• Meet the lab

    Headshot photo of Paul William Hake.
    Headshot photo of Paul William Hake.

    Paul William Hake

    Research Assistant, IV
    Paul attended the Eastern Kentucky University and has extensive experience with rodent models of inflammation, sepsis, hemorrhagic shock and myocardial infarction, including the monitoring of cardiovascular parameters in vivo. Paul also performs biochemical and immunohistochemical assays of protein expression in tissues.

    paul.hake@cchmc.org

    Paul William Hake

    Headshot photo of Patrick Lahni.
    Headshot photo of Patrick Lahni.

    Patrick Lahni, BS

    Research Assistant, IV

    Patrick is a graduate in Biochemistry of the University of Cincinnati. Patrick plays a major role in the analysis of inflammatory mediators and disease biomarkers by multiplex array technology for our basic science and clinical research studies. Patrick also serves as the general laboratory supervisor managing the daily activities of supply ordering, equipment maintenance and biosafety regulations.

    patrick.lahni@cchmc.org

    Patrick Lahni

    Headshot photo of John Ledford.
    Headshot photo of John Ledford.

    John Ledford, BS

    Research Assistant, III

    John is a graduate in Chemistry of Northern Kentucky University. John has extensive expertise in the analysis of gene and protein expression, and the development and maintenance of primary cultures of immune cells. John also performs rodent models of hemorrhagic shock and neonatal infection, and assists in maintaining and genotyping mouse colonies.

    john.ledford@cchmc.org

    John Ledford

    Headshot photo of Giovanna Piraino.
    Headshot photo of Giovanna Piraino.

    Giovanna Piraino, PhD

    Research Assistant, III

    Giovanna received her Ph.D. in “Cell Proliferation and Differentiation” from the University of Messina Italy in 2005. Giovanna’s main role is developing and performing in vitro models of inflammation and infection in cell cultures. Her expertise includes also molecular biological techniques of protein expression, cell transfection and RNA silencing.

    giovanna.piraino@cchmc.org

    Giovanna Piraino

    Headshot of Ravi Samraj.
    Headshot of Ravi Samraj.

    Ravi Samraj, MD

    Pediatric Critical Care Medicine Fellow

    Dr. Samraj enrolled in the Pediatric Critical Care Medicine fellowship in 2010 after completing his residency in Pediatrics from Driscoll Children's Hospital at the Texas A&M University. His research focuses on the pathophysiological mechanisms of myocardial depression that occur during sepsis. He is identifying the molecular mechanisms of mitochondrial dysfunction and testing novel therapeutic approaches to restore energy metabolism in cardiac cells.

    ravi.samraj@cchmc.org

    Ravi Samraj

  • Other Lab Members

    Michael Patrick O’Connor, BS

    Research Assistant, IV
    michael.oconnor@cchmc.org

    Mike is a graduate in Chemistry of the University of Cincinnati. He plays a major role in molecular biochemical analysis of mitochondrial function and nuclear transcription. In addition, Mike has extensive expertise in primary cell biology, molecular biological cloning and functional nuclear pathway techniques.

  • Photo of the Zingarelli lab.

    Zingarelli Lab

    click image for caption.