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The Gene Expression Core has the following guidelines for researchers preparing RNA for microarray experiments:
Ideal starting total RNA amounts (not mRNA) depend on the target amplification protocol requested.
Before starting an experiment, the core facility performs a quality control test using the Agilent Bioanalyzer. The available assays use minimal amounts of RNA per sample. The assays separate the total RNA by size. Samples of high integrity should produce sharp, distinct 28S and 18S rRNA peaks with no evidence of degradation. Investigators should submit two microliters of sample in a well labeled 0.5 ml microcentrifuge tube. Include a printout listing the name of the samples, concentrations, and one’s contact information. Investigators may use this service without submitting samples for a microarray experiment.
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