• Core Equipment

    AutoMACS Pro

    The AutoMACS Pro separator by Miltenyi Biotec is a benchtop automated magnetic cell sorter for the isolation of many cell types from multiple species. This instrument is particularly useful for enriching cell populations prior to cell sorting.  For rare populations, using the AutoMACS in conjunction with cell sorting results in better purity and requires less processing time.

    If you would like training, contact Alyssa Sproles (636-3575).  Buffers are provided and columns are changed on a regular basis. Contact us for more information or questions regarding the applications of the AutoMACS Pro separator. People using the AutoMACS must provide their own cell separation reagents. 

    Scheduling
    After training, you can schedule time using the calendar next to the instrument.  Note: priority may be given to users sorting time-sensitive patient samples.

    Fees
    Pricing for internal clients can be found on Centerlink.  For external clients, please contact us.

    Technical Support
    Need help?  We can be reached in R5543, R5564 or at 513-636-3575.

    Cellometer Vision

    An automated cell counter by Nexcelom Bioscience that measures brightfield and fluorescence in up to two channels (510-570 and 570-650nm).

    Applications include:

      • Cell counts and size distribution
      • Determination of cell viability with dyes (e.g., PI, trypan blue, acridine orange)
      • Quantifying fluorescent protein expression (e.g., GFP, YFP)
      • Counting WBCs in whole blood
      • Annexin V for detection of apoptosis

    Minimum sample concentration: 1-2e5 / ml

    Minimum sample volume: 20ul

    Fees

    Pricing for internal clients can be found on Centerlink.  For external clients, please contact us.

  • AutoMACS Pro Information

    equipment-visual1-300

    Location
    R5543

    Hours
    24/7 for those who have been trained by our staff

    Contact
    Alyssa Sproles
    513-636-3575

  • Cellometer Vision Information

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    Location
    R5001

    Contact
    Monica DeLay      
    513-636-3575