Core Resources
The Division of Developmental Biology at Children's Hospital Research Foundation of Cincinnati continues to administer the following core resources.
Transgenic Core Facility
The transgenic mouse core facility operates under the direction of Richard Lang, PhD, to generate transgenic mice by pronuclear microinjection and chimeric mice using embryonic stem cells. The facility has also undertaken several new initiatives including cryopreservation of transgenic lines and start-to-finish gene targeting. The facility provides consultation services and instruction in basic molecular biology techniques to researchers and trainees beginning to use these tools. Questions should be directed to Sandy Falcone.
Affymetrix GENECHIP" Microarray Core Facility
The Affymetrix GENECHIP" Microarray Core is operated under the supervision of S. Steven Potter, PhD. The GENECHIP" microarrays allow the simultaneous analysis of expression levels of tens of thousands of genes. This technology is becoming increasingly important in the study of developmental and disease processes. Received RNA samples are used to make biotinylated RNA for GENECHIP" microarray hybridization through a series of reactions involving cDNA synthesis, in vitro transcription, sample clean up and quantitation, spiking with control RNAs, and making to 1X hybridization solution. Each labeled RNA can be sequentially hybridized to at least four GENECHIP" probe arrays.
For a capsule summary of all the cores, visit our core summary page.
