Neuromuscular Development Group
How We Collaborate

How We Collaborate

In pursuit of our goal to accelerate research, the Neuromuscular Development Group meets weekly for discussion. These meetings include progress report presentations from trainees in each lab (on a rotational basis). We also meet monthly to specifically discuss research articles published by others which are of direct interest to the group.

In addition to the free exchange of ideas, the Neuromuscular Development Group encourages the exchange of techniques, mouse lines and reagents amongst its members to accelerate their research.

 

Current Collaborations

Satellite Cell Dysregulation in Neuromuscular Contractures

A collaborative effort between the Cornwall and Millay laboratories to investigate the behavior of satellite cells (muscle stem cells) in postnatal muscle growth following neonatal nerve injury involving the exchange of mouse strains and techniques.

Figure 1: Cross-section of muscle fluorescently labeled to detect myonuclei (blue) and muscle stem cells called satellite cells (magenta). Satellite cells mediate muscle growth and repair by myonuclear addition, and reside outside muscle fibers as indicated by the basement membrane marker, Laminin (green). Figure 1 (click image to enlarge): Cross-section of muscle fluorescently labeled to detect myonuclei (blue) and muscle stem cells called satellite cells (magenta). Satellite cells mediate muscle growth and repair by myonuclear addition, and reside outside muscle fibers as indicated by the basement membrane marker, Laminin (green).
Figure 2: Cell engraftment by Green Fluorescent Protein-positive myoblasts into muscle fibers detected 4 weeks following transplantation at 5 days post-neonatal denervation.Figure 2 (click image to enlarge): Cell engraftment by Green Fluorescent Protein-positive myoblasts into muscle fibers detected 4 weeks following transplantation at 5 days post-neonatal denervation.
Neural Circuits Controlling Motor Behaviors in Health and Disease

The Crone laboratory works on a number of projects, including:

  • Characterizing spinal interneurons that mediate corticospinal control of spinal motor circuits.
  • Identifying brainstem and spinal neurons that activate compensatory respiratory muscles following disease or injury.
  • Investigating the role of semaphorin signaling in degeneration of the neuromuscular junction in amyotrophic lateral sclerosis (ALS).
Figure 1: A motor neuron in the cervical spinal cord is fluorescently labeled following injection of adeno-associated virus into muscle.Figure 1 (click image to enlarge): A motor neuron in the cervical spinal cord is fluorescently labeled following injection of adeno-associated virus into muscle.

Figure 2: V2a neurons in the medial reticular formation of the brainstem are fluorescently labeled in transgenic mice. V2a neurons are important for driving motor behaviors such as locomotion and breathing.Figure 2: V2a neurons in the medial reticular formation of the brainstem are fluorescently labeled in transgenic mice. V2a neurons are important for driving motor behaviors such as locomotion and breathing.
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