Developmental Biology

Rashmi S. Hegde, PhD

Title

Professor, Cincininnati Children's Hospital Medical Center

Appointment

Professor, University of Cincinnati College of Medicine

Email

rashmi.hegde@chmcc.org

Phone

513-636-5947

Fax

513-636-6772

Bio

Rashmi Hegde received her PhD in Medicinal Chemistry from the University of Pittsburgh in 1989. She received post-doctoral training in the Department of Molecular Bioophysics and Biochemistry at Yale University, where she was a fellow of the National Cancer Center. She was appointed Assistant Professor of Biochemistry at New York University School of Medicine and the Skirball Institute of Biomolecular Medicine in 1994. She is presently a full Professor in the Department of Pediatrics, University of Cincinnati College of Medicine and the Division of Developmental Biology at Childrens Hospital Research Foundation.

Credentials

PhD: University of Pittsburgh, Pittsburgh, PA, 1989

Post-doctoral Fellowship: Yale University, 1989-1994

Assistant Professor: New York University School of Medicine, Skirball Institute, 1994-2000

Associate Professor: (2001 - 2007) Cincinnati Children's Hospital Medical Center

Professor (2008 - present): Cincinnati Children's Hospital Medical Center

Awards and Honors

1993 National Cancer Center post-doctoral fellowship
1994 American Cancer Society Institutional Research Grant
1995 Whitehead Presidential Research Fellowship for Junior Investigators
1999 Dean's Recognition Citation for Excellence in Medical Education, NYU Med. Center

Research

Visit the Hegde Lab Site.

Molecular mechanisms underlying early stages in embryonic organ development, molecular basis of developmental defects and cancer, protein-DNA interactions, X-ray crystallography

Research Grants and Contracts

Mechanism of Action of Retinal Determination Proteins
Type: RO1 (2004 - 2009) EY014648
Role on Project: PI

DNA Target Selection by Papillomavirus E2 Proteins
Crystallographic studies of the papillomavirus E2 proteins and their complexes with DNA.
Type: R01 (1996-2006) CA66964
Role on Project: PI

Indirect Readout Mediation of Protein-DNA Interactions
Type: R01 (2001-2005) GM63582
Role on Project: Co-PI

Publications, Most Recent

Rashmi Hegde's publications as listed by PubMed

Trompette A., Divanovic S., Visintin A., Madan R., Blanchard C., Hegde R.S., Wills-Karp M., Gioannini T.L., Weiss J.P., Karp C.L. (2009) Functional mimicry between the major house dust mite allergen, Der p 2, and the TLR4 complex protein, MD-2.Nature, 457(7229):585-8.

Tan M., Xia M., Chen Y., Bu W., Hegde R.S. Meller J., Li X., Jiang X. (2009) Conservation of carbohydrate binding interfaces – Evidence of human HBGA selection in Norovirus evolution. PLoS ONE, in press.

Morrelae R., Fernandez-Fuentes N., Chen Z., Hegde R.S., Butsch-Kovacis M., Wells S.I., (2009) Insights from transcriptional profiling of human papillomavirus infection and associated carcinogenesis, review in Molecular Biology of Tumor Virus Gene Products (in press).

Blakaj D.M., Fernandez-Fuentes N., Chen Z., Hegde R.S., Fiser A., Burk R.D., Brenowitz M. (2009) Evolutionary, structural and electrostatic relationships among the papillomavirus E2 protein DNA binding domains.Frontiers in Bioscience, 14:900-17.

Bu W., Mamedova A., Xia M., Tan M., Jiang J., Hegde R.S. (2008) Structural basis for the receptor binding specificity of the Norwalk virus.J. Virology 82(11):5340-7.

Hu S.H., Mamedova A., Hegde R.S. (2008) DNA binding and regulation mechanisms of the SIX family of retinal determination proteins.Biochemistry, 47:3586-3594.

Liu, Y., Henry, G.D., Hegde, R.S., Baleja J.D. (2007) Solution structure of the hDlg/SAP97 PDZ2 domain and its mechanism of interaction with HPV-18 papillomavirus E6 protein.Biochemistry 46(38):10864-74.

Hegde R.S. (2006) Papillomavirus proteins and their potential as drug design targets.Future Virology, 1(6):795-810.

Rayapureddi J.P. and Hegde R.S. (2006) Branchio-oto-renal syndrome associated mutations in Eyes Absent 1 result in loss of phosphatase activity.FEBS Letters 580(16):3853-3859.

Liou B., Kazmierczek A., Zhang M., Scott C.R., Hegde R.S., Grabowski G. (2006) Analyses of variant Acid -Glucosidases: Effects of Gaucher disease mutations.Journal of Biological Chemistry 281(7):4242-53.

Blakaj D.M., Kattamuri C., Khrapunov S., Hegde R.S., Brenowitz M. (2006) Indirect readout of DNA sequence by papillomavirus E2 proteins depends upon cation uptake.Journal of Molecular Biology. 358(1):224-40.

Seufert D.W., Hegde R.S., Nekkalapudi S., Kelly L.E., El-Hodiri H.M. (2005) Expression of a novel Ski-like gene in Xenopus Development.Gene Expression Patterns 6(1):22-8.

Rayapureddi J.P., Kattamuri C., Chan F.H., Hegde R.S. (2005) Characterization of a plant, tyrosine-specific phosphatase of the aspartyl class.Biochemistry 44(2):751-8.

Tan M., Hegde R.S., Jiang J. (2004) The P domain of norovirus capsid protein forms a dimer and binds to histo-blood group antigen receptor.Journal of Virology 78(12):6233-42.

Blakaj D.M., Khrapunov S., Kattamuri C., Hegde R.S., Brenowitz M. (2004) Assessing the contribution of DNA structure and flexibility to DNA sequence specific recognition by papillomavirus E2 proteins.Biophysical Journal 86(1):590A-590A Part 2, Suppl. S.

Wilson J., Malakhova M., Zhang R.G., Joachimiak A., Hegde R.S. (2004) Crystal structure of the Dachshund-homology domain of human SKI.Structure 12(5):785-792.

Zhang Y., Xi A., Hegde R.S., Shakked Z., Crothers D.M. (2004) Predicting indirect readout effects in protein-DNA interactions.Proceedings of the National Academy of Sciences 101(22):8337-41.

Rayapureddi J.P., Kattamuri C., Steinmetz B.D., Frankfort B.J., Ostrin E.J., Mardon G., Hegde R.S. (2003) Eyes Absent represents a class of protein tyrosine phosphatases. Nature 426(6964):295-298 (News and Views, Nature (2003) 426(6964):238-9).

Kim S.S., Braunstein S.E., Zhang R.G., Joachimiak, A., Cvekl A., Hegde R.S., (2002) Structure of the retinal determination protein Dachshund reveals a DNA binding motif. Structure 10:787-795.

Hegde R.S., The papillomavirus E2 proteins: Structure, Function and Biology. (2002) Annual Reviews of Biophysics and Biomolecular Structure 31:343-360.

Kim S.S., Tam J.K., Wang A.F., and Hegde, R.S. (2000) The structural basis of DNA target discrimination by papillomavirus E2 proteins.Journal of Biological Chemistry 275:31245-31254.

Teaching

Join members of the Visual Systems Research Group for the The Great Lakes and Abrahamson Pediatric Eye Institute Vision Research Conference, November 11-12, 2005. Learn more about this conference here.

Special Interests

Research in the Hegde Laboratory is aimed at understanding the structural basis for specificity in macromolecular interactions. Currently the two main areas of interest in the laboratory are: