytometry

    New users need to complete this class prior to hands-on instrument training. This monthly class is 2 hours and covers instrument components, analysis, compensation and experimental basics. Register for this class on the ELM (Cincinnati Children's users) or email Monica DeLay for external clients. See below for future dates, times and locations. 

    Date     TimeLocation
    April 26, 20162:00-4:00PMS5.125
    May 31, 20162:00-4:00PMS5.125
    June 28, 20162:00-4:00PMS5.125
    July 26, 20162:00-4:00PMS5.125
    Aug. 30, 20162:00-4:00PMS5.125
    Sept. 27, 20162:00-4:00PMS5.125
    Oct. 25, 20162:00-4:00PMS5.125
    Nov. 29, 20162:00-4:00PMS5.125
    Dec. 27, 20162:00-4:00PMS5.125
  • Children’s employees: From the lobby of the S building, take an elevator to the 5th floor. Turn left out of the elevator bay and proceed to the R building. Our desks are located in R5543 and the office is in R5564. The instrument rooms are R5543, R5524, R5529, R5001 and R5087A. From the R building, take an elevator to the 5th floor. Turn left and then right and proceed down the hallway to our area (see room numbers above). 

    Non-Children’s employees: If you are driving to Cincinnati Children’s, please view directions, maps and information about parking. See building and room numbers above.

    When publishing data that is generated using our facility, it is necessary to properly acknowledge us in the publication since we are partially supported by three NIH P30 grants through the Division of Rheumatology’s Center for Rheumatic Disease Research (NIH AR47363), Division of Gastroenterology’s Digestive Health Center (NIH DK78392), and the Division of Experimental Hematology and Cancer Biology’s Center for Excellence in Molecular Hematology (NIH DK90971).

    See below for example statements to be used for this purpose and feel free to cite a specific staff member if appropriate.

    To acknowledge our assistance:
    We would like to acknowledge the assistance of the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center, supported in part by NIH AR-47363, NIH DK78392 and NIH DK90971.

    To acknowledge our facility:
    All flow cytometric data were acquired using equipment maintained by the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center, supported in part by NIH AR-47363, NIH DK78392 and NIH DK90971.

  • General Information

    Scheduling:

    Location

    Office: R5543 (Lab desks), R5564 (Main Office)

    Analyzer rooms:
    R5524: FACSCalibur's, LSR's
    R5087A: FACSCanto's
    R5543: ImageStreamX 

    Sorting Rooms:
    R5001: FACSAria's
    R5529: MoFlo XDP

    Luminex:
    R5543

    Analyzer Hours
    24 / 7 for those who have been trained by our staff

    Sorting Hours
    9 am to 6 pm Monday-Friday

    Contact Us
    General information, help and analyzer training:
    513-636-3575

    Cell sorting appointments:
    513-636-2770 - FACSAria's
    513-636-2054 - MoFlo XDP

    Analyzer rooms:
    513-636-3332