Developmental Biology

Yutaka Yoshida, PhD

Appointment

Assistant Professor

Email

yutaka.yoshida@cchmc.org

Phone

513-803-0943

Credentials

Postdoctoral fellow: Columbia University, 2002-2007; PI: Dr. Thomas M. Jessell.
Postdoctoral fellow: University of Tokyo, 1999-2002; PI: Dr. Tadashi Yamamoto.
PhD: University of Tokyo, 1999; PI: Dr. Tadashi Yamamoto.
BS: Keio University, 1994

Awards and Honors

Long Term Fellowship from Human Frontier Science Program Organization (HFSPO), 2003-2006

American Association for Cancer Research-ITOEN Young Investigator Award 1998

Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists (PD for postdoc)-1999-2002

Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists (DC1-for graduate student)-1996-1999

Research

The fidelity with which neural circuits are assembled during development has a critical influence on the later function of these circuits during defined behaviors. Our lab is interested in the molecular mechanisms that direct the assembly of neural circuits in the developing spinal cord.

Factors that control initial axonal trajectories are relatively well understood, but it remains unclear how axons establish their distinct termination zones and form selective synaptic connections. These remain important problems in neurobiology.

The spinal neural circuit is an excellent model system to approach this problem because of its relative simplicity and its solid base of previous anatomical and electrophysiological analyses. Our lab uses many techniques including molecular biology, mouse genetics, biochemistry, and electrophysiology to address these questions.

Research Grants and Contracts

R01NS065048 (04/01/2009 – 03/31/2014)

PI: Yutaka Yoshida
Regulation of Sensory-Motor Connectivity by Semaphorin-Plexin Signaling
Total direct cost: $1,093,750

BASIL O'CONNOR STARTER SCHOLAR RESEARCH AWARD (March of Dimes) (2/1/2009-1/31/2011)
PI: Yutaka Yoshida
Role of Sema3EplexinD1 signaling in synaptic specificity in the developing mouse spinal cord
Total direct cost: $136,354

Publications, Most Recent

Pecho-Vrieseling E., Sigrist M., Yoshida Y., Jessell T.M., and Arber S (2009).
Specificity of Monosynaptic Sensory-Motor Connections Encoded by Sema3e-PlexinD1.Nature, 459, 842-6.

Zhang Y., Degenhardt K.R., Lu M.M., Bennett J., Yoshida Y., and Epstein J.D. (2009). Endothelial inactivation of PlexinD1 results in congenital heart, vascular and skeletal defects.Developmental Biology, 325, 82-93

Yoneda M, Suzuki T, Nakamura T, Ajima R, Yoshida Y, Kakuta S, Sudo K, Iwakura Y, Shibutani M, Mitsumori K, Yokota J, Yamamoto T (2009). Deficiency of antiproliferative family protein Ana correlates with development of lung adenocarcinoma.Cancer Sci, 100, 225-32.

Arlotta P., Molyneaux B.J., Jabaudon D., Yoshida Y., and Macklis J.D. (2008). Ctip2 controls the differentiation of medium spiny neurons and the establishment of the cellular architecture of the striatum. J Neurosci. 28, 622-32.

Kanda T., Yoshida Y., Izu Y., Nifuji A., Ezuna Y., Nakashima K., and Noda M. (2007). PlexinD1 deficiency induces defects in axial skeletal morphogenesis. J Cell Biochem. 101, 1329-37.

Chauvet S., Cohen S., Yoshida Y., Fekrane L., Livet J., Gayet O., Segu L., Buhot M.C., Jessell T.M., Henderson C.E., and Mann F. (2007). Gating of sema3E/plexinD1 signaling by neuropilin-1 switches axonal repulsion to attraction during brain development. Neuron 56, 807-22.

Yoshida Y., Han B., Mendelsohn M., and Jessell T.M. (2006). PlexinA1 signaling directs the segregation of proprioceptive sensory axons in the developing spinal cord. Neuron 52, 775-88.

Wilson J.M., Hartley R., Maxwell D.J., Todd A.J., Lieberam I., Kaltschmidt J.A., Yoshida Y., Jessell T.M., Brownstone R.M. (2005). Conditional rhythmicity of ventral spinal interneurons defined by expression of the Hb9 homeodomain protein. J Neurosci. 25, 5710-9.

Gu C.,* Yoshida Y.,*, Livet J., Reimert D.V., Mann F., Merte J., Henderson C.E., Jessell T.M., Kolodkin A.L., Ginty D.D. (2005). Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins. Science 307, 265-8. (* : equal contribution)

Kawamura-Tsuzuku J., Suzuki T., Yoshida Y., Yamamoto T. (2004). Nuclear localization of Tob is important for regulation of its antiproliferative activity. Oncogene 23, 6630-8.

Nakamura T., Yao R., Ogawa T., Suzuki T., Ito C., Tsunekawa N., Inoue K., Ajima R., Miyasaka T., Yoshida Y., Ogura A., Toshimori K., Noce T., Yamamoto T., and Noda T. (2004). Oligo-astheno-teratozoospermia in mice lacking Cnot7, a regulator of retinoid X receptor beta. Nat Genet. 36, 528-33.

Usui M., Yoshida Y., Tsuji K., Oikawa K., Miyazono K., Ishikawa I., Yamamoto T., Nifuji A., and Noda M. (2004). Tob deficiency superenhances osteoblastic activity after ovariectomy to block estrogen deficiency-induced osteoporosis. Proc Natl Acad Sci U S A. 101, 6653-8.

Yoshida Y., von Bubnoff A., Ikematsu N., Blitz IL., Tsuzuku JK., Yoshida EH., Umemori H., Miyazono K., Yamamoto T., and Cho KW. (2003). Tob proteins enhance inhibitory Smad-receptor interactions to repress BMP signaling. Mech Dev. 120, 629-37.

Yoshida Y., Nakamura T., Komoda M., Satoh H., Suzuki T., Tsuzuku JK., Miyasaka T., Yoshida EH., Umemori H., Kunisaki RK., Tani K., Ishii S,. Mori S., Suganuma M., Noda T., and Yamamoto T. (2003). Mice lacking a transcriptional corepressor Tob are predisposed to cancer.Genes Dev. 17, 1201-6.

Suzuki T., K-Tsuzuku J., Ajima R., Nakamura T., Yoshida Y., and Yamamoto T. (2002). Phosphorylation of three regulatory serines of Tob by Erk1 and Erk2 is required for Ras-mediated cell proliferation and transformation.Genes Dev. 16, 1356-70.

Yoshida, Y., Tanakam S., Umemori, H., Minowa, O., Usui, M., Ikematsu, N., Hosoda, E., Imamura, T., Kuno, J., Yamashita, T., Miyazono, K., Noda, M., Noda, T., and Yamamoto, T. (2000). Negative regulation of BMP/Smad signaling by Tob in osteoblasts.Cell 103, 1085-97.

Presentations, Most Recent

2009
Wright State University, Dayton

2008
Harvard University, Boston

2007
Albert Einstein College of Medicine, New York
Yale University, Connecticut
Hunter College, New York
Brandeis University, Massachusetts
Cincinnati Children's Meical Center, Cincinnati
Memorial Sloan-Kettering Cancer Center, New York
Keio University, Japan
Tohoku University, Japan
Kyoto University, Japan
Osaka Bioscience Institute, Japan
Osaka University, Japan
University of Tokyo, Japan
Tokyo Medical and Dental University, Japan
RIKEN Brain Science Institute (BSI), Japan

2006
The Institut de recherches cliniques de Montréal (IRCM), Canada
Harvard University, Boston

2005

University of Tokyo, Japan

Tokyo Medical and dental University, Japan