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The ImageStreamX imaging flow cytometer combines the visual power of microscopy with the speed and sensitivity of flow cytometry by digitally imaging cells directly in flow.
If you would like to be trained on the ImageStream, contact Nicole White at 513-636-3575 or Amanda.White@cchmc.org.
After training, you can schedule time using the Research Equipment Booking System. If you are a non-Cincinnati Children’s employee, email firstname.lastname@example.org to gain access to the booking system.
Download the Sample Preparation Guide for information on experimental design and the protocol for cell preparation. Samples are run using 1.5 ml microfuge tubes. Visit the Amnis website and register for an online account for access to more tips for applications and protocols.
The instrument is located within R5543, which is locked after working hours. If you need access to this room outside of the normal working day, please let us know. This is a BL2 room.
ImageStream use is $30 / hour. If you would like RFCC staff to run samples for you, contact Nicole White at 513-636-3575 or Amanda.White@cchmc.org to discuss your specific needs.
Need help? We can be reached in R5543 or at 513-636-3575.
Alternatively, call Amnis at 1-800-730-7147, option 2. Support is available 11 am-9 pm M-F.
A project drive has been set up for ImageStream data called “RFCC-ImageStream” (Cincinnati Children’s users only). Access will be given after training. During acquisition the data will be stored on the analysis computer. You will then transfer your data to your folder on RFCC-ImageStream where it can be analyzed from any computer running the IDEAS software application.
Request access to one of our project drives at https://bmirdsdp.cchmc.org/DAP/
For analysis of ImageStream data, there is a PC workstation next to the instrument with the IDEAS software package. If you would like to install a copy of IDEAS on your PC, you can do so at www.amnis.com after registering for an account. IDEAS will also run on a Mac with ’parallels’ capability.
Subscribe to Imagestream-users email list by completing this online form to receive updates and announcements.
Hours24/7 for those who have been trained by our staff
Contact UsGeneral information, help and training:513-636-3575
Contact Nicole WhiteTo Schedule TimeResearch Equipment Booking System
3333 Burnet Avenue, Cincinnati, Ohio 45229-3026 | 1-513-636-4200 | 1-800-344-2462 | TTY:1-513-636-4900
New to Cincinnati Children’s or live outside of the tri-state area? 1-877-881-8479
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