Varisco Lab

  • Varisco Lab Research

    The principal function of the lung is to facilitate the absorption of oxygen and elimination of carbon dioxide necessary for lung development. This exchange occurs principally at the level of the alveolus. Premature birth often impairs alveolar formation and lung injury can reduce the number of alveoli. Our laboratory is focused on understanding the mechanism which governs alveolar growth and regeneration and how the pulmonary vasculature grows and remodels to maximize ventilation/perfusion matching.

    Our laboratory is specifically focused on how lung matrix remodeling influences alveolar growth and pulmonary vascular morphogenesis. Our preliminary data suggested a role for a novel serine protease, Chymotrypsin-like elastase-1 (CELA1). Murine lung CELA1 was dynamically expressed during both lung development and compensatory lung regrowth following pneumonectomy. CELA1 expression was reduced in hyperoxia, an accepted model of impaired alveolar development. Elastase inhibition and silencing of CELA1 impairs angiogenesis in vitro. CELA1 was expressed in areas of elastin remodeling by in situ zymography. A transgenic mouse with LacZ reporter and the conditional potential is currently in development.

    Our laboratory is also interested in how the pulmonary arterial matrix remodels in response to increased blood flow. We have demonstrated changes in right pulmonary arterial elastin structure following left pneumonectomy and are currently analyzing Next Generation mRNA sequencing data to determine mechanism and developing a model for measuring right pulmonary arterial compliance following pneumonectomy.

  • Publications

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    2013

    Cornett B, Snowball J, Varisco BM, Lang R, Whitsett J, Sinner D. Wntless is required for peripheral lung differentiation and pulmonary vascular development.Dev Biol. 2013 Mar 21. doi:pii: S0012-1606(13)00139-5.

    2012

    Varisco BM, Ambalavanan N, Whitsett JA, Hagood JS. Thy-1 Signals through PPARγ to Promote Lipofibroblast Differentiation in the Developing Lung. Am J Respir Cell Mol Biol. Jun;46(6):765-72. Epub 2012 Jan 20. 2012.

    2011

    Varisco BM. The pharmacology of acute lung injury in sepsis.Adv Pharmacol Sci. 2011:254619. 2011.

    Wong HR, Cvijanovich NZ, Allen GL, Thomas NJ, Freishtat RJ, Anas N, Meyer K, Checchia PA, Lin R, Shanley TP, Bigham MT, Wheeler DS, Doughty LA, Tegtmeyer K, Poynter SE, Kaplan JM, Chima RS, Stalets E, Basu RK, Varisco BM, Barr FE. Validation of a gene expression-based subclassification strategy for pediatric septic shock.Crit Care Med. Nov;39(11):2511-7. 2011.

    Sanders YY, Tollefsbol TO, Varisco BM, Hagood JS. Epigenetic regulation of thy-1 by histone deacetylase inhibitor in rat lung fibroblasts.Am J Respir Cell Mol Biol. Jul;45(1):16-23. 2011.

    2010

    2009

    Varisco BM, Benner KW, Prabhakaran P. Neonatal peritoneal candidiasis successfully treated with anidulafungin add-on therapy.Ann Pharmacother. Nov;43(11):1907-10. 2009.

 
  • Contact Us

    varisco-callout1-200-headshot

    Brian M Varisco MD
    Division of Critical Care Medicine
    Cincinnati Children's Hospital Medical Center
    3333 Burnet Avenue
    Cincinnati, OH 45229-3039

    (513) 803-2485 Office
    (513) 803-3311 Fax
    brian.varisco@cchmc.org