• Library Production

    The Lenti-shRNA Library Core uses two fully automated Biomek FXP array robots to produce libraries. The robots feature online incubator and plate carousels, automated plate labeling and sealing and HT DNA isolation and quantification.

    Our team takes a number of steps to maintain high-quality control during the production process. We offer online-plate mapping, orientation and identity. We conduct DNA quantification and normalization using the PicoGreen assay and Nanodrop. We offer restriction digest and HT DNA sequencing, and we estimate virus titering using puromycin viability assay.

  • Flow chart of library production.

    click to enlarge