Clinical Translational Research Center

  • Biochemistry Core Laboratory

    At Cincinnati Children’s Hospital Medical Center, the Clinical Translational Research Center’s Biochemistry Core Laboratory provides investigators the following services: 

    • Bone Research Assays
    • Nutritional Status Assays
    • Endocrine Assays
    • Cytokine Assays
    • General Clinical Chemistry Assays
    • Other − Comprehensive Test Menu – see below

    As stipulated by the Scientific Advisory Committee, the Core Laboratory functions to more efficiently serve the common needs of the investigators. The Laboratory has the expertise and facilities to execute most commercially available assays,

    The Core Laboratory can develop new tests with the approval of the CTRC Scientific Advisory Committee. Preference will be given to analyses used by more than one investigator and likely to be used by multiple investigators in the future.

    New assays are recommended by the program director and/or Core Laboratory director. Final approval to include tests in the Core Laboratory portfolio of assays is discussed by the Scientific Advisory Committee prior to providing the tests for investigators.

    The Biochemistry Core Laboratory is CAP (College of American Pathologists) and CLIA (Clinical Laboratory Improvement Amendments) certified.

    The CTRC also offers a Sample Processing Lab.

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    + Bone Research Assays

    The Clinical Translational Research Center Biochemistry Core Laboratory at Children’s Hospital Medical Center provides clinical and research assays for the study bone and mineral metabolism disorders:

    Calcitropic hormones and Vitamin D 

    Parathyroid hormone and vitamin D 25-OH are measured using chemiluminescence immunoassay (CLIA) methodology. 1, 25 dihydroxy vitamin D is measured by a competitive equilibrium radioimmunoassay (RIA).

    Minerals

    Total calcium and phosphorus are measured on an automated analyzer (cobas c 311) using second generation chemistry methods.

    Biochemical Markers of Bone Turnover

    The assorted formation and resorption markers (BALP,PICP, CICP, ICTP, NTx, PYD) are measured using ELISA methodologies.

    Nutritional Status Assays

    The laboratory provides clinical and research assays for assessment of nutritional status:
    Carnitine (total, free, and short chain) is measured using a radio-enzymatic assay. Prealbumin levels are measured using an automated chemistry analyzer. Vitamins A and E are measured using HPLC methodology.

    Endocrine Assays

    The laboratory provides many research assays for the assessment of endocrine health.
    The various hormone levels (ACTH, Cortisol, DHEAS, estradiol, FSH, LH, progesterone 17-OH, testosterone and TSH) can be measured by CLIA, ELISA, or RIA. Obesity related assays such as adiponectin, ghrelin, leptin, and PYY are measured by ELISA.

    Cytokine Assays

    The laboratory currently uses ELISA methodologies for all human cytokine analyses.

    General Clinical Chemistry Assays

    The laboratory provides research assays for many general chemistries using a cobas c 311 automated analyzer. Assays available include lipid profiles, renal function, total bile acids, glucose, C-reactive protein as well as others listed in the Comprehensive Test Menu. Tests not listed but commercially available for the analyzers can be discussed with the laboratory supervisor.

    + Comprehensive Test Menu

    The following assays are available to investigators through the Clinical Translational Research Center Biochemistry Core Laboratory at Cincinnati Children’s Hospital Medical Center. Details of the assay methods and measuring ranges can be obtained by contacting  the laboratory supervisor.

      • ACTH – plasma
      • Adiponectin and HMW Adiponectin – serum
      • Albumin
      • Alkaline phosphatase (serum ALP) – serum
      • Alkaline phosphatase – Bone – (BALP) – serum
      • Alpha-amylase – saliva
      • Bile acids (total)
      • Calcitonin
      • C-reactive protein (CRP) & HS CRP
      • C-terminal propeptide of Type 1 collagen Type I (C1CP)
      • Calcium (CA)
      • Carboxy-terminal-telopeptide of type I collagen-cross-link (ICTP) – serum
      • Carnitine – serum
      • Cholesterol
      • Cortisol – saliva / serum / urine
      • Cotinine – saliva
      • Creatinine – serum / urine
      • DHEAS
      • Estradiol
      • FGF-23 in plasma (pending)
      • FSH
      • Fructosamine
      • Glucose
      • Ghrelin – serum
      • HDL cholesterol
      • Insulin
      • IL-1 beta
      • IL-6 – serum
      • LDL cholesterol
      • Leptin – serum
      • LH
      • Magnesium
      • N-telopeptides of type 1 collagen-cross-linked (NTx) – serum / urine
      • Osteocalcin
      • Phosphorous (P)
      • PIVKA-II
      • Prealbumin – serum
      • Procollagen I C-terminal propeptide (PICP)
      • Progesterone 17-OH
      • PTH N-Tact
      • Pyridinoline cross-links (PYD) – urine
      • PYY – serum
      • Renin – plasma
      • Testosterone
      • Thyroglobulin antibody (TGAB)
      • Thyroid peroxidase antibody (TPOAB)
      • Tissue transglutaminase – IgA (tTg-IgA) - serum
      • TNF-alpha – serum
      • Tartrate resistant acid phosphatase 5b
      • Triglycerides
      • TSH third generation
      • Vitamin A
      • Vitamin D (25-OH)
      • Vitamin D (1,25 dihydroxy)
      • Vitamin E

    + How to Reach Us

    For more information about the Clinical Translational Research Center Biochemistry Core Laboratory, contact Theresa Kenney, laboratory supervisor, at 513-636-2229 or theresa.kenney@cchmc.org.

    The Biochemistry Core Laboratory is on the Cincinnati Children’s Burnet Campus in Location R, third floor, room R3053.