Research Flow Cytometry Core

Related Resources

Flow Cytometry Links and Tools

• Purdue University Cytometry Laboratories − A great resource for all things flow.
• BDBiosciences Multicolor Flow Cytometry Tools − Several tools for selecting the best fluorochromes. 
• Invitrogen’s Fluorescence Tutorials − Five tutorials on the basics of fluorescence, understanding fluorescence spectra, optics, flow cytometry and data analysis.
• BioLegend Webtools − Tools for developing panels for multicolor assays.
• Beckman Coulter − Their website section dedicated to flow cytometry.

Spectra Viewers

• BD Biosciences
• Life Technologies
• eBioscience
• BioLegend

Protocols in flow cytometry

• Current Protocols in Cytometry (online subscription through the Pratt Library at Cincinnati Children’s)

• OMIPs (Optimized Multicolor Immunofluorescence Panels) – A publication type in Cytometry Part A to communicate complex reagent panels for interrogating phenotypic and functional characteristics of cell populations.

  Publishing Flow Cytometry Data − MIFlowCyt (Minimum Information about a Flow Cytometry experiment)  A standard for outlining the minimum information required to report the experimental details of flow cytometry experiments.

Basic tutorials for designing a flow cytometry experiment

• Guideline for Designing an Experiment in Multicolor Flow Cytometry
• Guideline for Proper Compensation in Flow Cytometry

Fluorochromes

• Fluorochrome Descriptions
• Fluorochrome Brightness Chart

Other Flow Cytometry Resources in Cincinnati

• Visit the Shriners Flow Core website for information about satellite sites located at Shriners and University of Cincinnati.