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Research Assistant, IVPaul attended the Eastern Kentucky University and has extensive experience with rodent models of inflammation, sepsis, hemorrhagic shock and myocardial infarction, including the monitoring of cardiovascular parameters in vivo. Paul also performs biochemical and immunohistochemical assays of protein expression in tissues.
paul.hake@cchmc.org
Research Assistant, IV
Patrick is a graduate in Biochemistry of the University of Cincinnati. Patrick plays a major role in the analysis of inflammatory mediators and disease biomarkers by multiplex array technology for our basic science and clinical research studies. Patrick also serves as the general laboratory supervisor managing the daily activities of supply ordering, equipment maintenance and biosafety regulations.
patrick.lahni@cchmc.org
Research Assistant, III
John is a graduate in Chemistry of Northern Kentucky University. John has extensive expertise in the analysis of gene and protein expression, and the development and maintenance of primary cultures of immune cells. John also performs rodent models of hemorrhagic shock and neonatal infection, and assists in maintaining and genotyping mouse colonies.
john.ledford@cchmc.org
Giovanna received her Ph.D. in “Cell Proliferation and Differentiation” from the University of Messina Italy in 2005. Giovanna’s main role is developing and performing in vitro models of inflammation and infection in cell cultures. Her expertise includes also molecular biological techniques of protein expression, cell transfection and RNA silencing.
giovanna.piraino@cchmc.org
Pediatric Critical Care Medicine Fellow
Dr. Samraj enrolled in the Pediatric Critical Care Medicine fellowship in 2010 after completing his residency in Pediatrics from Driscoll Children's Hospital at the Texas A&M University. His research focuses on the pathophysiological mechanisms of myocardial depression that occur during sepsis. He is identifying the molecular mechanisms of mitochondrial dysfunction and testing novel therapeutic approaches to restore energy metabolism in cardiac cells.
ravi.samraj@cchmc.org
Research Assistant, IVmichael.oconnor@cchmc.org
Mike is a graduate in Chemistry of the University of Cincinnati. He plays a major role in molecular biochemical analysis of mitochondrial function and nuclear transcription. In addition, Mike has extensive expertise in primary cell biology, molecular biological cloning and functional nuclear pathway techniques.
Zingarelli Lab
click image for caption.
L to R: Paul Hake, Patrick Lahni, Basilla Zingarelli, Ravi Samraj, Giovanna Piraino, John Ledford
