Pulmonary Biology

Anne-Karina T. Perl, MS, PhD

Appointment

Assistant Professor of Pulmonary Biology, University of Cincinnati College of Medicine

Email

anne.perl@cchmc.org

Phone

513-636-6084

Fax

513-636-7868

Credentials

PhD: University of Vienna, Austria, 1998

Position History

March 1998 to October 1998 Postdoctoral Fellow in the Lab of Gerhard Christofori, PhD, Institute of Molecular Pathology (IMP), Vienna, Austria

January 1999 to August 2002 Postdoctoral Fellow in the lab of Jeff Whitsett, Department of Pediatrics, Children's Hospital Medical Center, Cincinnati, OH, USA

September 2002to Present Assistant Professor of Pulmonary Biology, Department of Pediatrics, Children's Hospital Medical Center, Cincinnati, OH, USA

Awards and Honors

May 2000 to May 2001 Second year extension of FWF Erwin Schrödinger Fellowship J1959-GEN for work on FGF and FGFR signaling during lung morphogenesis

May 1999 to May 2000 Stipend with a FWF Erwin Schrödinger Fellowship J1746-GEN for work on FGF and FGFR signaling during lung morphogenesis

September 1998 Young Scientist of the year 1998 of the Austrian Society of Genetics and Gentechnology

Research

Visit Dr. Perl's lab site.

Research Grants and Contracts

5P50 HL56387-07 (Whitsett)
09/30/01 - 07/31/06; 50%
NIH/NHLBI
Specialized Center of Research (SCOR)

Transcriptional Control of Respiratory Epithelial Cell Differentiation (TTF-1, HNF-3B)

The major goals of this project are 1a) Pulmonary cell lineage analysis using dox-regulated, cre-lox cell marking; 1b) identification of respiratory epithelial stem cells and lineage, with ALV-A; 2) role of TTF-1 in lung morphogenesis and respiratory epithelial cell differentiation; 3) role of HNF-3B on lung morphogenesis and respiratory epithelial cell differentiation; and 4) role of TTF-1 and HNF-3B in pathogenesis of congenital malformations and repair of the developing human lung.

2 P50 HL56387-07 (Whitsett/ Shannon)
9/30/01 - 7/31/06; 25%
NIH/NHLBI
Specialized Center of Research (SCOR)

Fibroblast Growth Factors in Lung Morphogenesis

The major goals of this project are: 1) To define the role of FGF-10 in lung epithelial cell growth and differentiation; 2) To determine the FGF's that are involved in specification of the distal lung epithelial phenotype; 3) To determine the effects of FGF's on lung morphogenesis and differentiation during late gestation and alveolarization; 4) To determine the role of FGF's in the response of the neonatal lung to oxidant injury.

Publications, Most Recent

Perl, AKT, Wilgenbus, P, Dahl, U, Semb, H, and Christofori, G (1998). A causal role for E-Cadherin in the transition from adenoma to carcinoma. Nature 392, 190-193.

Perl, AKT, Dahl, U, Wilgenbus, P, Cremer, H, Semb, H, and Christofori, G (1999)
Neural Cell Adhesion Molecule (N-CAM) modulates the metastatic dissemination of pancreatic tumor cells.Nature medicine, Vol. 5, 3 286-291.

Esni, F, Täljedal, I-B, Perl, AKT, Cremer H, Christofori, G, and Semb, H (1999)
N-CAM is required for cell type segregation and normal ultrastructure in pancreatic islets. J Cell Biol. 1999 Jan; 144(2): 325-37.

Perl AKT, Whitsett JA (1999). Molecular mechanisms controlling lung morphogenesis. Review, Clin. Genet 1999 Jul; 56(1) 14-27.

Clark JC, Tichelaar JW, Wert SE, Itoh N, Perl AKT, Stahlman MT, Whitsett JA (2001).
FGF10 disrupts lung morphogenesis and causes pulmonary adenomas in vivo.Am J Physiol Lung Cell Mol Physiol. 280 (4): L705-15.

Whitsett JA, Glasser SW, Tichelaar JW, Perl AKT, Clark JC, Wert SE (2001). Transgenic models for study of lung morphogenesis and repair:
Parker B. Francis lecture.Chest., 120(1 Suppl): 27S-30S.

Perl AKT; Tichelaar JW; Whitsett JA (2002)
Conditional gene expression in the respiratory epithelium of the mouse.
Transgenic Res. 2002 Feb; 11(1): 21-9.

Whitsett JA, Clark JC, Picard L, Tichelaar JW, Wert SE, Itoh N, Perl AKT, Stahlman MT (2002). Fibroblast growth factor 18 influences proximal programming during lung morphogenesis.J Biol Chem. 2002 Jun 21; 277(25): 22743-9.

Perl, AKT, Wert SE, Nagy A, Lobe CG, Whitsett JA (2002). Early restriction of peripheral and proximal cell lineages during formation of the lung.PNAS 99 (16) 10482-10487.

Hokuto I, Perl AKT, Whitsett JA (2003) (co-first author). Prenatal, but not postnatal inhibition of FGF-receptor signaling causes emphysema.J Biol Chem 278:415-21.

Perl AKT, Hokuto I, Impagnatiello MA, Christofori G, Whitsett JA (2003). Temporal effects of Sprouty on lung morphogenesis.Dev Biol. 2003 Jun 1;258(1):154-68.

Hokuto I, Perl AK, Whitsett JA. FGF-signaling is required for pulmonary homeostasis following hyperoxia.Am J Physiol Lung Cell Mol Physiol. 2004 Mar;286(3):L580-7. Epub 2003 Nov 14.

Hokuto I, Ikegami M, Yoshida M, Takeda K, Akira S, Perl AK, Hull WM, Wert SE, Whitsett JA. Stat-3 is required for pulmonary homeostasis during hyperoxia.J Clin Invest. 2004 Jan;113(1):28-37.

Miller LA, Wert SE, Clark JC, Xu Y, Perl AK, Whitsett JA. Role of Sonic hedgehog in patterning of tracheal-bronchial cartilage and the peripheral lung.Dev Dyn. 2004 Sep;231(1):57-71.

Perl AK, Ralf Kist R, Shan ZY, Scherer G., Whitsett JA. Normal lung development and function after Sox9 inactivation in the respiratory epithelium.Genesis 2005 Jan;41(1):23-32.

Perl AK, Wert SE, Loudy DE, Shan Z, Blair PA, Whitsett JA. Conditional Recombination Reveals Distinct Subsets of Epithelial Cells in Trachea, Bronchi, Alveoli.Am J Respir Cell Mol Biol. 2005 Jul 29.