University of Cincinnati Department of Pediatrics

Anil Mishra, PhD

Appointment

Assistant Professor

Email

anil.mishra@cchmc.org

Phone

513-636-3347

Fax

513-636-3310

Bio

Anil Mishra, PhD, scientific objective includes elucidating the mechanism for the recruitment of inflammatory cells into the mucosal allergic tissue. Dr. Mishra is actively involved in managing a research program focused on understanding the molecular mechanisms of eosinophilic esophageal and lower gastrointestinal tract inflammation.

Dr. Mishra's important contribution was to establish that eosinophils are the resident cell that home prenatally in the gastrointestinal tract and the gastrointestinal tract (esophagus to colon) constitutively expresses eotaxin (an eosinophil selective chemoattractant). These finding indicated that the esophagus is devoid of resident eosinophils at baseline and eotaxin is not sufficient for eosinophil trafficking and homing into the tissues. In an effort to further understand the mechanisms and significance of eosinophil accumulation in the lung and esophagus at disease states, Dr. Mishra prioritized his research to focus on lung inflammation associated eosinophilic esophagitis, aiming to gain insight into this poorly understood disease. He developed a murine model of asthma associated eosinophilic esophagitis. These findings implicated aeroallergens in the etiology of EE and suggested that esophageal eosinophilic inflammation is mechanistically associated with pulmonary inflammation.

Recently, Dr. Mishra showed that CD4 T cells dependency is less important in the esophageal eosinophilic inflammation compared to the lung and IL-5 induced esophageal eosinophilia promotes tissue remodeling. Dr. Mishra's is an elected fellow of the American Academy of Allergy, Asthma and Immunology and the American Association of Gastroenterology. He has over a 50 articles on molecular mechanisms of the pulmonary toxicity and allergic responses. His research has been supported by the National Institutes of Health to understand the mechanism that induce eosinophilic esophagitis.

Credentials

PhD: Kanpur University, Kanpur, India

Awards and Honors

1987: Student Award for attending research workshop at Duke University Medical
Center, Durham, NC.
1988: Visiting Scientist Award, GSF, Munchen, Germany.
1993: Research Fellowship Award from National Research Council, Washington, DC, on the grant of Ozone effect on the lungs of rodents.
1996: Best Research Work Presentation Award, Tulane University, New Orleans, LA. p53 role in the development of lung fibrosis.
1997: Young Investigator Award from Central Society of Clinical Research,
Washington, DC, on KGF regulates surfactant proteins.
1999: Travel grant from American Academy of Allergy and Asthma
to present eosinophils homing into GI tract.
2000: Travel grant from American Academy of Allergy and Asthma. To present eosinophils traffickingto the esophagus.
2004: Elected fellow in the American Academy of Allergy, Asthma and Immunology, Milwaukee, WI
2008: Elected fellow in American Association of Gastroenterology

Research

Dr. Mishra's scientific objective includes elucidating the mechanism for recruitment of inflammatory cells into mucosal allergic tissue. He is aiming to elucidate the mechanisms involved in the development of hypersensitivity responses in the esophagus and the connection between pulmonary and esophageal inflammation. He developed first murine model of eosinophilic esophagitis. His research interest also includes in understanding the mechanism of aeroallergen-induced allergic responses in the lung and gastrointestinal tract.

Research Grants and Contracts

(1) National Institute of Health (NIH) Anil Mishra, Principal Investigator 2005-2010 RO1 Mechanistic analysis of eosinophilic esophagitis.

(2) National Institute of Health (NIH) Anil Mishra, Principal Investigator (Pending) RO1 Role of Il-15 in the pathogenesis of eosinophilic esophagitis.

(3) National Institute of Health (NIH) Anil Mishra, Principal Investigator (Pending) RO1 Cellular and molecular mechanism in the pathophysiology of eosinophilic esophagitis."

Publications, Most Recent

Mishra A, Hogan SP, Lee, JJ, Foster PS, Rothenberg ME. 1999. Fundamental signals regulating eosinophil homing into the gastrointestinal tract. J Clin Invest 103: 1719-1727.

Mishra A, Doyel N, Martin WJ. Bleomycin –mediated pulmonary toxicity: evidence for a p53-mediated response. Am J Respir Cell Mol Biol 22: 543-549, 2000.

Hogan SP, Mishra A, Brandt E, Foster PS, Rothenberg ME. 2000. A critical role for eotaxin in experimental oral antigen-induced eosinophilic gastroenteritis. Proc Natl Acad Sci USA 97: 6681-6686

Mishra A, Hogan SP, Brandt E, Rothenberg ME. 2000. Peyer’s patch eosinophils: identification, characterization,and regulation by mucosal allergen exposure, interleukin-5, and eotaxin. Blood 96:1538-44.

Mishra A, Beck D, Weaver T, Rothenberg ME. 2001. Allergic airway inflammation inhibits the human surfactant protein C promoter in transgenic mice. J Biol Chem 276: 8453-8459.

Mishra A, Hogan SP, Brandt E, Rothenberg ME. 2001. An etiological role for aeroallergens in experimental esophagitis. J Clin Invest 107: 83-90.

Hogan SP, Mishra A, Brandt EB, Royalty MP, Pope SM, Zimmermann N, Foster PS, Rothenberg ME. 2001. A pathological role for eotaxin and eosinophils in experimental oral antigen induced eosinophilic gastrointestinal inflammation. Nature Immunol 2: 353-360.

Mishra A, Hogan SP, Brandt EB, Wagner, N, Crossman MW, Foster PS, Rothenberg ME. 2002. Enterocyte expression of the eotaxin and interleukin-5 transgenes induces compartmentalized dysregulation of eosinophil trafficking. J Biol Chem 277: 4406-4412

Mishra A, Hogan,SP, Brandt,E and Rothenberg ME. 2002. Interleukin-5 promotes eosinophil trafficking to the esophagus. J Immunol, 168: 2464-2469.

Mishra A, Rothenberg ME. 2003. Intratracheal IL-13 induces eosinophil esophagitis is regulated by IL-5, eotaxin-1 and STAT6. Gastroenterology 125(5): 1419-27.

Mishra A, Schlotman J, Wang M and Rothenberg ME. 2006. Critical role for adaptive T cell immunity in experimental eosinophilic esophagitis in mice.J. Leukocyte Biology. 81, 916-924.

Mishra A, Wang M, Schlotman J, Nikolaidis MN, DeBrosse CW, Karow ML and Rothenberg ME. 2007. Resistin-Like Molecule (RELM)-β is an allergen-Induced cytokine with inflammatory and remodeling activity in the murine lung. AJP Lung Cell Mol Physiology, 293, 305-313.

Mishra A, Wang M, Pemmaraju VR, Collins MU, Putnam PE, et al. 2007. Esophageal remodeling develops as a consequence of tissue specific IL-5 induced eosinophilia.Gastroenterology.134, 204-14.

Presentations, Most Recent

The American Thoracic Society Annual Meeting, "Allergic airway inflammation inhibits the expression of human surfactant protein C promoter in transgenic mice", Toronto, Canada, 2000.

The 2nd International meeting of International Eosinophil Society, "Aeroallergens role in the development of experimental esophagitis in mice", Banff, Canada, 2001.

The American Academy of Allergy, Asthma and Immunology Annual Meeting Expression of eotaxin in enterocytes induces intestinal eosinophilia.2002 at New York.

The 3rd International meeting of International Eosinophil Society Lymphocyte mediated immunity is essential for the development of experimental allergic eosinophillic esophagitis. 2003 at Aspen, CO.

The American Academy of Allergy, Asthma and Immunology Annual Meeting. Resistin-Like Molecule (RELM)-α and β Are Allergen-Induced Cytokines With Potent Remodeling Activity In The Murine Lung. 2004 at San Francisco, CA.

American Academy of Allergy, Asthma and Immunology Annual Meeting. Critical role of antigen specific lymphocytes in the induction of asthma associated eosinophilic esophagitis,. Maim, Florida, March 2005.

First International Conference on Gastrointestinal eosinophils. Molecular mechanism of anti-cytokine treatment of eosinophilic esophagitis. September 18-19, 2006, Orlando, Fl.

The 4th International Meeting of International Eosinophil Society, Role of T regulatory cells in the development of eosinophilic esophagitis. Salt Lake City, CO July 18-21, 2007.

American Gastrointestinal Association Annual Digestive Disease Week, Role of Il-15 in the pathogenesis of eosinophilic esophagitis. San Diego, CA, May 8-12, 2008.