Claire A. Chougnet, PhD
Appointment
Associate Professor
Email
claire.chougnet@chmcc.org
Phone
513-636-8847
Fax
513-636-4278
Credentials
Diplôme de Pharmacien (DPharm.): Université Paris XI, Paris, France, 1980.
CES (French specialized degrees; clinical biologist): Immunology, Hematology, Bacteriology-Virology, Parasitology, 1984.
PhD: Université Paris V, 1991.
Awards and Honors
- 1985 Gold Medal, Pharmacy residency program, Toulouse, France.
- 1991 PhD award, University Paris V, France
- 1998 "Habilitation à diriger les recherches" (diploma allowing the supervision of research trainees), France
- 1999- 2000 Ad hoc reviewer for grant proposals: Swiss National Science Foundation
- 2001 Co-Organizer, Think Tank meeting "Antigen-presenting cells in HIV Pathogenesis and Therapy"
- 2002- 2005 Ad hoc reviewer for grant proposals: Ontario HIV Treatment Network
- 2006- 2011 Ad hoc member, and Member of NIH AIDS Immunology and Pathogenesis Study Section
- 2006 Co-Organizer, workshop "Regulatory T cells and HIV/AIDS"
- 2006 Member "Faculty of 1000 Biology" (Faculty: Immunology, Section: Immunity to Infections).
- 2007 Member of NIH AIDS Immunology and Pathogenesis Study Section.
Research
HIV/AIDS Pathogenesis
Immune dysfunction in aging
Ontogeny of immune system
Research Grants and Contracts
- NIH R01 DK065008
07/01/03 - 06/30/07
Dysfunction in biliary atresia - NIH R01 AI056927
01/01/05 - 12/31/09
CD40 Ligand dysregulation and HIV pathogenesis - NIH R21 AG025149
09/01/05 - 08/31/07
Immune suppression and regulatory T cells in aging - Dean Discovery Fund
08/01/05 - 12/31/05
Evaluation of the effect of low-dose IL-2 immunotherapy on the immune responses in HCV/HIV co-infected patients. - Abbott Laboratories (PI: C. Fichtenbaum; co-PI: C. Chougnet)
12/06/05 - 12/06/07
Immune reconstitution as a determinant of adverse effects to new antiretroviral therapy in persons with advanced HIV infection. - NIH R01 (PI: C. Chougnet)
8/1/06 - 7/31/11
Role of Regulatory T cells in HIV Pathogenesis - NIH R21 AG025149 (PI: C. Chougnet)
9/1/05 - 7/31/08
Immune suppression and regulatory T cells in aging - Abbott Laboratories (PI: C. Fichtenbaum; co-PI: C. Chougnet)
12/06/05 - 3/01/08
Immune reconstitution as a determinant of adverse effects to new antiretroviral therapy in persons with advanced HIV infection - NIH R01 DK 065008 (PI: J. Bezerra; co-PI: C. Chougnet)
8/1/03 - 12/31/11
Dysfunction in biliary atresia
Publications, Most Recent
- Chougnet C, Freitag C, Schito M, Thomas EK, Sher A, Shearer GM. (2001) In vivo CD40-CD154 (CD40 ligand) interaction induces integrated HIV expression by APC in an HIV-1-transgenic mouse model.J Immunol 166:3210-7.
- Freitag C, Chougnet C, Schito M, Near KA, Shearer GM, Li C, Langhorne J, Sher A. (2001) Malaria infection induces virus expression in human immunodeficiency virus transgenic mice by CD4 T cell-dependent immune activation.J Infect Dis 183:1260-8.
- Chougnet C, Jankelevich S, Fowke K, Liewehr D, Steinberg SM, Mueller BU, Pizzo PA, Yarchoan R, Shearer GM. (2001) Long-term protease inhibitor-containing therapy results in limited improvement in T cell function but not restoration of interleukin-12 production in pediatric patients with AIDS.J Infect Dis 184:201-5.
- Chougnet C, Shearer GM, Landay AL. (2002) The Role of Antigen-presenting Cells in HIV Pathogenesis.Curr Infect Dis Rep 4:266-271.
- Landay A, Chougnet C, Frankel SS, Veronese F, Shearer GM. (2002) Antigen-presenting cells in HIV pathogenesis and therapy: summary of the October 17-18, 2002, Think Tank meeting.Clin Immunol 103:243-8.
- Lu Y, Raviprakash K, Leao IC, Chikhlikar PR, Ewing D, Anwar A, Chougnet C, Murphy G, Hayes CG, August TJ, Marques ET, Jr. (2003) Dengue 2 PreM-E/LAMP chimera targeted to the MHC class II compartment elicits long-lasting neutralizing antibodies.Vaccine 21:2178-89.
- Chougnet C. (2003) Role of CD40 ligand dysregulation in HIV-associated dysfunction of antigen-presenting cells.J Leukoc Biol 74:702-9.
- Zhang R, Fichtenbaum CJ, Hildeman DA, Lifson JD, Chougnet C. (2004) CD40 ligand dysregulation in HIV infection: HIV glycoprotein 120 inhibits signaling cascades upstream of CD40 ligand transcription.J Immunol 172:2678-86.
- Herbeuval JP, Boasso A, Grivel JC, Hardy AW, Anderson SA, Dolan MJ, Chougnet C, Lifson JD, Shearer GM. (2005) TNF-related apoptosis-inducing ligand (TRAIL) in HIV-1-infected patients and its in vitro production by antigen-presenting cells.Blood 105:2458-64.
- Herbeuval JP, Grivel JC, Boasso A, Hardy AW, Chougnet C, Dolan MJ, Yagita H, Lifson JD, Shearer GM. (2005) CD4+ T-cell death induced by infectious and noninfectious HIV-1: role of type 1 interferon-dependent, TRAIL/DR5-mediated apoptosis.Blood 106:3524-31.
- Zhang R, Lifson JD, Chougnet C. (2006) Failure of HIV-exposed CD4+ T cells to activate dendritic cells is reversed by restoration of CD40/CD154 interactions.Blood 107:1989-95.
- Tripathi P, Madan R, Chougnet C, Divanovic S, Ma X, Wahl LM, Gajewski T, Karp CL, Hildeman DA. (2006) An adenoviral vector for probing promoter activity in primary immune cells.J Immunol Methods 311:19-30.
- Montoya CJ, Velilla PA, Chougnet C, Landay AL, Rugeles MT. (2006) Increased IFN-gamma production by NK and CD3+/CD56+ cells in sexually HIV-1-exposed but uninfected individuals.Clin Immunol 120:138-46.
- Hryniewicz A, Boasso A, Edghill-Smith Y, Vaccari M, Fuchs D, Venzon D, Nacsa J, Betts MR, Tsai WP, Heraud JM, Beer B, Blanset D, Chougnet C, Lowy I, Shearer GM, Franchini G. (2006) CTLA-4 blockade decreases TGF-{beta}, indoleamine 2,3- dioxygenase, and viral RNA expression in tissues of SIVmac251-infected macaques.Blood.
- Nilsson J, Boasso A, Velilla PA, Zhang R, Vaccari M, Franchini G, Shearer GM, Andersson J, Chougnet C. (2006) HIV-1 driven regulatory T cell accumulation in lymphoid tissues is associated with disease progression in HIV/AIDS.Blood.
- Chougnet C, Gessani S. (2006) Role of gp120 in dendritic cell dysfunction in HIV infection.J Leukoc Biol.
- Szigligeti P, Neumeier L, Duke E, Chougnet C, Takimoto K, Lee SM, Filipovich AH, Conforti L. (2006) Signalling during hypoxia in human T lymphocytes--critical role of the src protein tyrosine kinase p56Lck in the O2 sensitivity of Kv1.3 channels.J Physiol 573:357-70.
- PA Velilla, MT Rugeles, C Chougnet. Defective antigen-presenting cell function in human
neonates.Clin Exp Immunol, 2006, 121, 251. - C. Chougnet, G.M. Shearer. Regulatory T Cells and HIV/AIDS. Summary of the September 7-8, 2006 Workshop. AIDS Research and Human Retroviruses, 2007, 23, 945.
- A. Boasso, M. Vaccari, A. Hryniewicz, J. Nacsa, D. Fuchs, G. Franchini, G.M. Shearer, C. Chougnet. Increased expression of regulatory T cell markers and indoleamine 2,3 dioxygenase in spleen and gut during active viral replication in SIV-infected macaques.J. Virol. 2007, 81, 11593-11603.
- P.A. Velilla, M.T. Shata, C. Silva Lages, J. Ying, C. Fichtenbaum, C. Chougnet. Effect of low-dose IL-2 immunotherapy on frequency and phenotype of regulatory T cells and NK cells in HIV/HCV co-infected patients.AIDS Research and Human Retroviruses, 2008, 24, 52-61.
- S. Li, E.J. Gowans, C. Chougnet, M. Plebanski, U. Dittmer. Natural regulatory T cells and persistent viral infection.J. Virol. 2008, 82, 21-30 (Review).
- P.A. Velilla, C.J. Montoya, A. Hoyos, M.E. Moreno, C. Chougnet, M.T. Rugeles. Effect of intrauterine HIV-1 exposure on the frequency and phenotype of uninfected newborns’ dendritic cells.Clin Immunol, 2008, 126, 243-50.
Professional Organization Memberships
- American Association of Immunologists
- Society for Pediatric Research
Teaching
- 1987-1991: Post-graduate course, "Prevention of Endemic Diseases", Section: Malaria, Paris VII University, Paris, France.
- 1992: Post-graduate course, "Tropical Microbiology", Section: Immunology, Pasteur Institute, Paris, France.
- 1998: Post-graduate course, "Host-Parasites Interactions", Section: Mucosal Biology, Physiology and Immunity. Paris V University, Paris, France
- 2003-present: Member of the Admission Committee, Immunobiology Graduate Program, University of Cincinnati.
- 2004-present: Teaching in "Immunobiology Foundations I" and "Immunobiology Foundations II", Immunobiology Graduate Program, University of Cincinnati.
Special Interests
antigen-presenting cells, HIV research, ontogeny of immune responses
Honors:
"Medaille d'Or de l'Internat en Pharmacie" (gold metal, Pharmacy residency program), CHU Toulouse, France, 1985
Ph. D. award from Paris V University, Paris, France, 1991
Habilitation à diriger les recherches" (qualification to direct investigations, awarded by the French University), Université Paris V, Paris, France, 1998.
Presentations:
DOMESTIC
Involvement of monocytes in the Pathogenesis of HIV/AIDS. Joint meetings of The Cell Proliferation Society and 6th annual Chesapeake Cytometry Consortium Conference, Greenbelt, MD, USA, March 1999.
Monocyte-derived dendritic cells from HIV+ patients produce normal amounts of Interleukin-12. The Hopkins, NIH, Wistar Joint IL-12 meeting, Baltimore, MD, USA, May 1999.
Involvement of monocytes in the Pathogenesis of HIV/AIDS. Twelfth Annual Meeting of the Association of Medical Laboratory Immunologists, Bethesda , MD, USA, July 1999.
Antigen-presenting cells in the Pathogenesis of HIV/AIDS: Dysregulated cytokine profile and modified phenotype. 2000 Keystone Symposium on Cytokines and disease, Snowbird, UT, USA, April 2000.
In vivo CD40-CD154 (CD40Ligand) interaction induces integrated HIV expression by antigen-presenting cells in an HIV-1-transgenic mouse model. NIH Cytokine interest group, Frederick, MD, September 2000.
Co-organizer of an international workshop, entitled "Antigen-presenting cell defects in HIV/AIDS", sponsored by the Office of AIDS Research and the National Cancer Institute. October 2001, Arlie House, VA, USA.
Chairperson: Antigen-presenting cells/HIV interactions. Workshop on "Antigen-presenting cell defects in HIV/AIDS", sponsored by the Office of AIDS Research and the National Cancer Institute. October 2001, Arlie House, VA, USA.
INTERNATIONAL
Antigen-presenting cells: a common immunologic problem in HIV/AIDS and Cancer? 1999 International Meeting of the Institute of Virology, Baltimore, USA, August 1999.
Maternal influence on fetal/neonatal antigen-presenting cell function. MRC Gambia/WHO symposium on Immunisation of newborns in the tropics, Banjul, The Gambia, November 1999.
Disscussant: Immune response in early life: limitations and prespectives. MRC Gambia/WHO symposium on Immunisation of newborns in the tropics, Banjul, The Gambia, November 1999.
Editing:
Reviewer for: Blood, Clinical and Diagnostic Laboratory Immunology, Immunology Letters, Journal of Infectious Diseases, Journal of Leukocyte Biology, Pediatric Research
Ad hoc reviewer for grant proposals: Swiss National Science Foundation
