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Wylie-Heasman Lab

Grant Information Movies

Mouse Germ Cell Grant Movies

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Germ Cell E7.25 MovieHalf of an E7.25 Stella-GFP embryo was dissected, cultured and filmed for about 3 hrs.
Germ Cell E7.5 Movie A
Half of an E7.5 Stella-GFP embryo was dissected, cultured and filmed for about 12 hrs.
Germ Cell E7.5 Movie B Half of an E7.5 Stella-GFP embryo was dissected, cultured and filmed for about 12 hrs.
Germ Cell Ack2 MovieHalf of an E7.5 Stella-GFP embryo was dissected, cultured with 10μg/ml Ack2 blocking antibody and filmed for about 6 hrs.
Germ Cell Control For Ack2 Treatment MovieHalf of an E7.5 Stella-GFP embryo was dissected, cultured and filmed for about 6 hrs. This movie was prepared and filmed simultaneously with movie 3A.

 

E7.5 Steel WT Embryo Movie
Half of an E7.5 Stella-GFP/Steel+/+ embryo was dissected, cultured and filmed for about 7 hrs. 
E7.5 Steel Null Embryo Movie
 Half of an E7.5 Stella-GFP/Steel-/- embryo was dissected, cultured and filmed for about 7 hrs.



Xenopus Actin Grant Movies

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The time-lapse movie below shows two sibling Xenopus leavis embryos undergoing neurulation. The embryo in left is an uninjected control, the embryo in right has been depleted of N-cadherin protein in the neural plate using an antisense morpholino. In the N-cadherin depleted embryo, the neural plate fails to undergo invagination and remains open. Due to the pushing forces applied by the unaffected non-neural ectoderm, a distinct lip like structure appears surrounding the N-cadherin depleted neural plate.
N-cad MO neurulation