• Development and Digestive Diseases

    Jeffrey A. Whitsett, MD
    Professor
    Co-Director, Perinatal Institute
    Department of Pediatrics; Chief, Section of Neonatology, Perinatal and Pulmonary Biology
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    Description of Research

    Dr. Whitsett’s digestive disease related project focuses on the role of Sox, KLF, and Ets protein in GI development and function. His work has led him to identify genes and processes critical for gastrointestinal development. For example SPDEF is an ETS transcription factor that has been shown to play a role in mucous cell differentiation in the lung. Together with Dr. Shroyer, Dr. Whitsett has discovered that SPDEF controls intestinal mucous (goblet) cell maturation and cell cycle exit. Additionally, Dr. Whitsett is examining the role of the Kruppel family transcription factor Klf5 in embryonic intestinal development with Dr. Shroyer.

    Recently, Dr. Whitsett along with his colleague Dr. Wells established that the extrahepatobiliary system shares a common origin with the ventral pancreas and not the liver. These pancreatobiliary progenitor cells coexpress the transcription factors PDX1 and SOX17 at E8.5 and their segregation into a PDX1+ ventral pancreas and a SOX17+ biliary primordium is Sox17-dependent. Furthermore, they were able to demonstrate that deleting Sox17 at E8.5 resulted in the loss of biliary structures and ectopic pancreatic tissue in the liver bud and common duct. However when they overexpressed Sox17 development of the pancreas was suppressed and there was evidence of ectopic biliary-like tissue throughout the PDX1+ domain.

    Collaborations

    Dr. Whitsett collaborates with Drs. Wells and Zorn on KLF-5 and Sox proteins in GI-gall bladder-pancreas specification. Additionally, he works with Dr. Shroyer examining GI goblet cell differentiation.

  • Research image.

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