• Target Amplification Protocols

    The core offers multiple target amplification protocols for the preparation of biotin-labeled target. The following table indicates which protocols are available based upon one’s preference of array type.  Furthermore, when selecting a protocol one must consider both the quality and quantity of the starting RNA.

    Array Types

    Amplification Protocols and
    Labeling Fee Per Sample

    Fee

    Minimum RNA Quality

    Amount of RNA

    Affymetrix
    Gene and
    Exon Arrays

    Ambion® WT Expression

    $275    

    Intact

    100 - 300 ng

    Ovation Pico WTA
    +WT Ovation Exon Module     

    $354

    Intact or degraded

    1 - 20 ng

    WT-Ovation FFPE System
    + WT Ovation Exon Module

    $387

    Severely degraded

    50 ng of total FFPE RNA

    Illumina
    BeadChip

    Epicentre TargetAmp 1-Round System

    $255

    Intact

    300 - 500 ng

    3’  Affymetrix
    Expression
    Arrays

    Genechip 3’ IVT Express Kit

    $295

    Intact

    100 - 300 ng

    Ovation RNA Amplification V2

    $311

    Intact

    5 - 20 ng

    Ovation Pico WTA

    $336

    Intact or degraded

    1 - 20 ng

    WT-Ovation FFPE System

    $369

    Severely degraded

    50 ng of total FFPE RNA

    Epicentre TargetAmp 1-Round System

    $255

    Intact

    300 - 500 ng