Zubair M. Ahmed, PhD
is working to identify and characterize genes that control the function of the visual and auditory sense organs of mammals through the study of individuals with genetic disorders like Usher syndrome (USH). Our lab uses molecular biologic and genetic approaches, human and mouse models, as well as heterologous cell culture expression systems. View the Ahmed Lab.
513-636-4718
zubair.ahmed@cchmc.org
Zubair M. Ahmed, PhD
Academic Information
Assistant Professor, UC Department of Pediatrics
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Specialties
Biography
Education and Training
Research fellow: Laboratory of Molecular Genetics, NIDCD, NIH, Rockville, MD, 2003-2009. Postdoctoral fellow: Laboratory of Molecular Genetics, NIDCD, NIH, Rockville, MD, 2002-2003. Pre-doctoral fellow: Laboratory of Molecular Genetics, National institute of Deafness and Other Communication Disorders (NIDCD), National Institutes of Health (NIH), Rockville, MD, 2000-2002.
Publications
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Riazuddin S, Ahmed ZM, Hegde RS, Khan SN, Nasir I, Shaukat U, Riazuddin S, Butman JA, Griffith AJ, Friedman TB, Choi BY. Variable expressivity of FGF3 mutations associated with deafness and LAMM syndrome. BMC Med Genet. 2011 Feb 9;12:21.
Ahmed ZM, Yousaf R, Lee BC, Khan SN, Lee S, Lee K, Husnain T, Rehman AU, Bonneux S, Ansar M, Ahmad W, Leal SM, Gladyshev VN, Belyantseva IA, Van Camp G, Riazuddin S, Friedman TB, Riazuddin S. Functional null mutations of MSRB3 encoding methionine sulfoxide reductase are associated with human deafness DFNB74. Am J Hum Genet. 2011 Jan 7;88(1):19-29. Friedman TB, Schultz JM, Ahmed ZM, Tsilou ET, Brewer CC. Usher syndrome: hearing loss with vision loss. Adv Otorhinolaryngol. 2011;70:56-65. Rehman AU, Morell RJ, Khan SY, Belyantseva IA, Boger ET, Shahzad M, Ahmed ZM, Riazuddin S, Khan SN, Riazuddin S, Friedman TB. Targeted Capture and Next-Generation Sequencing of the 2.9 Megabase DFNB79 Locus Identifies Mutations in C9orf75, Encoding TAPERIN, as a Cause of Nonsyndromic Deafness. Am J Hum Genet. 2010 Mar 12;86(3):378-88. Khan SY, Riazuddin S, Shahzad M, Ahmed N, Zafar AU, Rehman A, Griffith AJ, Ahmed ZM, Riazuddin S, Friedman TB. DFNB79: reincarnation of a nonsyndromic deafness locus on chromosome 9q34.3. Eur J Hum Genet. 2010;18(1):125-9. Schultz JM, Khan SK, Ahmed ZM, Riazuddin S, Waryah AA, Chhatre D, Starost MF, Ploplis B, Buckley S, Velasquez D, Kabra M, Lee K, Hassan MJ, Ali G, Ansar M, Ghosh M, Wilcox ER, Ahmad W, Merlino G, Leal SM, Riazuddin S, Friedman TB, Morell RJ. Noncoding mutations of HGF3 are associated with nonsyndromic recessive deafness, DFNB39. American J Hum Genet. 2009;85(1):25-39. Waryah AM, Rehman A, Ahmed ZM, Bashir ZH, Khan SY, Zafar AU, Riazuddin S, Friedman TB, Riazuddin S. DFNB74, a novel autosomal recessive nonsyndromic hearing impairment locus on chromosome 12q14.2-q15. Clinical Genetics. 2009;76(3):270-5. Khan SY, Riazuddin S, Shahzad M, Ahmed N, Zafar AU, Rehman A, Griffith AJ, Ahmed ZM, Riazuddin S, Friedman TB. DFNB79: reincarnation of a nonsyndromic deafness locus on chromosome 9q34.3. Eur J Hum Genet. 2009. Available online. Riazuddin S, Anwar S, Janssen A, Ahmed ZM, Khan SY, Belyantseva I, Jochen E, Friedman PL, Riazuddin S, Fahlke C, Friedman TB. Molecular basis of DFNB73: mutations of BSND are associated with nonsyndromic deafness or Bartter syndrome. Am J Hum Genet. 2009;85:1-8. Ahmed ZM, Riazuddin S, Khan S, Friedman P, Riazuddin S, Friedman TB. USH1H, a novel locus for type I Usher syndrome, maps to chromosome 15q22-23. Clin Genet. 2009;75:86-91.
Grants
Molecular Genetics of Usher Syndrome Type I. National Institutes of Health. Aug 2009- Jul 2012. #1K99DC009287.
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Nadean L. Brown, PhD
investigates mechanisms of cell fate specification in the mouse retina and lens. Projects focus on the function and regulation of transcription factor and Notch signaling during progenitor cell proliferation, cell cycle exit and differentiation. Visit the Brown Lab.
513-636-1963
nadean.brown@cchmc.org
Nadean L. Brown, PhD
Academic Information
Associate Professor, UC Department of Pediatrics
Associate Professor, UC Department of Ophthalmology
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Specialties
Biography
Education and Training
BA: Southern Illinois University, Biology and Chemistry, Edwardsville, IL, 1982 PhD: University of Wisconsin, Cellular and Molecular Biology, 1994 Postdoctoral Fellow: University of Michigan, Department of Biology, 1995-1996 Postdoctoral Fellow: University of Michigan Medical School, Division of Internal Medicine and Department of Human Genetics, 1996-1999
Publications
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Prasov, L, Brown, NL and Glaser, T. A critical analysis of Atoh7 (Math5) splicing in the developing mouse retina. PLoS One. 2010; Aug 5(8):e12315. Macgregor S, Hewitt AW, Hysi PG, Ruddle JB,Medland SE, Henders AK, Gordon SD, Andrew T, McEvoy B, Sanfilippo PG, Carbonaro F, Tah V, Li YJ, Bennett SL, Craig JE, Montgomery GW, Tran-Viet KN, Brown NL, Spector TD, Martin NG, Young TL, Hammond CJ, Mackey DA. Genome-wide association identifies ATOH7 as a major gene determining human optic nerve size. Human Molecular Genetics. 2010; Apr 15;340(2):490-503.
Hufnagel RB, Le TT, Riesenberg AL, Brown NL. Neurog2 controls the leading edge of neurogenesis in the mammalian retina. Developmental Biology. 2010;340:490-503.
Riesenberg AN, Liu Z, Kopan R, Brown NL. Rbpj cell autonomous regulation of retinal ganglion cell and cone photoreceptor fates in the mouse retina. Journal of Neuroscience. 2009;29(41): 12865-77.
Riesenberg AN, Le TT, Willardson MI, Blackburn DC, Vetter ML, Brown NL. Pax6 regulation of Math5 during mouse retinal neurogenesis. Genesis. 2009;47(3):175-187.
Le TT, Conley K, Brown NL. Jagged1 is necessary for normal mouse lens development. Developmental Biology. 2009;328(1):118-126.
Willardson MI, Suli A, Pan Y, Marsh-Armstrong N, El-Hodiri H, Chen CB, Moore KB, Brown NL, Vetter ML. Temporal regulation of Ath5 gene expression during eye development. Developmental Biology. 2009;326(2):471-481.
Fuhrmann S, Riesenberg AN, Mathiesen AM, Brown EC, Vetter ML, Brown NL. Characterization of a transient TCF/LEF-responsive progenitor population in the embryonic mouse retina. Invest Ophth Vis Sci. 2009;50(1):432-440.
Rowan S, Conley K, Le TT, Donner AL, Maas RL, Brown, NL. Notch signaling is required for mouse lens growth and differentiation. Developmental Biology. 2008; 321(1):111-122.
Blackburn DC*, Conley K*, Platchetzki D, Kempler K, Battelle BA, Brown NL. Isolation and expression of Pax6 and atonal homologues in the American Horseshoe Crab, Limulus polyphemus. Developmental Dynamics. 2008;237(8):2209-2219. Hufnagel RB, Riesenberg AN, Saul SM, Brown NL. Conserved regulation between Math5 and Math1 as revealed by Math5-GFP transgenes. Molecular and Cellular Neuroscience. 2007;36(4):435-488. Book ChaptersBrown N.L. Ganglion cell development: early steps/fate. In Encyclopedia of the Eye Joseph C. Besharse, Reza Dana, and Darlene A. Dartt (eds.). Vol 2. Amsterdam: Elsevier/Academic, 2010. Hufnagel, RB and Brown, NL. Specification of Retinal Cell Types in Comprehensive Developmental Neuroscience John Rubenstein and Pasko Rakic (eds). Elsevier/Academic In Press.
Grants
Cell-Cell Signaling during Mammalian Eye Formation. Principal Investigator. National Institutes of Health. Apr 2008 - Mar 2012. #EY18097. Investigation of Mammalian Retinal Neuron Development. Principal Investigator. National Institutes of Health. Aug 2009 - July 2013. #EY13612.
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Tiffany Cook, PhD
studies cell type specification using the Drosophila eye as a model. The lab is focuses on gene regulation, and uses a combination of developmental genetics and biochemistry to understand the role of various transcription factors during photoreceptor and lens development. Visit the Cook Lab.
513-636-6991
tiffany.cook@cchmc.org
Tiffany Cook, PhD
Academic Information
Assistant Professor, UC Department of Pediatrics
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Specialties
Understanding the molecular basis of eye development; differentiation of color photoreceptor subtypes in the Drosophila retina; cell-specific regulation of opsin gene expression; mechanisms of cell-specific transcriptional activation and repression
Differentiation of color photoreceptor subtypes in the Drosophila retina; cell-specific regulation of opsin gene expression; mechanisms of cell-specific transcriptional activation and repression Visit the Cook Lab.
Biography
Education and Training
BA: Biology, Summa Cum Laude, Phi Beta Kappa 1987-1991
PhD: Biomedical Sciences/Molecular Biology, 1991-1997
Postdoctoral fellowships: Gastroenterology, 1997-1999; Molecular Genetics, 1999-2004
Publications
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Rajkumar P, Rollmann SM, Cook TA, Layne JE. Molecular evidence for color discrimination in the Atlantic sand fiddler crab, Uca pugilator. J Exp Biol. 2010 Dec 15;213(Pt 24):4240-8.
Charlton-Perkins M, Cook TA. Building a fly eye: terminal differentiation events of the retina, corneal lens, and pigmented epithelia. Curr Top Dev Biol. 2010;93:129-73.
Gutzwiller LM, Witt LM, Gresser AL, Burns KA, Cook TA, Gebelein B. Proneural and abdominal Hox inputs synergize to promote sensory organ formation in the Drosophila abdomen. Dev Biol. 2010 Dec 15;348(2):231-43.
McDonald EC, Xie B, Workman M, Charlton-Perkins M, Terrell DA, Reischl J, Wimmer EA, Gebelein BA, Cook TA. Separable transcriptional regulatory domains within Otd control photoreceptor terminal differentiation events. Dev Biol. 2010 Nov 1;347(1):122-32.
Phelan JD, Shroyer NF, Cook T, Gebelein B, Grimes HL. Gfi1-cells and circuits: unraveling transcriptional networks of development and disease. Curr Opin Hematol. 2010 Jul;17(4):300-7.
Witt LM, Gutzwiller LM, Gresser AL, Li-Kroeger D, Cook TA, Gebelein B. Atonal, Senseless, and Abdominal-A regulate rhomboid enhancer activity in abdominal sensory organ precursors. Dev Biol. 2010 Aug 15;344(2):1060-70.
Uhl JD, Cook TA, Gebelein B. Comparing anterior and posterior Hox complex formation reveals guidelines for predicting cis-regulatory elements. Dev Biol. 2010 Jul 1;343(1-2):154-66. Lomberk G, Imoto I, Gebelein B, Urrutia R, and Cook TA. Conservation of the TGF[beta]/labial Homeobox signaling loop in endoderm-derived cells between Drosophila and mammals. Pancreatology. 2010;10(1):74-84.
Maksimovic S, Cook TA, Buschbeck EK. Spatial distribution of opsin-encoding mRNAs in the tiered larval retinas of the sunburst diving beetle Thermonectus marmoratus (Coleoptera: Dytiscidae). J Exp Biol. 2009 Dec;212(Pt 23):3781-94.
Li-Kroeger D, Witt LM, Grimes HL, Cook TA, Gebelein B. Hox and senseless antagonism functions as a molecular switch to regulate EGF secretion in the Drosophila PNS. Dev Cell. 2008 Aug;15(2):298-308.
Grants
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Fumika Hamada, PhD
is working to identify molecular mechanisms of temperature sensation using the fly brain as a model. We will elucidate how temperature stimuli are relayed and represented in the brain and will uncover the temperature-responsive neural circuits using genetic, behavioral and physiological approaches. Visit the Hamada Lab.
513-803-1662
fumika.hamada@cchmc.org
Fumika Hamada, PhD
Academic Information
Assistant Professor, UC Department of Pediatrics
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Specialties
Biography
Education and Training
Postdoctoral Fellow: Brandeis University, 2005 - 2009. Harvard Medical School, 2004 - 2005.
PhD: Tokyo University of Science, Japan, 2004.
BS: Tokyo University of Science, Japan,1999.
Publications
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Sugawara H, Iwabata K, Koshiyama A, Yanai T, Daikuhara Y, Namekawa SH, Hamada FN, Sakaguchi K. Coprinus cinereus Mer3 is required for synaptonemal complex formation during meiosis. Chromosoma. 2009 Feb;118(1):127-39. Hamada FN, Rosenzweig M, Kang K, Pulver SR, Ghezzi A, Jegla TJ, Garrity PA. An internal thermal sensor controlling temperature preference in Drosophila. Nature. 2008 Jul 10;454(7201):217-20. Ishii S, Koshiyama A, Hamada FN, Nara TY, Iwabata K, Sakaguchi K, Namekawa SH. Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1: a molecular link between recombination and chromatin assembly during meiosis. FEBS J. 2008 May;275(9):2032-41. Garcia JL, Navarro IT, Biazzono L, Freire RL, da Silva Guimarães Junior J, Cryssafidis AL, Bugni FM, da Cunha IA, Hamada FN, Dias RC. Protective activity against oocyst shedding in cats vaccinated with crude rhoptry proteins of the Toxoplasma gondii by the intranasal route. Vet Parasitol. 2007 Apr 30;145(3-4):197-206. Hamada FN, Koshiyama A, Namekawa SH, Ishii S, Iwabata K, Sugawara H, Nara TY, Sakaguchi K, Sawado T. Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity. Biochem Biophys Res Commun. 2007 Jan 26;352(4):836-42. Koshiyama A, Hamada FN, Namekawa SH, Iwabata K, Sugawara H, Sakamoto A, Ishizaki T, Sakaguchi K. Sumoylation of a meiosis-specific RecA homolog, Lim15/Dmc1, via interaction with the small ubiquitin-related modifier (SUMO)-conjugating enzyme Ubc9. FEBS J. 2006 Sep;273(17):4003-12. Namekawa SH, Iwabata K, Sugawara H, Hamada FN, Koshiyama A, Chiku H, Kamada T, Sakaguchi K. Knockdown of LIM15/DMC1 in the mushroom Coprinus cinereus by double-stranded RNA-mediated gene silencing. Microbiology. 2005 Nov;151(Pt 11):3669-78. Iwabata K, Koshiyama A, Yamaguchi T, Sugawara H, Hamada FN, Namekawa SH, Ishii S, Ishizaki T, Chiku H, Nara T, Sakaguchi K. DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis. Nucleic Acids Res. 2005 Oct 12;33(18):5809-18. Hamada FN, Park PJ, Gordadze PR, Kuroda MI. Global regulation of X chromosomal genes by the MSL complex in Drosophila melanogaster.Genes Dev. 2005 Oct 1;19(19):2289-94. Namekawa SH, Hamada FN, Sakaguchi K. [Latest frontiers of meiosis research in Coprinus cinereus]. Seikagaku. 2004 Nov;76(11):1450-4. Review.
Grants
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Saima Riazuddin, PhD
is focused on molecular and genetic basis of hearing impairment. For these studies, we utilize large human pedigrees and mutant mouse models. Through genetic screening disease causing mutations are identified in human families. To further understand the underlying cause of deafness in human pedigrees, we generate orthologue mouse models. These mouse models are evaluated for structural, physiological and developmental defects of the inner ear.
513-803-2888
saima.riazuddin@cchmc.org
Saima Riazuddin, PhD
Academic Information
Assistant Professor, UC Department of Pediatrics
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Specialties
Investigating molecular and genetic basis of hearing loss, utilizing human and mouse genetics to identify novel genes and pathways that underlie inherited human hearing impairment.
Biography
Saima Riazuddin, PhD is head of the Laboratory of Molecular Genetics and Assistant Professor in the Division of Pediatric Otolaryngolgoy, Head and Neck Surgery at the Cincinnati Children’s Research Foundation. She holds secondary appointments in the Division of Opthalmology at the Cincinnati Children’s Research Foundation and the Departments of Otolaryngolgoy and Ophthalmology at the University of Cincinnati. Dr. Riazuddin is interested in the molecular and genetic basis of Hearing impairment. She utilizes human and mouse genetics to identify novel genes and pathways that underlie inherited human hearing impairment.
Education and Training
BS: University of the Punjab, Lahore, Pakistan, 1995. MS: Quaid-i-Azam University, Islamabad, Pakistan, 1998. PhD: University of the Punjab, Lahore, Pakistan, 2001.
Publications
View PubMed Publications
Riazuddin S, Ahmed ZM, Hegde RS, Khan SN, Nasir I, Shaukat U, Riazuddin S, Butman JA, Griffith AJ, Friedman TB, Choi BY. Variable expressivity of FGF3 mutations associated with deafness and LAMM syndrome. BMC Med Genet. 2011 Feb 9;12:21. Borck G, Ur Rehman A, Lee K, Pogoda HM, Kakar N, von Ameln S, Grillet N, Hildebrand MS, Ahmed ZM, Nürnberg G, Ansar M, Basit S, Javed Q, Morell RJ, Nasreen N, Shearer AE, Ahmad A, Kahrizi K, Shaikh RS, Ali RA, Khan SN, Goebel I, Meyer NC, Kimberling WJ, Webster JA, Stephan DA, Schiller MR, Bahlo M, Najmabadi H, Gillespie PG, Nürnberg P, Wollnik B, Riazuddin S, Smith RJ, Ahmad W, Müller U, Hammerschmidt M, Friedman TB, Riazuddin S, Leal SM, Ahmad J, Kubisch C. Loss-of-function mutations of ILDR1 cause autosomal-recessive hearing impairment DFNB42. Am J Hum Genet. 2011 Feb 11;88(2):127-37. Ahmed ZM, Yousaf R, Lee BC, Khan SN, Lee S, Lee K, Husnain T, Rehman AU, Bonneux S, Ansar M, Ahmad W, Leal SM, Gladyshev VN, Belyantseva IA, Van Camp G, Riazuddin S, Friedman TB, Riazuddin S. Functional null mutations of MSRB3 encoding methionine sulfoxide reductase are associated with human deafness DFNB74. Am J Hum Genet. 2011 Jan 7;88(1):19-29. Kitajiri S, Sakamoto T, Belyantseva IA, Goodyear RJ, Stepanyan R, Fujiwara I, Bird JE, Riazuddin S, Riazuddin S, Ahmed ZM, Hinshaw JE, Sellers J, Bartles JR, Hammer JA 3rd, Richardson GP, Griffith AJ, Frolenkov GI, Friedman TB. Actin-bundling protein TRIOBP forms resilient rootlets of hair cell stereocilia essential for hearing. Cell. 2010 May 28;141(5):786-98. Hertzano R, Puligilla C, Chan SL, Timothy C, Depireux DA, Ahmed Z, Wolf J, Eisenman DJ, Friedman TB, Riazuddin S, Kelley MW, Strome SE. CD44 is a marker for the outer pillar cells in the early postnatal mouse inner ear. J Assoc Res Otolaryngol. 2010 Sep;11(3):407-18. Rehman AU, Morell RJ, Belyantseva IA, Khan SY, Boger ET, Shahzad M, Ahmed ZM, Riazuddin S, Khan SN, Riazuddin S, Friedman TB. Targeted capture and next-generation sequencing identifies C9orf75, encoding taperin, as the mutated gene in nonsyndromic deafness DFNB79. Am J Hum Genet. 2010 Mar 12;86(3):378-88. Odeh H, Hunker KL, Belyantseva IA, Azaiez H, Avenarius MR, Zheng L, Peters LM, Gagnon LH, Hagiwara N, Skynner MJ, Brilliant MH, Allen ND, Riazuddin S, Johnson KR, Raphael Y, Najmabadi H, Friedman TB, Bartles JR, Smith RJ, Kohrman DC. Mutations in Grxcr1 are the basis for inner ear dysfunction in the pirouette mouse. Am J Hum Genet. 2010 Feb 12;86(2):148-60. Waryah AM, Rehman A, Ahmed ZM, Bashir ZH, Khan SY, Zafar AU, Riazuddin S, Friedman TB, Riazuddin S. DFNB74, a novel autosomal recessive nonsyndromic hearing impairment locus on chromosome 12q14.2-q15. Clin Genet. 2009 Sep;76(3):270-5. Riazuddin S, Anwar S, Fischer M, Ahmed ZM, Khan SY, Janssen AG, Zafar AU, Scholl U, Husnain T, Belyantseva IA, Friedman PL, Riazuddin S, Friedman TB, Fahlke C. Molecular basis of DFNB73: mutations of BSND can cause nonsyndromic deafness or Bartter syndrome. Am J Hum Genet. 2009 Aug;85(2):273-80. Khan SY, Riazuddin S, Shahzad M, Ahmed N, Zafar AU, Rehman AU, Morell RJ, Griffith AJ, Ahmed ZM, Riazuddin S, Friedman TB. DFNB79: reincarnation of a nonsyndromic deafness locus on chromosome 9q34.3. Eur J Hum Genet. 2010 Jan;18(1):125-9.
Grants
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