Cincinnati Center for Eosinophilic Disorders Research
Eosinophilic Gastrointestinal Disorder Diagnostics

Eosinophilic Gastrointestinal Disorder Diagnostics

Our research integrates multidisciplinary basic studies, with particular emphasis on modern genetic approaches, translational research employing novel in vivo and ex vivo systems developed by our laboratory and clinical studies designed to test proof-of-concept theories and novel therapeutics directly in humans.

We strive to translate our research into clinical utility and practice, as exemplified by our development of diagnostics for eosinophilic gastrointestinal disorders.

Our Tests

Current Tests

Test	Description	Reference	Contact
Esophageal Peak Eosinophil Count	≥15 eosinophils / high-power microscopic field and clinical symptoms	Furuta et al. Gastroenterology. 2007 Liacouras et al. J Allergy Clin Immunol. 2011	Margaret H. Collins, MD Professor of Pathology & Laboratory Medicine Margaret.collins@cchmc.org
Eosinophilic Esophagitis Diagnostic Panel (EDP; clinically available as EoGenius(TM) from Miraca Life Sciences)	Next-generation molecular diagnostic with high accuracy for eosinophilic esophagitis	Wen et al. Gastroenterology. 2013 Butz et al. Gastroenterology. 2014	Marc E. Rothenberg, MD, PhD Professor of Pediatrics Rothenberg@cchmc.org
Pediatric Eosinophilic Esophagitis Symptom Severity Module, version 2.0 (PEESS v2.0)	Symptom severity scoring metric for patients with eosinophilic esophagitis	Franciosi et al. BMC Gastroenterology. 2011 Martin et al. J Allergy Clin Immunol. 2015	Lisa J. Martin, PhD Professor of Human Genetics lisa.martin@cchmc.org

Tests in Development

Test	Description	Reference	Contact
Proton pump inhibitor (PPI)-Responsive Esophageal Eosinophilia (PPI-REE) Prediction Panel	Next-generation molecular diagnostic panel (e.g. KCNJ2)	Wen et al. J Allergy Clin Immunol. 2015	Marc E. Rothenberg, MD, PhD Professor of Pediatrics Rothenberg@cchmc.org
Esophageal microRNA Panel	MicroRNA panel of esophageal biopsy	Lu et al. J Allergy Clin Immunol. 2012	Marc E. Rothenberg, MD, PhD Professor of Pediatrics Rothenberg@cchmc.org
Histology Scoring System (HSS)	An extended analysis of histologic pathology including score and grade	Collins et al. J Allergy Clin Immunol. 2012 Collins et al. Dis Esophagus. 2016	Margaret H. Collins, MD Professor of Pathology & Laboratory Medicine Margaret.Collins@cchmc.org
Serum Cytokines	Panel of serum proteins	Konikoff et al. Clin Gastroenterol Hepatol. 2006 Blanchard et al. J Allergy Clin Immunol. 2011	Marc E. Rothenberg, MD, PhD Professor of Pediatrics Rothenberg@cchmc.org
Circulating microRNAs	Panel of serum microRNAs	Lu et al. J Allergy Clin Immunol. 2012	Marc E. Rothenberg, MD, PhD Professor of Pediatrics Rothenberg@cchmc.org
Rapid Biopsy Diagnostic	Same-day polychromatic FACS	Wen et al. J Allergy Clin Immunol. 2017	Marc E. Rothenberg, MD, PhD Professor of Pediatrics Rothenberg@cchmc.org
Eosinophilic Gastritis Panel	Next-generation molecular diagnostic	Caldwell et al. J Allergy Clin Immunol. 2014	Marc E. Rothenberg, MD, PhD Professor of Pediatrics Rothenberg@cchmc.org
Genetic Propensity Panel	Single-nucleotide variant analysis and/or whole-exome sequencing	Rothenberg et al. Nature Genetics. 2010 Kottyan et al. Nature Genetics. 2014	Marc E. Rothenberg, MD, PhD Professor of Pediatrics Rothenberg@cchmc.org
Eosinophil Progenitor (EoP) Cell Level	Measure levels of EoP in the blood	Morris et al. J Allergy Clin Immunol. 2016	Patricia C. Fulkerson, MD, PhD Assistant Professor of Pediatrics

EoE Diagnostic Panel

The Rothenberg Lab developed a molecular diagnostic test called the eosinophilic esophagitis diagnostic panel (EDP). This test uses low-density Taqman quantitative PCR array of biopsy tissue to distinguish patients with eosinophilic esophagitis, the disease activity, and exposure to swallowed glucocorticoids. This test is now clinically available as EoGenius™. Read More

PPI-Responsive Esophageal Eosinophilia

Esophageal eosinophilia can be resistant or responsive to proton pump inhibitors (PPI), representing eosinophilic esophagitis (EoE) if it is resistant and PPI-responsive esophageal eosinophilia (PPI-REE) if it is responsive. A recent, collaborative study by the Wen Lab and Rothenberg Lab shows significant molecular overlap between EoE and PPI-REE transcriptomes, suggesting that these conditions have similar underlying mechanisms. Read More

Cincinnati Center for Eosinophilic Disorders ResearchEosinophilic Gastrointestinal Disorder Diagnostics

Eosinophilic Gastrointestinal Disorder Diagnostics

Our Tests

Test

Description

Reference

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