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The Lenti-shRNA Library Core uses two fully automated Biomek FXP array robots to produce libraries. The robots feature online incubator and plate carousels, automated plate labeling and sealing and HT DNA isolation and quantification.
Our team takes a number of steps to maintain high-quality control during the production process. We offer online-plate mapping, orientation and identity. We conduct DNA quantification and normalization using the PicoGreen assay and Nanodrop. We offer restriction digest and HT DNA sequencing, and we estimate virus titering using puromycin viability assay.
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If you are interested in using our services or have any questions, contact us via email: LentiCore@cchmc.org.
3333 Burnet Avenue, Cincinnati, Ohio 45229-3026 | 1-513-636-4200 | 1-800-344-2462 | TTY: 1-513-636-4900
New to Cincinnati Children’s or live outside of the Tristate area? 1-877-881-8479
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