Pulmonary Biology

Machiko Ikegami, MD, PhD

Appointment

Professor of Pediatrics

Email

machiko.ikegami@cchmc.org

Phone

513-636-8661

Fax

513-636-8691

Bio

Dr. Ikegami studies lung and surfactant metabolism in vivo using transgenic mice. Her research includes the effects of ventilation style on surfactant metabolism in immature lungs and the functions of surfactant components.

Credentials

MD: Tokyo Women's Medical University, Tokyo, Japan, 1971

PhD: Tokyo Women's Medical University, Tokyo, Japan, 1980

Research Grants and Contracts

NIH NHLBI SCOR Pathology of Lung Development. Physiology Core, Principal Investigator. (Jeffery Whitsett, Program Director); September, 1996 to August 2001.
NIH NHLBI PPG Surfactant Homeostasis in Health and Disease. Project 1: Alveolar Homeostasis of Surfactant Lipid and proteins, Co-Investigator; Metabolic and Function Core, Principal Investigator (Alan Jobe, Program Director); July, 1999 to June, 2004.
NIH-NHLBI RO1 Research Grant Role of Surfactant Protein D in Surfactant Homeostasis, Principal Investigator; September, 2000 to August, 2004.
NIH-HNLBI RO1 Research Grant New Mediators of Lung Maturation, Co-Investigator; September, 2000 to August, 2004.
NIH-NHLBI Antecedents to Lung Injury in the Preterm, Co-Investigator; February, 2001 to January, 2006.
NIH-NHLBI Structure/Function Analyses of SP-B in Transgenic Mice, Co-Investigators; May, 2001 to April, 2006.

Publications, Most Recent

Jobe, A.H., Newnham, J.P., Moss, T.J., and Ikegami, M. Differential effects of maternal betamethasone and cortisol effects on lung maturation and growth in fetal sheep.Am. J. Obstet. Gynecol. 188:22-28, 2003.

Epaud, R., Ikegami, M., Whitsett, J.A., Jobe, A.H., Weaver, T.E., and Akinbi, H.T. Surfactant protein (SP-B) inhibits endotoxin-induced lung inflammation. Am. J. Repir. Cell & Molecular Biol. 28:373-378, 2003.

Kallapur, S.G., Jobe, A.H., Ikegami, M., and Bachurski, C.J. Increased IP-10 and MIG expression after intra-amniotic endotoxin in preterm lamb lung.Am. J. Respir. Crit. Care Med. 167:779-786, 2003.

Collins, R.A., Ikegami, M., Korfhagen, T.R., Whitsett, J.A., and Sly, P.D. Changes in lung function with age in mice deficient in surfactant protein D give clues to the relative contributions of lung structure and surface-acting forces to parenchymal mechanics in vivo.Pediatr. Res. 53:463-467, 2003.

Ikegami, M., Dhami, R., and Schuchman, E.H. Altered surfactant metabolism, composition and function in acid sphingomyelinase deficient mouse model of Niemann-Pick Disease.Am. J. Physiol. 284:L518-L525, 2003.

Glasser, S.W., Detmer, E.A., Ikegami, M., Ross, G.F., Wert, S.E., Na, C-L., Stahlman, M.T. and Whitsett, J.A. Interstitial pneumonitis in SP-C gene targeted mice.J. Biol. Chem. 278:14291-14298, 2003.

Kallapur, S.G., Kramer, B.W., Moss, T.J., Newnham, J.P., Jobe, A.H., Ikegami, M., and Bachurski, C.J. Maternal glucocorticoids increase endotoxin induced lung inflammation in preterm lambs. Am. J. Physiol. 284:L633-L642, 2003.

Ikegami, M., Moss, T.J.M., Kallapur, S.G., Mulrooney, N., Kramer, B.W., Nitsos, I., Bachurski, C.J., Newnham, J. P., and Jobe, A.H. Minimal lung and systemic responses to TNF in preterm sheep. Am. J. Physiol. 285:L121-L129, 2003.

Kramer, B.W., Jobe, A.H., and Ikegami, M. Monocyte function in preterm, term and adult sheep. Pediatr. Res. 54:52-57, 2003.

Melton, K.R., Nesslein, L.L., Ikegami, M., Tichelaar, J.W., Clark, J.C., Whitsett, J.A., and Weaver, T.E. SP-B deficiency causes respiratory failure in adult mice. Am. J. Physiol. 285:L543-L549, 2003.

Liu, C., Ikegami, M., Stahlman, M.T., Dey, C.R., and Whitsett, J.A. Inhibition of alveolarization and altered pulmonary mechanics in mice expressing GATA-6. Am. J. Physiol. 285:L1246-L1254, 2003.

Hokuto, I., Ikegami, M., Yoshida, M., Takeda, K., Akira, S., Perl, A.T., Hull, W.M., Wert, S.E. and Whitsett, J.A. Stat-3 is required for pulmonary homeostasis during hyperoxia. J. Clin. Invest. 113: 28-37, 2004.

Ikegami, M., Kallapur, S.G. and Jobe, A.H. Responses of ventilated lambs to intra-amniotic endotoxin given 4d before preterm delivery. Am. J. Physiol. 286:L573-L579, 2004.

Wan, H., Kaestner, K.H., Ang, S-L., Ikegami, M., Finkelman, F.D., Stahlman, M.T., Fulkerson, P., Rothenberg, M.E., and Whitsett, J.A. Foxa2 regulates alveolarization and goblet cell hyperplasia. Development, 131:953-964, 2004.

Mulrooney, N., Jobe, A.H., and Ikegami, M. Lung inflammatory responses to intratracheal IL-1 in ventilated preterm lambs.Pediatr. Res. 55:682-687, 2004.

Li, J., Ikegami, M., Na, C-L, Hamvas, A., Espinassous, Q, Chaby, R., Nogee, L.M., Weaver, T.E., and Johansson, J. N-terminally extended surfactant protein (SP)C isolated from SP-B deficient children has reduced surface activity and inhibited lipopolysaccharide binding. Biochemistry 43:3891-3898, 2004.

Le Cras, T.D., Hardie, W.D., Deutsch, G.H., Albertine, K.H., Ikegami, M., Whitsett, J.A., and Korfhagen, T.R. Transient induction of TGF disrupts lung morphogenesis causing pulmonary disease in adulthood. Am. J. Physiol., 287:L718-L729, 2004.

Pillow, J.J., Jobe, A.H., Collins, R.A., Hantos, Z., Ikegami, M., Moss, T.J., Newnham, J.P., Willet, K.E., and Sly, P.D. Variability in preterm lamb lung mechanics after intra-amniotic endotoxin is associated with changes in surfactant pool size and morphometry. Am. J. Physiol. 287:L992-L998, 2004.

Wan, H., Xu, Y., Ikegami, M., Stahlman, M.T., Kaestner, K.H., Ang, S-L, and Whitsett, J.A. Foxa2 is required for transition to air breathing at birth. Proc. Natl. Acad. Sci., USA. 101:14449-14454, 2004.

Kallapur, S.G., Bachurski, C., Le Cras, T.D., Joshi, S.N., Ikegami, M., and Jobe, A.H. Vascular changes following intra-amniotic endotoxin in the preterm lamb lung. Am. J. Physiol., 287:L1178-L1185, 2004.

Kramer, B.W., Ikegami, M., Moss, T.J.M., Nitsos, I., Newnham, J.P., and Jobe, A.H. Endotoxin induced chorioamnionitis modulates innate immunity of monocytes in preterm sheep.Am. J. Respir. Crit. Care Med., 171:73-77, 2005.

Ryan, M.A., Qi, X., Ikegami, M., Perez-Gil, J., Johansson, J., and Weaver, T.E. Mapping and analysis of the lytic and fusogenic domains of surfactant protein B (SP-B). Biochemistry, 44:861-872, 2005.

Mulrooney, N., Champion, Z., Moss, T.J.M., Nitsos, Il, Ikegami, M., and Jobe, A.H. Surfactant and physiological responses of preterm lambs to continuous positive airway pressure. Am. J. Respir. Crit. Care Med., 171:488-493, 2005.

Ikegami, M., Na, C-L., Korfhagen, T., and Whitsett, J.A. Surfactant protein D influences surfactant ultrastructure and uptake by alveolar type II cells. Am. J. Physiol., 288:L552-L561, 2005.

Kallapur, S.G., Nitsos, I., Moss, T.J.M., Kramer, B.W., Newnham, J.P., Ikegami, M., and Jobe, A.H. Chronic endotoxin exposure does not cause sustained structural abnormalities in the fetal sheep lungs. Am. J. Physiol., In press.

Ikegami, M., Le Cras, T.D., Hardie, W.D., Stahlman, M.T., Whitsett, J.A. and Korfhagen, T.R. TGF perturbs surfactrant homeostasis in vivo. Am. J. Physiol. In press.