Target Amplification Protocols
The core offers multiple target amplification protocols for the preparation of biotin-labeled target. The following table indicates which protocols are available based upon one’s preference of array type. Furthermore, when selecting a protocol one must consider both the quality and quantity of the starting RNA.
Array Types | Amplification Protocols and
Labeling Fee Per Sample | Fee | Minimum RNA Quality | Amount of RNA |
Affymetrix
Gene and
Exon Arrays | Ambion® WT Expression | $275 | Intact | 100 - 300 ng |
| Ovation Pico WTA
+WT Ovation Exon Module | $354 | Intact or degraded | 1 - 20 ng |
| WT-Ovation FFPE System
+ WT Ovation Exon Module | $387 | Severely degraded | 50 ng of total FFPE RNA |
Illumina
BeadChip | Epicentre TargetAmp 1-Round System | $255 | Intact | 300 - 500 ng |
3’ Affymetrix
Expression
Arrays | Genechip 3’ IVT Express Kit | $295 | Intact | 100 - 300 ng |
| Ovation RNA Amplificiation V2 | $311 | Intact | 5 - 20 ng |
| Ovation Pico WTA | $336 | Intact or degraded | 1 - 20 ng |
| WT-Ovation FFPE System | $369 | Severely degraded | 50 ng of total FFPE RNA |
| Epicentre TargetAmp 1-Round System | $255 | Intact | 300 - 500 ng |
