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The first area is non-alcoholic fatty liver disease (NAFLD) pathogenesis. We focus on the role of lipotoxicity, reactive oxygen species, inflammatory pathways and endoplasmic reticulum stress in the generation and regulation of the extreme stage of this disease; nonalcoholic steatohepatitis (NASH). Our laboratory has contributed to the field by highlighting the role of fructose in triggering above mentioned oxidative injury and inflammation which results in fibrosis within the liver. We introduced a novel yet simple dietary model of murine NASH wherein the disease is recapitulated with all its essential hepatic histologic features (steatosis, inflammation and fibrosis) in a milieu of obesity and insulin resistance (See Figure 1).
Figure 1 - Non-alcoholic fatty liver disease (NAFLD) pathogenesis.
Our second area of research is the impact of bariatric surgery on non-alcoholic fatty liver disease (NAFLD) and bile acid physiology. In this area of research the laboratory works to understand the mechanism and impact of bariatric surgical procedures such as sleeve gastrectomy and roux-en-Y gastric bypass on nonalcoholic steatohepatitis (NASH) and other co-morbidities of obesity. Our research involves using small animal models of common and experimental bariatric procedures to study the role of bile acids in the improvements seen after bariatric surgery. We began this work by using an experimental bariatric procedure called ileal transposition in rats (see figure 1), but we’ve progressed to murine vertical sleeve gastrectomy (VSG), specifically its effects on intestinal adaptation (see figure 2). Recently, we are also using ileal enteroid cultures (see figure 3) for investigating the mechanistic role of bile acid triggered signaling pathways including the farnesoid x receptor (FXR) and its downstream effectors such as fibroblast growth factor (FGF19).
Figure 1 - Impact of Bariatric Surgery on NAFLD and Bile acid physiology.Figure 2 - Apical Sodium Bile acid Transporter (ASBT) staining of the terminal ileum sections with ASBT antibody (green fluorescence). Stained area was significantly larger in VSG compared to Sham-operated mice.Figure 3 - Ileal enteroid cultures: Intestinal tissue from
C57bl/6 mice was used to grow enteroids. This representative image shows Edu
incorporation (yellow fluorescence) in ileal derived murine enteroid villous
cells suggesting proliferative response.
Associate Medical Director, Liver Transplant ProgramCo-Director, Steatohepatitis CenterAssociate Professor, UC Department of Pediatrics Phone: 513-803-7044Fax: 513-803-2785Email: firstname.lastname@example.org Follow Dr. Kohli on Twitter.
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