Protein Characterization and Proteomics

Services and Equipment

The Proteomics Laboratory at the University of Cincinnati/Genome Research Institute provides services to investigators in the analysis and identification of complex protein mixtures using Two-Dimensional Gel Electrophoresis and Mass Spectrometry. For protein identification and characterization, the laboratory is equipped with four mass spectrometers, offering a set of complementary technologies to address the different needs of the investigator.

The laboratory offers the following services:

  • 2-D gel electrophoresis, protein staining, and analysis of differentially-expressed proteins.
  • Assistance to investigators in the preparation of cell or tissue samples.
  • A complete suite of standard protein electrophoresis services including:
  • small-format and large-format denaturing electrophoresis.
  • native one-dimensional gel electrophoresis.
  • Complete set of Protein Mass Spectrometry services:
  • Identification of post-translational protein modifications.
  • Identification of interaction protein complexes from pull-down experiments
  • Mass confirmation of purified proteins.
  • Sequence confirmation of synthetic peptides.

Services can be provided as a simple fee-for-service or via project collaborations with direct support for core investigators within grants. Please contact Ken Greis to discuss your project and core fees. Current fee structures for standard services are available on the UC Office of Research and Graduate Education Proteomics Website.

How to Reach Us

For more information, contact Ken Greis, (ken.greis@uc.edu), or visit the University of Cincinnati Proteomics Web site.