• Target Amplification Protocols

    The core offers multiple target amplification protocols for the preparation of biotin-labeled target. The following table indicates which protocols are available based upon one’s preference of array type. Furthermore, when selecting a protocol one must consider both the quality and quantity of the starting RNA.

    Please contact us for fee information.

    Array Types

    Amplification Protocols and
    Labeling Fee Per Sample

    Minimum RNA Quality

    Amount of RNA

    Affymetrix
    Gene and
    Exon Arrays

    Ambion® WT Expression

    Intact

    100 - 300 ng

    Ovation Pico WTA
    +WT Ovation Exon Module     

    Intact or degraded

    1 - 20 ng

    WT-Ovation FFPE System
    + WT Ovation Exon Module

    Severely degraded

    50 ng of total FFPE RNA

    Illumina
    BeadChip

    Epicentre TargetAmp 1-Round System

    Intact

    300 - 500 ng

    3’  Affymetrix
    Expression
    Arrays

    Genechip 3’ IVT Express Kit

    Intact

    100 - 300 ng

    Ovation RNA Amplification V2

    Intact

    5 - 20 ng

    Ovation Pico WTA

    Intact or degraded

    1 - 20 ng

    WT-Ovation FFPE System

    Severely degraded

    50 ng of total FFPE RNA

    Epicentre TargetAmp 1-Round System

    Intact

    300 - 500 ng