Hematology / Oncology

Kejian Zhang, MD, MBA

Title

Director, Molecular Genetics Laboratory

Appointment

Assistant Professor of Pediatrics, University of Cincinnati College of Medicine

Email

kejian.zhang@cchmc.org

Phone

513-636-0121

Fax

513-636-2261

Credentials

MD: Tianjin Medical University, Tianjin, China, 1993

MBA: University of Cincinnati, College of Business Administration, 2001

Residency: Gong'an Hospital, Tianjin, China, 1993-1995

Fellowship: Clinical Molecular Genetics Fellow, Division of Human Genetics, Cincinnati Children's Hospital, 2002-2004

Certification: Clinical Molecular Genetics, certified by the American Board of Medical Genetics in 2005

Awards and Honors

2009: Patent Diagnostic Assay for Wiskott-Aldrich Syndrome and Genetically Associated Disorders was granted on September 01, 2009 by the United States Patent and Trade Mark Office. Patent number: US 7, 582, 738B2

2009: Second place in research category at 2009 Nurse Poster Day poster presentation “Pharmacogenetic Test Results and Aggressive Behavior Treatment Response In Hospitalized Pediatric Psychiatric Patients”

2008: First place for Capstone Poster Session in 2008 Summer Undergraduate Research Program (SURP) at Cincinnati Children’s Hospital Medical Center for presenting “Race/Ethnicity and Drug Metabolism: Genotype and Phenotype Analysis of CYP2D6 and CYP2C19”.

2007:Received the C. Nezelof Awardby the Histiocyte Societyin September 2007 at 23rd Annual Meeting in Cambridge, United Kingdom. Presented “Genetic Profiles of Munc13-4 in Patients with Familial Hemophagocytic Lymphohistiocytosis in North America” at the presidential symposium.

2005: Outstanding Achievement in Promoting the profession of Laboratory Medicine and Research Awarded by the Division and Program in Human Genetics of Cincinnati Children’s Hospital Medical Center

2002-2004: Established the International Molecular Diagnostic Reference Center for Inherited Immunodeficiency Diseases at Molecular Genetics Laboratory, CCHMC

Research

Molecular defects and molecular diagnosis of primary immunodeficiency diseases.

A study entitled "Retrospective Analysis of Correlation between Immunologic Phenotype and Genotype in Patients with Hemophagocytic Lymphohistiocytosis (HLH)" CCHMC IRB #06-05-45 (6/5/2006-6/5/2007)
"Macrophage Activation Syndrome Biomarkers in Systemic JRA" is funded by CCHMC TRI grant 2007-2008.

Genetic aspects of predictive personalized medicine, eg., Pharmarcogenetics.

"Pharmacogenetics of Antiepileptic Drug Efficacy, Metabolism and Toxicity in Children with Epilepsy" (8/8/2003-2008)
"Open-Label Study of the Safety, Tolerability and Efficacy of Individualized Selective Serotonin Reuptake Inhibitor Dosing During Acute Treatment for Adolescents with Depression Based on Cytochrome P450 2D6 and 2C19 Genotype" (8/3/2006-2007)
"Pharmacogenetics of Mycophenolic Acid in Kidney Transplant Patients" (on-going). CHMC#06-06-44
"Effect of genetic pharmacology service's Psychiatry panel: retrospective chart review" ( Study period Jan 1, 2000 to Sep 30, 2005)
"Biomarkers for Patient-tailored Therapies in Pediatric Lupus Nephritis using Mycophenolic Acid", this is funded by CCHMC Translational Research Initiative (TRI) grant 2007-2008

Other research activities:

"Fanconi Anemia SeqChip" development. Serves as consultant in design and implement a comprehensive "Fanconi Anemia SeqChip" consisting of a single Affymetrix resequencing chip that will facilitate rapid, reproducible and cost-effective diagnostic sequence analysis of FA-causing genes as well as distinction of FA from other chromosome breakage/bone marrow failure syndromes.

Research Grants and Contracts

Macrophage Activation Syndrome Biomarkers in Systemic Juvenile Idiopathic Arthritis
Sponsor: NIH P60, NIAMS Multidisciplinary Clinical Research Centers.
PI: David Glass M.D., Division of Rheumatology
Co-investigator: Kejian Zhang MD, MBA, Alexi Grom M.D., Jack Bleesing, MD, PhD, and Daniel Lovell, MD, MPH
Date of submission: Sep01, 2007
Status: funded 08/01/07-12/01/12

Molecular Diagnosis and Biomarkers for Esophagitis
Sponsor: CCHMC TRI
PI: Marc Rothenberg
Co-Investigator: Kejian Zhang
Date of Submission: November 25, 2008
Status: Funded

Personalizing Perioperative Analgesia for Adolescents Undergoing Major Spine Surgeries.
Sponsor: CCHMC outcome research Grant
PI: Senthilkumar Sadhasivam MD
Co-Investigator: Kejian Zhang
Date of submission: November 2008
Funding period: July 2009- June 2011
Status: Funded, $60,000 per year for two years

Pediatric Warfarin Dosing Using Genetic & Clinical Data
Sponsor: University of Cincinnati CCTST Pilot Grant Program, T1
PI: Shannon Saldana
Lab Manager: Kejian Zhang
Date of Submission: August 1, 2009
Status: Funded

“Fanconi Anemia SeqChip” development.
PI: Michael Jansen, PhD, Division of Experimental Hematology
Consultant: Kejian Zhang, MD, MBA
Sponsor: CCHMC TRI
Status: Funded, 2006-2007
Objective of this grant is to design and implement a comprehensive “Fanconi Anemia SeqChip” consisting of a single Affymetrix resequencing chip that will facilitate rapid, reproducible and cost-effective diagnostic sequence analysis of FA-causing genes as well as distinction of FA from other chromosome breakage/bone marrow failure syndromes.

Open-Label Study of the Safety, Tolerability and Efficacy of Individualized Selective Serotonin Reuptake Inhibitor Dosing During Acute Treatment for Adolescents with Depression Based on Cytochrome P450 2D6 and 2C19 Genotype.
PI: Sanjeev Pathak, M.D.
Co-investigators: Tracy A. Glauser, M.D., Richard Wenstrup, M.D., Kejian Zhang         MD, MBA, Alexander A. Vinks, Ph.D., Todd Nick, Ph.D., Michael T. Sorter,    M.D., Arman Danielyan
DATE: August 3, 2006.
Sponsor: CCHMC and Division of Human Genetics
Status: Funded

Pharmacogenetics of Antiepileptic Drug Efficacy, Metabolism and Toxicity in Children with Epilepsy
Date: August 8, 2003-2008
Principal Investigator: Tracy Glauser, M.D.
Co-Investigator: Alexander Vinks, PharmD, PhD, Diego Morita, M.D., (Richard          Wenstrup M.D.), Kejian Zhang MD, MBA, Teresa Smolarek, Ph.D., Lisa Martin,    Ph.D., Stephen Fordyce
Sponsor: Epilepsy Foundation of American
Status: Funded

Pharmacogenetics of Mycophenolic Acid in Kidney Transplant Patients
Principal Investigator: Alexander A. Vinks, PharmD, PhD, FCP
Pediatric Pharmacology Research Unit
Co-Investigators: Jens Goebel, MD., Kejian Zhang MD, MBA, and Todd Nick, Ph.D.
Sponsor: Translational Research Initiative (TRI),
Pediatric Pharmacology Research Unit (PPRU)
Date of Submission: June 22, 2006
Status: Funded

Macrophage Activation Syndrome Biomarkers in Systemic JRA
Principal Investigator: Alexi Grom MD
Division of Reumatology
Co-Investigators: Kejian Zhang MD, MBA
Sponsor: Translational Research Initiative (TRI),
Date of Submission: December, 2006
Status: Funded
Period: July01, 2007-June 30, 2008

Biomarkers for Patient-tailored Therapies in Pediatric Lupus Nephritis using Mycophenolic Acid
Sponsor: CCHMC TRI grant 2007-2008,
Principal Investigator: Hermine Brunner MD
Division of Reumatology
Co-Investigators: Kejian Zhang MD, MBA
Sponsor: Translational Research Initiative (TRI),
Date of Submission: June 22, 2006
Status: Funded

Molecular Diagnosis of Fanconi Anemia
Principal Investigator: Michael Jordan
Division of Experimental Hematology
Co-Investigator: Kejian Zhang MD, MBA
Sponsor: CCHMC Trustee
Date of Submission: November 01, 2006
Status: Funded

Primary Immunodeficiency International Conference
Sponsor: NIH NACID
PI: Lisa Filipovich MD, Professor of Pediatrics
Co-PI: Kejian Zhang MD, MBA and Jack Bleesing MD
Date of Submission: November 2008
Status: Funded. Host the PIDD meeting in November 2008, and more than 60 individuals from 15 different countries attended this meeting.

Pharmacogenetics of IMPDH in Kidney Transplant Patients
Sponsor: CCHMC TRI
PI: Alexander A. Vinks PharmD. Professor of Pediatrics and Pharmacology (Adj.)
Director, Pediatric Pharmacology Research Unit
Co-investigator: Kejian Zhang MD, MBA
Date of submission: Dec07, 2007
Status: $ 100,000 Funded
Period: July01, 2008-June30, 2009

Development of a cardiomyopathy resequencing chip for pediatric patients
Sponsor: CCHMC TRI
PI: Stephanie Ware M.D., PhD. Assistant Professor of Pediatrics
Division of Cardiology and Human Genetics
Collaborator: Kejian Zhang MD, MBA
Date of submission: Dec07, 2007
Status: $ 93,250 Funded
Period: July01, 2008-June30, 2009

Improving Acute Pediatric Pain Management Using Neurogenomics
Sponsor: CCHMC TRI
PI: John McAliff, MD, MBA. Professor of Clinical Anesthesia and Pediatrics
Department of Anesthesiology
Co-investigator: Kejian Zhang MD, MBA
Date of submission: Dec07, 2007
Status: $76,400 Funded
Period: July01, 2008-June30, 2009

CYP2D6 Pharmacogenetics in Risperidone-Treated Children
Sponsor: CCHMC TRI
PI: Saldaña, Shannon N., PharmD, MS. Instructor of Pediatrics
Pharmacy and Pediatric Pharmacology Research Unit
Co-investigator: Kejian Zhang MD, MBA
Date of submission: Dec 07, 2007
Status: $75,669 Funded
Period: July 01, 2008-June 30, 2009

Development of a cardiomyopathy resequencing chip for pediatric patients
Sponsor: Mitochondrial Research Foundation
PI: Stephanie Ware M.D., PhD. Assistant Professor of Pediatrics
Division of Cardiology and Human Genetics
Collaborator: Kejian Zhang MD, MBA
Date of submission: February 2008
Status: Funded
Period: July 01, 2008-June 30, 2009

Pursuing Perfection in Pediatric Therapeutics
Sponsor: 1U18HS016957
PI:   Lannon (PI) Professor of Pediatrics, AHRQ Centers for Education in Research and Therapeutics (CERT)
Collaborator: Michael Seid MD, Kejian Zhang MD, MBA
Date of submission: February 2008
Status: Funded $995,750 / $2,717,142
Period: 09/01/07-08/31/11

Publications, Most Recent

Andrew L. Snow, Rebecca A. Marsh, Scott M. Krummey, Philip Roehr, Lisa R.Young, Kejian Zhang, Jack van Hoff, Deepali Dhar, Kim E. Nichols, Alexandra H.Filipovich, Helen C. Su, Jack J. Bleesing, and Michael J. Lenardo. SAP Deficiency Impair NTB-A-Depandent restimulation induced apoptosis of T cells from X-linked lymphoproliferative disease patients" (reference 39518- RG-RV-2). Accepted to The Journal of Clinical Investigation, July 2009.

Nancy Leslie, Brad Tinkle, Kerry Shooner, Arnold W. Strauss, and Kejian Zhang GeneReview: Very Long Chain Acyl-CoenzymeA Dehydrogenase Deficiency, submitted for review(2009). GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle, May 2009. Available at www.genetests.org

Marsh MA, Villanueva J, Kim MO, Zhang K, Marmer D, Risma KA, Jordan MB, Blessing JJ, Filipovich AH. Patients with X-linked Lymphoproliferative Disease Due to BIRC4 mutation have normal invariant natural Killer T-cell populations. Clinical Immunology, 2009 Apr22 (Epub ahead of print)

Sumegi J, Johnson J, Filipovich A, Rebecca Marsh, and Zhang K (updated March 2009) Lymphoproliferative Disease, X-Linked in: GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle. 1997-2006. Available at www.genetests.org.

Pestian J, Spencer M, Matykiewicz P, Zhang K , Vinks S, Glauser T: Personalizing Drug Selection Using Advanced Clinical Decision Support, Biomedical Informatics Insights, March 2009 2:19-19

Rebecca A. Marsh, Joyce Villanueva, Kejian Zhang, Andrew Snow, Helen C. Su, Lisa Madden, Dan Marmer, Michael Jordan, Kimberly Risma, Alexandra Filipovich, Jack J. Bleesing. Rapid Flow Cytometric Identification of Patients with X-linked Lymphoproliferative Disease Due to XIAP Deficiency, Cytometry B. Clin Cytom, Accepted 28 January 2009, Published online in Wiley InterScience (www.interscience.wiley. com), DOI: 10.1002/cyto.b.20473

Jack Bleesing, Kejian Zhang, Judy Johnson: Autoimmune Lymphoproliferative Syndrome, Sumbitted to Gene Review, Feb 2009. GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle, 1997-2009. Available at: www.genetests.org

Cindy Prows, Todd Nick, Shannon Saldana, Sanjeev Pathak, Kejian Zhang, Chunyan Liu, Zachary Daniels, Alexander Vinks, Tracy Glauser: Drug Metabolizing Enzyme Genotypes and Aggressive Behavior Treatment Response In Hospitalized Pediatric Psychiartric Patients, in Journal of Child and Adolescent Psychopharmacology, 2009 Aug;19(4):385-94.

Susan E. Prausa, Tsuyoshi Fukuda, Denise Maseck, Kristi L. Curtsinger, Chunyan Liu, Kejian Zhang, Todd G. Nick, Jens Goebel, and Alexander A. Vinks: UGT Genotype May Contribute to Adverse Events of Mycophenolate Mofetil in Pediatric Kidney Transplant Recipients. Clinical Pharmacology and Therapeutics. Advance online publication 18 February 2009.

Kejian Zhang, Jennifer Biroschak, David N. Glass, Susan Thompson, Terri Finkel, Murray H. Passo, Alexandra Filipovich and Alexei A. Grom. Macrophage Activation Syndrome in Systemic Juvenile Idiopathic Arthritis is Associated with MUNC13-4 Gene Polymorphisms. Arthritis and Rheumatism, 2008, in press

Friedlander SL, Dooms KT, Voss CY, Agger WA, Zhang K, Bleesing J, Filipovich AH, Seroogy CM, Adolescent Presentation of X-linked Lymphoproliferative Disease, Annals of Allergy, Asthma & Immunology, 2008 Apr;100(4):398-400

Bleesing J, Johnson J, Zhang K (Revision July 2007) Autoimmune Lymphoproliferative Syndrome in: GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle, 1997-2006. Available at www.genetests.org

Johnson J, Filipovich A, Zhang K (May 2007) X-linked Hyper IgM Syndrome in: GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle, 1997-2007. Available at www.genetests.org

Filipovich A, Johnson J, Zhang K (updated April 2007) WAS-Related Disorders in: GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle. 1997-2007. Available at www.genetests.org

Zhang K, Filipovich AH, Johnson J (Revision March 2007) Hemophagocytic Lymphohistiocytosis, Familial. In: GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle, 1997-2007. Available at www.genetests.org.

Kejian Zhang, Judith A. Johnson, Jennifer Biroschak, Joyce Villanueva, Susan M. Lee, Jack J. Bleesing, K.A.Risma, Richard J. Wenstrup and Alexandra H. Filipovich. The A91V substitution in the Perforin 1 (PRF1) gene is found in patients with Familial Hemophagocytic Lymphohistiocytosis (FHL) in combination with other PRF1 or Munc13-4 mutations. International Journal of Immunogenetics (2007) 34,231-233

Bleesing J, Johnson J, Zhang K (September 2006) Autoimmune Lymphoproliferative Syndrome in: GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle, 1997-2006. Available at www.genetests.org

Lee SM, Sumegi J, Villanueva J, Tabata Y, Zhang K, Chakraborty R, Sheng X, Clementi R, de Saint Basile G, Filipovich AH. Patients of African ancestry with hemophagocytic lymphohistiocytosis share a common haplotype of PRF1 with a 50delT mutation. J Pediatr 2006 Jul;149(1):134-137

Sumegi J, Johnson J, Filipovich A, Zhang K (updated August 2006) Lymphoproliferative Disease, X-Linked in: GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle. 1997-2006. Available at www.genetests.org.

Zhang K, Filipovich AH, Johnson J (March 2006) Hemophagocytic Lymphohistiocytosis, Familial. In: GeneReviews at GeneTests: Medical Genetics Information Resource [database online]. Copyright, University of Washington, Seattle, 1997-2006. Available at www.genetests.org.

Presentations, Most Recent

Marsh RA, Jordan MB, Bleesing JJ, Zhang K, Filipovich AH. XIAP Deficiency: A Cause of Familial Hemophagocytic Lymphohistiocytosis Type V? is nominated for C. Nezelof Award by the 2009 Annual Meeting of Histiocyte Society.

Edita Freeman, Mehdi Keddache, Shannon Saldana, Cynthia Prows, Alexander A. Vinks, Tracy Glauser and Kejian Zhang. A Faster and More Sensitive Assay for CYP2D6 and CYP2C19 Genotyping. Present to annual meeting of American Molecular Pathology Kissimmee, Florida, 2009.

Kejian Zhang, Jaroslaw (Jarek) Meller, Jennifer Biroschak, Judith A. Johnson, Qian (Eric) Wei, Rebecca A. Marsh, Alexi Grom, Kimberly Risma, Jack Bleesing, and Alexandra H. Filipovich. Mutation Spectrums of PRF1, MUNC13-4 and STX11 genes in patients with in North America, Present at the annual meeting of American Society of Human Genetics, Honolulu, Hawaii, 2009

Kejian Zhang, Judith A. Johnson, Jennifer Biroschark, Noriko Satake, Rebecca Marsh and Alexandra H. Filipovich, Genetic Profiles of STX11 gene in Patients with Familial Hemophagocytic Lymphohistiocytosis in North America, presented at the 2009 Meeting of the American College of Medical Genetics, Tampa Florida, March 2008

Snow AL, Marsh RA, Krummey SK, Roehrs P, Zhang K, Filipovich AH, Su HC, Bleesing JJ, Lenardo MJ. SAP Augments Proximal T Cell Receptor Signal Strength Necessary for Restimulation-induced Apoptosis of Activated T Cells. Presented at the FOCIS meeting 2009.

Tinkle BT, Peters S, Zhang K, Leslie N (2008). Genetic testing of GAA: a prompt and reliable diagnosis of Pompe disease. Presented at the annual meeting of American Society of Human Genetics, Philadelphia, 2008

Liu C, Richardson B, Schalk A, Mourya R, Bezerra J, Zhang K. Implementation of JaundiceChip as a Clinical Diagnostic Tool. Will present at the annual meeting of American Society of Human Genetics, Philadelphia, 2008.

Zhang K, Biroschak J, Wei Q, Johnson JA, Bleesing J, Glass DN, Thompson S, Grom AA, Filipovich AH. A Unique Haplotype of the MUNC13-4 Gene Associated with Familial Hemophagocytic Lymphohistiocytosis and Macrophage Activation Syndrome in Systemic Juvenile Idiopathic Arthritis. Will present at XIIIth European Society of Immonodeficiencies meeting in 's-Hertogenbosch, Netherland, 2008.

Freeman E, Schalk A, Keddache M, Zhang K. Evaluation of A Commercially Available Product for Fragile X Testing in a Mid-size Clinical Laboratory. Will present at the annual meeting of American Molecular Pathology Grapevine, TX, 2008.

Establishing genetic testing for rare diseases. Invited presentation at Active Communication International (ACI) Conference on Successful Strategies for Genetic Testing in Boston, July 2008.

Cell Populations Are Not Decreased in the Majority of Patients with XIAP Deficiency. Presented at the 2008 Meeting of The Federation of Clinical Immunology Societies (FOCIS), Boston, USA.

Genetic defects in patients with X-linked Lymphoproliferative Syndrome in North American. Presented at the 2007 Meeting of the American Society of Human Genetics, San Diego, California, USA.

Genetic Profiles of Munc13-4 in Patients with Familial Hemophagocytic Lymphohistiocytosis (HLH) in North America. Presented at the 23rd Annual Meeting of the Histiocyte Society September 2007 at Cambridge, United Kingdom.

Profiles of Munc13-4 gene in Patients with Familial Hemophagocytic Lymphohistiocytosis and Macrophage Activation Syndrome. Presented at the 2007 Meeting of the American College of Medical Genetics, Nashville, Tennessee, March 2007.

Molecular mechanisms of Non-Mendelian Inheritance. Presented as a lecture at the genetic counseling program of 2006-2007 in the College of Medicine and University of Cincinnati.

Heterozygosity of A91V-PRF1 in patients with Familial Hemophagocytic Lymphohistiocytosis. Presented at the 2006 Congress of European Society of Immunodeficiencies, Budapest, Hungary.

Resent Advances in the Molecular Diagnoses of Inherited Immunodeficiencies: Implications for Genetic Counselors. Presented as an education breakout session at the 23rd Annual Education Conference by National Society of Genetic Counselors, Inc.

PRF1 and Munc13-4 Mutations in North American Patients with Familial Hemophagocytic Lymphohistiocytosis. Presented at the 2006 Meeting of the American Society of Human Genetics, New Orleans, LA, USA.

Characterization of herpsvirus saimiri-transformed cytotoxic T lymphocytes from patients in perforin or SAP/SH2D1A deficiency. Presented at the 2005 congress of European Society of Immunodeficiencies.

Professional Organization Memberships

American Society of Human Genetics

American College of Medical Genetics

American Society of Molecular Pathology

Public Population Project in Genomics (P3G)

Chinese Association of Medical Genetics

The International Histiocyte Society

Books

Thomas Burrow, Kejian Zhang and Gregory A. Grabowski, Chapter of Diseases of Genetic Origin for the fifth edition (2009) of Clinical Chemistry: Theory, Analysis, Correlation by Lawrence Kaplan and Amadeo Pesce, July 2009

Pestian J, Spencer M, Matykiewicz P, Zhang K, Vinks S, Glauser T, Personalizing Drug Selection Using Advanced Clinical Decision Support. 2009 Biomedical Informatics Insights, Volume 2; 1-11.