James Lab

  • Current Projects

    Quantification of PAO Accumulation in Human Cardiomyopathy Specimens
    In this project we are studying hearts explanted from adults with end stage heart failure due to ischemic cardiomyopathy or dilated cardiomyopathy. Using an antibody that recognizes a PAO conformer, we are able to quantify the amount of myocardium occupied by PAO. Correlations of PAO accumulation with markers of altered intracellular homeostasis, patient age, clinical cardiac status and comorbidities are also focuses of this project.

    Funding: RO1 HL087862-01A1
    Principle Investigator:  Jeffrey Robbins NIH / NHLBI

    Echocardiography of Murine Species
    The Mouse Echocardiography Core is currently supporting a variety of mouse and rat cardiac projects in molecular cardiovascular biology including studies of hypertrophic and dilated cardiomyopathies, congenital cardiac malformations and cardiac valve dysfunction. In addition to adult and juvenile mouse echocardiography, detailed anatomic and functional studies are also performed in fetal rats and mice.

 
  • Confocal imaging of preamyloid oligomer (PAO) staining.

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    Confocal imaging of preamyloid oligomer (PAO) staining.
    Confocal imaging of preamyloid oligomer (PAO) staining. A&B: PAO staining from failing heart in longitudinal (left) and cross-sectional (right) planes. C&D: PAO staining from a nonfailing heart in planes similar to failing sample above. All 600X magnification.
  • Representative images of mouse echocardiography with a 30MHz transducer.

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    Representative images of mouse echocardiography with a 30MHz transducer.
    Representative images of mouse echocardiography with a 30MHz transducer. A: Normal mouse two-dimensional image (left) and corresponding M-mode (right). Left ventricular size and systolic performance are normal. B: Dilated cardiomyopathy mouse, showing left ventricular enlargement and decreased systolic function. C: Hypertrophic cardiomyopathy mouse, showing thickening of the interventricular septum and left ventricular posterior wall as well as decreased systolic function.