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Movie 1. PGCs at E9.0. The trunk region of an E9.0 Oct4?PE:GFP embryo was dissected, cultured and filmed for 12 hrs. PGCs (green) can be seen moving around and between the cells of the hindgut. At this stage they are confined within the hindgut and do not exhibit directional movement with respect to the A/P or D/V axes. |
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Movie 2. PGCs at E9.5. A transverse slice was cut from the trunk of an E9.5 Oct4?PE:GFP embryo, cultured and filmed for 12 hrs. PGCs emerge from the dorsal most aspect of the hindgut, split into two streams and migrate towards the positions of the genital ridges. |
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Movie 3. PGCs at E10.5. A transverse slice was cut from the trunk of an E10.5 Oct4?PE:GFP embryo, cultured and filmed for 12 hrs. PGCs continue to migrate towards the positions of the genital ridges. At this stage they are slowing down and beginning to form clusters. Most germ cells left on the midline and in the hindgut mesentery fragment and die. |
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Movie 4A. SDF1 confuses PGC migration. A slice dissected from an E9.5 Oct4?PE:GFP embryo, treated with 1.6 µg/ml SDF1, and filmed for 7 hrs. |
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Movie 4B. Control slice for SDF1 treatment. An E9.5 slice was cultured in the absence of SDF. This slice was prepared and filmed simultaneously with the slice shown in 4A. |
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Movie 5A. LPA confuses PGC migration. An E9.5 slice cultured in the presence of 5 µM lysophosphatidic acid and filmed for 12 hrs. |
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Movie 5B. Control slice for LPA treatment. An E9.5 slice cultured in the presence of 5 µM phosphatidic acid. This slice was prepared and filmed simultaneously with the slice shown in 5A. |
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Movie 6A. DMS treatment blocks PGC clustering. An E9.5 slice cultured in the presence of 25 µM dimethylsphingosine and filmed for 12 hrs. DMS stock was brought up in methanol and diluted into medium for the culture experiment. |
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Movie 6B. Control slice for DMS treatment. An E9.5 slice cultured in the presence of 0.5 % methanol. This slice was prepared and filmed simultaneously with the slice shown in 6A. |
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Germ Cell E7.25 Movie Half of an E7.25 Stella-GFP embryo was dissected, cultured and filmed for about 3 hrs. |
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Germ Cell E7.5 Movie A Half of an E7.5 Stella-GFP embryo was dissected, cultured and filmed for about 12 hrs. |
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Germ Cell E7.5 Movie B Half of an E7.5 Stella-GFP embryo was dissected, cultured and filmed for about 12 hrs. |
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Germ Cell Ack2 Movie Half of an E7.5 Stella-GFP embryo was dissected, cultured with 10μg/ml Ack2 blocking antibody and filmed for about 6 hrs. |
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Germ Cell Control For Ack2 Treatment Movie Half of an E7.5 Stella-GFP embryo was dissected, cultured and filmed for about 6 hrs. This movie was prepared and filmed simultaneously with movie 3A. |
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E7.5 Steel WT Embryo Movie Half of an E7.5 Stella-GFP/Steel +/+ embryo was dissected, cultured and filmed for about 7 hrs. |
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E7.5 Steel Null Embryo Movie Half of an E7.5 Stella-GFP/Steel-/- embryo was dissected, cultured and filmed for about 7 hrs. |
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E7.5 Steel Heterozygous Embryo Movie Half of an E7.5 Stella-GFP/Steel+/- embryo was dissected, cultured and filmed for about 7 hrs. |
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E10.5 Embryo MovieA transverse slice was cut from the trunk of an E10.5 Oct4PE:GFP embryo, cultured and filmed for 12 hrs. E7.25 movie. |
