University of Cincinnati Department of Pediatrics

S. Steven Potter, PhD

Title

Professor

Appointment

Professor of Pediatrics

Email

steve.potter@cchmc.org

Phone

513-636-4850

Fax

513-636-4317

Credentials

PhD: University of North Carolina, Chapel Hill, 1976 (Bacteriology and Immunology)

Postdoctoral Fellowship: Harvard Medical School, Boston, Mass. 1976-1978

Awards and Honors

President's Undergraduate Research Grant

Baccalaureate cum laude, with departmental Honors

National Institutes of Health Predoctoral Trainee

National Institutes of Health Postdoctoral Research Fellow

National Institutes of Health Career Development Award, 1982-1987

Research

S. Steven Potter, PhD, conducts research in the area of mammalian developmental genetics. His research focuses on three areas. The first is to better define the genetic regulatory network that regulates formation of the kidney. The second is to study the functions of the murine clustered homeobox (Hox) genes, which are believed to represent master switches that control the developmental destinies of groups of cells. The third area of study cytoplasmic re-programming of nuclei in cloned mice.

A variety of techniques are used, with emphasis on microarrays and targeted manipulation of the mouse genome. Microarrays permit global analysis of gene expression states. They allow us to move from study of a single gene to the characterization of complex gene expression profiles that define distinct differentiation states. And gene targeting allows us to determine developmental functions of genes by generating mutations that can be null or subtle, organism wide or organ specific, and that can be triggered as a function of developmental time. The developmental outcomes define developmental roles.

Current projects include the use of microarrays to create a gene expression atlas of the developing kidney. We are also using microarrays to study the altered global gene expression patterns that occur in cloned embryos. Other projects use microarrays to identify downstream targets of transcription factors that regulate early kidney development. We are also making mice with targeted versions of novel genes with interesting functional motifs that we have identified expressed in the developing kidney.

Research Grants and Contracts

04/01/06 - 03/31/10, National Institutes of Health (R01 DK 061916), Development of Metanephric Mesenchyme. Annual Direct Cost: $205,000, Project Period Direct Cost: $820,000.

09/30/04 - 07/31/09, National Institutes of Health (U01 DK 070251), Global Gene Expression Atlas of the Developing Kidney. Annual Direct Cost: $241,782, Project Period Direct Cost: $1,256,457.

Stipend and benefit support is available to qualified candidates through NHLBI Training Grant: T-32 HL07382-30: "Training In Cardiovascular Biology", Dr. Arnold Schwartz, Principal Investigator.

Publications, Most Recent

S. Steven Potter's publications as listed by PubMed

Lu MF, Cheng HT, Kern MJ, Potter SS, Tran B, Diekwisch TG, Martin JF. prx-1 functions cooperatively with another paired-related homeobox gene, prx-2, tomaintain cell fates within the craniofacial mesenchyme.Development 126:495-504, 1999.

Lu MF, Cheng HT, Lacy AR, Kern MJ, Argao EA, Potter SS, Olson EN, Martin JF Paired-related homeobox genes cooperate in handplate and hindlimb zeugopod morphogenesis.Developmental Biology 205:145-57, 1999.

Li H, Schrick JJ, Fewell GD, MacFarland KL, Witte DP, Bodenmiller DM, Hsieh-Li H-M, Su C-Y, Potter SS. Novel strategy yields candidate Gsh-1 homeobox gene targets using hypothalamus progenitor cell lines.Developmental Biology 211:64-76, 1999.

Supp DM, Brueckner M, Kuehn MR, Witte DP, Lowe LA, McGrath J, Corrales J, Potter SS. Targeted deletion of the ATP binding domain of left-right dynein confirms its role in specifying development of left-right asymmetries.Development 126:5495-5504, 1999.

Brunskill EW, Witte DP, Shreiner AB, Potter SS. Characterization of Npas3, a novel basic-helix-loop helix PAS gene expressed in the developing mouse nervous system.Mechanisms of Development 88(2):237-241, 1999.

Branford WW, Benson GV, Ma L, Maas RL, Potter SS. Characterization of Hoxa-10/Hoxa-11 transheterozygotes reveals functional redundancy and regulatory interactions.Developmental Biology 224:373-387, 2000.

Supp DM, Potter SS, Brueckner M. Molecular motors: the driving force behind mammalian left–right development.Trends in Cell Biology 10(2):41-45, 2000.

Bates MD, Schatzman LC, Lints T, Hamlin PE, Harvey RP, Potter SS. Structural and Functional Characterization of the mouse Hlx Gene.Mammalian Genome 11:836-842, 2000.

Potter SS, Valerius MT, Brunskill EW. Using progenitor cells and gene chips to define genetic pathways.ES protocols. In press 2000.

Toresson H, Potter SS, Campbell K. Genetic control of dorsal-ventral identity in the telencephalon: opposing roles for Pax6 and Gsh2.Development 127:4361-4371, 2000.

Bates DB, Schatzman LC, Harvey RP, Potter SS. Two CCAAT boxes in a novel inverted repeat motif are required for Hlx Homeobox gene expression. BBA 1519:96-105, 2001.

Yun K, Potter S, Rubenstein JLR. Gsh2 and Pax6 play complementary roles in dorsoventral patterning of the mammalian telencephalon.Development 128:193-205, 2001.

Brunskill EW, Witte DP, Yutzey KE, Potter SS. Isolation of early cardiomyocyte cell lines from Nkx2.5/simian virus 40 large tumor antigen transgenic mice. Dev Biol 235:507-520, 2001.

Larsen WJ, Sherman L, Potter SS, Scott WJ. Human embryology.A textbook originally written by W. Larsen, with the third edition update written by Sherman, Potter, and Scott after Dr. Larsen's untimely death. 2001.

Patterson LT, Pembauer M, Potter SS. Hoxa 11 and Hoxd 11 regulate branching morphogenesis of the ureteric bud in the developing kidney.Development 128:2153-2161, 2001.

Zhao Y, Potter SS. Functional specificity of the Hoxa 13 homeobox. Development 128:3197-3207, 2001.

Zhao Y, Potter SS. Functional comparison of the Hoxa 4, Hoxa10 and Hoxa 11 homeoboxes.Dev Biol 244:21-36, 2002.

Potter SS, Valerius MT, Brunskill EW. Using progenitor cells and gene chips to define genetic pathways. ES protocols. Edited by K. Turksen. Methods in Molecular Biology 185:269-284, 2002.

Valerius MT, Patterson LT, Potter SS. Developmental function and gene expression profiles of metanephric mesenchyme cell lines derived from Hoxa 11-SV40 Tag transgenic mice.Mechanisms of Dev 110:151-164, 2002.

Bodenmiller DP, Baxter CS, Hansen DV, Potter SS. Phylogenetic analysis of Hoxa 11 sequences reveals absence of transposable elements, conservation of transcription factor binding sites, and suggests antisense coding function.DNA Seq 13:77-83, 2002.

Valerius MT, Feng Y, Potter SS. Hoxa 11 is upstream of Integrin alpha-8 expression in the developing kidney. PNAS USA 99:8090-8095, 2002.