Research Flow Cytometry Core

  • ImageStreamX

    The ImageStreamX imaging flow cytometer combines the visual power of microscopy with the speed and sensitivity of flow cytometry by digitally imaging cells directly in flow. 

    Features of our instrument:

    • Lasers - 405 nm, 488 nm, and 658 nm.  Plus a 785 nm laser for Scatter.
      12 Image Channels - Two cameras, each with a brightfield channel leaves up to 10 channels available for fluorescence detection. See Configuration.
    • MultiMag - This option provides 20× and 60× objective lenses in addition to the standard 40× lens for greater imaging flexibility and improved resolution.
    • Extended Depth of Field - EDF™ extended depth of field technology uses a combination of specialized optics and unique image processing algorithms to project all structures within the cell into one crisp plane of focus.
    • Full Color Brightfield - The Full Color Brightfield option provides a full spectrum brightfield light source that allows the ImageStreamX system to replicate the RGB brightfield imagery of a microscope.
      For more information visit the Amnis website.

    Training

    If you would like to be trained on the ImageStreamX, contact Jonathan Schick at 513-636-3575 or Jonathan.Schick@cchmc.org.

    Scheduling

    After training, you can schedule time using the Research Equipment Booking System. If you are a non-Cincinnati Children’s employee, email help@bmi.cchmc.org to gain access to the booking system.

    Sample Preparation and Protocols

    Download the Sample Preparation Guide for information on experimental design and the protocol for cell preparation. Samples are run using 1.5 ml microfuge tubes. Visit the Amnis website and register for an online account for access to more tips for applications and protocols.

    Location

    The instrument is located within R5543, which is locked after working hours. If you need access to this room outside of the normal working day, please let us know. This is a BL2 room.

    Fees

    ImageStreamX use is $30 / hour. If you would like RFCC staff to run samples for you, contact Jonathan Schick at 513-636-3575 or Jonathan.Schick@cchmc.org to discuss your specific needs.

    Technical Support

    Need help? We can be reached in R5543 or at 513-636-3575.

    Alternatively, call Amnis at 1-800-730-7147, option 2. Support is available 11 am-9 pm M-F.

    Data Storage

    A project drive has been set up for ImageStreamX data called “RFCC-ImageStream” (Cincinnati Children’s users only). Access will be given after training. During acquisition the data will be stored on the analysis computer. You will then transfer your data to your folder on RFCC-ImageStream where it can be analyzed from any computer running the IDEAS software application.

    Request access to one of our project drives at https://bmirdsdp.cchmc.org/DAP/

    Software

    For analysis of ImageStreamX data, there is a PC workstation next to the instrument with the IDEAS software package. If you would like to install a copy of IDEAS on your PC, you can do so at www.amnis.com after registering for an account. IDEAS will also run on a Mac with ’parallels’ capability.

    Subscribe to our email list

    Subscribe to Imagestream-users email list by completing this online form to receive updates and announcements.