Zorn Lab

  • Endoderm Patterning

    Shortly after endoderm tissue is formed in the early embryo, it becomes patterned, so that some of it gives rise to the foregut while other regions give rise to the hindgut. The foregut endoderm contains the progenitor cells that will give rise to the liver, pancreas, thyroid and lungs, whereas the hindgut domain gives rise to the intestine.

    This patterning process, which progressively subdivides the endoderm into organ domains, begins during gastrulation and continues throughout early development, ensuring that the organs form in the correct position in the body. The molecular mechanisms that control this are poorly understood, but appear to be mediated by a series of growth factor interactions between the endoderm and surrounding mesoderm. One of the first manifestations of this patterning is the asymmetric expression of genes such the homeobox transcription factor Hhex in the foregut progenitor cells. Hhex is a homeodomain transcription factor that is essential for anterior endoderm and liver development.

    We are trying to understand the molecular mechanisms that pattern the embryonic endoderm into organ domains. Our recent studies have found that different levels of Wnt, BMP and FGF growth factors in different parts of the embryo are a key step in patterning the endoderm into foregut and hindgut domains. Moreover these growth factors are used over and over again with different roles at different times and places in the embryo. We have shown that, shortly after gastrulation Wnt signaling must be repressed in the anterior endoderm by the secreted protein Sfrp5 in order to promote the development of foregut progenitor cells and the liver, lungs and pancreas. In contrast, high levels of Wnt in the posterior promote hindgut progenitors and intestinal differentiation. Using mouse genetics and transgenic frog embryos, which express green fluorescent protein under the control of the hhex promoter, we are characterizing how Wnts, BMP and Nodal growth factors control the complex transcriptional program of foregut development.

    These studies will provide essential information on the genetic pathways controlling the earliest steps in digestive system development. In recent collaborations with the Wells and Shroyer labs these developmental paradigms have been utilized to direct the differentiation of human stem cells into intestinal tissue.

  • Related Publications

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    2011

    Spence JR, Mayhew CN, Rankin SA, Kuhar MF, Vallance JE, Tolle K, Hoskins EE, Kalinichenko VV, Wells SI, Zorn AM, Shroyer NF, Wells JM. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature. 470:105-9. 2011.

    Rankin SA, Kormish J, Jegga, A Kofron M, Zorn AM. A gene regulatory network controlling hhex transcription in the anterior endoderm of the organizer. Dev. Bio.351: 297-310. 2011.

    2008

    Li Y, Rankin SA, Sinner D, Kenney, AP, Krieg PA, Zorn AM. Sfrp5 coordinates foregut specification and morphogenesis by antagonizing both canonical and non-canonical Wnt11 signaling. Genes & Development. 22:3050-63. 2008.

    Other Significant Publications

    McLin VA, Rankin SA, Zorn AM. Repression of Wnt/beta-catenin signaling in the anterior endoderm is essential for liver and pancreas development.Development. 134: 2207-2217. 2007.

    Costa JMB, Mason J, Lee M, Amaya E, Zorn AM. Novel gene expression domains reveal early patterning of the Xenopus endoderm.GEP. 3: 509-519. 2003.

    Zorn AM, Butler K, Gurdon JB. Anterior endomesoderm specification in Xenopus by Wnt/beta-catenin and TGF-beta signalling pathways.Dev Biol 209: 282-297. 1999.


 
  • Serial sections of a Xenopus gastrula.

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    Serial sections of a Xenopus gastrula.
    In situ hybridization to serial sections of a Xenopus gastrula show that hhex is expressed only in the anterior endoderm, in contrast to Sox17, which is expressed in all of the endoderm tissue.
  • Confocal image of a hhex:gfp transgenic Xenopus embryo showing florescent green foregut progenitors cells.

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    Confocal image of a hhex:gfp transgenic Xenopus embryo showing florescent green foregut progenitors cells.

    Confocal image of a hhex:gfp transgenic Xenopus embryo showing florescent green foregut progenitors cells.