Digestive Health Center

  • Inflammatory and Diarrheal Diseases

    Xi (Jason) Jiang, PhD
    Professor
    Department of Pediatrics; Division of Infectious Disease

    Description of Research

    Dr. Jiang’s research focuses on the interaction of Norovirus (NV) with human histo-blood group antigen receptors. He has characterized the receptor binding patterns of fourteen strains of NVs using recombinant virus-like particles (VLPs) generated from baculovirus-infected insect cells and identified eight receptor binding patterns defined by the ABO, Lewis, and secretory blood types of saliva donors. The eight binding patterns are classified into two binding groups: the A/B binding group and the Lewis binding group. A genetic relatedness has been found among strains with similar binding patterns. The molecular basis of the pathogen/receptor interaction has been further elucidated, including mapping of the binding domain and characterization of the binding interface. Based on these data, Dr. Jiang has proposed a model of the receptor/ligand interaction and initiated a study to screen antivirals against NVs by the computer-aided approach. 

    Collaborations and Core Use

    Dr. Jiang collaborates with Dr. Morrow investigating how the glycans found in breast milk serve as a protective measure by inhibiting pathogens from adhering to their receptors in intestinal epithelial cells. Anticipated use of Cores: Integrative Morphology and Gene and Protein Expression Cores.

 
  • Research image.

    click image to enlarge

    Research image.

    Elucidation of the HBGA- binding pocket. From left to right: an electron microscopy image of noroviruses, a single virus-like particle (VLP), a P dimer with indication of the carbohydrate-binding interface (colored region), and the crystal structure of the human histo-blood group antigens (HBGA)-binding interface. The dashed square in each left panel is enlarged in the right panel. The HBGA is indicated by a ring-shaped trisaccharide. Figure from PLoS One, 2010;6: e1000983.