Overview
The Proteomics Laboratory within the University of Cincinnati/Genome Research Institute is fully equipped with protein separation and mass spectrometry equipment and the trained personnel to perform comparative analyses of complex protein mixtures and the identification of differentially-expressed proteins. Proteomics laboratory personnel also offer guidance to the research community regarding the feasibility of a given approach and/or advice on proteomics experimental design to enhance the probability of success.
Research expertise in the Proteomics Laboratory is divided into two subcategories:
- The electrophoresis section assists investigators in evaluating protein expression changes that occur in a biological system, such as during disease development and progression, stress or drug exposure or during normal cell and tissue development. The identification of changing protein expression patterns can provide important information about pathways involved in these processes and can lead to the identification of new protein markers for the diagnosis or the treatment of diseases.
- The mass spectrometry section provides the identification of differentially-expressed proteins and the characterization of protein modifications (e.g. phosphorylation, glycosylation). Furthermore the laboratory is currently developing quantitative proteomics methods in which mass spectrometry can be used to both identify the proteins and quantify the changes.
For more information, contact Ken Greis, (ken.greis@uc.edu), or visit the University of Cincinnati Proteomics Web site.
