Overview
The Mass Spectrometry Laboratory provides an analytical resource that focuses on the application of mass spectrometry and allied chromatographic approaches to the analysis of complex biological samples.
The facility is equipped with a high resolution VG Autospec Q GC-MS-MS instrument, a Micromass LC Quattro Electrospray Triple quadrupole instrument, and a Fisons MD-800 benchtop instrument. These instruments are complemented with four capillary column gas chromatography instruments and four high pressure liquid chromatography (HPLC) systems having ultraviolet, fluorescence and electrochemical detectors that are available for method development and analytical support.
The laboratory has an active independent research program applying mass spectrometry to the diagnosis and treatment of disease states, and to the understanding of developmental biochemistry, physiology and pathophysiology. The mass spectrometer provides structural information based upon the molecular weight and fragmentation of the molecule, offers high sensitivity and precision for quantification. The techniques can distinguish stable-isotopically labeled analogs by their differences in mass and are therefore a powerful tool for tracer and kinetic studies in pediatrics, where the use of radioactive tracers can be avoided.
The facility is recognized as a leader in the area of cholesterol, steroid and bile acid metabolism and consequently has built a strong national and international program in this area of research. Training in analytical biochemistry is offered to clinical fellows and graduates, and a strong emphasis on method development has led to collaborations with investigators in other divisions within the Research Foundation. The Mass Spectrometry Laboratory also supports part of the core for the Natioanl Insititutes of Health Program Project Grant on cholesterol absorption awarded to the Department of Pathology at the University of Cincinnati College of Medicine.
How to Reach Us
For more information, contact Kenneth D. Setchell, Ph.D., 513-636-4548.
