Mulloy Laboratory Research Interests
Research in the Mulloy Laboratory at Cincinnati Children's Hospital Medical Center focuses on using human hematopoietic stem cells to model leukemia. The prevailing theory is that acute myeloid leukemia is a stem cell disease. We believe the technology and expertise are now available that permit us to use primary human cells, and specifically the human hematopoietic stem cell, to set up relevant model systems to dissect the process of human AML. As a proof of principal, we are expressing the fusion protein, AML1-ETO, in human CD34+ stem cells by retroviral transduction and characterizing the genetic and functional alterations that occur due to expression of this oncogene.
Our studies of human leukemogenesis ask the following questions:
- What are the effects of AML1-ETO expression on the self-renewal and differentiation properties of human HSC?
- Can we develop relevant in vivo models of human leukemia using transplantation of these cells in the immunocompromised NOD/SCID or NOD/SCID/b2M mouse?
- What are the target genes of AML1-ETO that are essential for the leukemic phenotype?
For additional information, please contact Dr. James Mulloy, Division of Experimental Hematology, at 513-636-1844. Dr. Mulloy's office can be found in room S7.603.
