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The developing pulmonary vasculature was labeled with tomato (Lycopersicon esculentum) lectin injected into umbilical vessels of E18.5 mice. Confocal-aided z stacking of images and 3D reconstruction revealed patterning of the developing pulmonary microvasculature. The anomalous distal vasculature in WlsShhCre consisted of dilated vessels (B), as opposed to the stereotypical microvasculature present in control embryos (A).
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