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Cores

Gene Expression and Sequencing Core

 

Director: Steve Potter, PhD

Microarray Service

Personnel will help with sample preparation, peform the hybridization, and scan the gene chip. For more information visit the Microarray Core Web page.

The core also provides ongoing user support and updates on Affymetrix data analysis software.

Personnel

Microarray Service

Contact: Shawn Smith
Phone: 513-636-0290
Email: shawn.smith@cchmc.org

Strategy and Experimental Design

Contact: Steve Potter, PhD
Phone: 513-636-4850
Email: steven.potter@cchmc.org

Pricing

ServiceCharges for DHC MemberCharges for non DHC Member
Affymetrix
Human: HG U133 Plus 2.0 $212.50 $425
Human Gene 1.0 ST Array $100 $200
Mouse: 230 2.0 $212.50 $425
Mouse Gene 1.0 ST Array will be available soon  
Rat: 230 2.0 $200 $400
Xenopus Genome array $150 $300
Xenopus Tropicalis array $212.50 $425
Zebrafish array $150 $300
Illumina
Sentrix Mouse 6 (6 arrays per chip) $480 (chip); $80/sample $960/chip; $160/sample
Sentrix Human 6 (6 arrays per chip) $480 (chip); $80/sample $960/chip; $160/sample
Re-sequencing processing fee $75 $150
Exon microarray
Human Exon 1.0ST Array $212.50 $425
Mouse Exon 1.0ST Array $212.50 $425
Rat Exon 1.0ST Array $200 $400
Promoter microarray
Human Promoter 1.0R Array $200 $400
Mouse Promoter 1.0R Array $200 $400
Tiling Arrays
Contact Steve Potter for more information    

Note: There are two additional fees. 

  1. $25 fee to test the quality of the RNA to be used for the gene chip analysis
  2. An approximate fee of $200 per sample to prepare the target RNA for hybridization to the microarray

Microarray Discount Policy for DHC Members

Each DHC investigator will receive a 50% discount on all chips with a dollar cap of $2,400 per fiscal year of the DHC grant.

First time microarray core users will have a one time increased dollar cap of $4,000. 

Both dollar cap limits are dependent on the availability of funds.

Note:  If you used the Microarray Service as a DDRDC member you are NOT eligible for the new first time user dollar cap in the DHC. 

Gene Variation and Gene Discovery (GVGD) Service

Personnel provides state-of-the-art genomic and DNA sequencing evaluation for gene characterization, SNP discovery, gene discovery and translational research for gene-based studies. 

Personnel

GVGD Service

Contact: Mehdi Keddache, MS 
Phone: 513-636-2801
Email: mehdi.keddache@cchmc.org

Director of GVGD Service

Contact: Gregory Grabowski, MD
Phone: 513-636-7290
Email: greg.grabowski@cchmc.org

Pricing

DHC members will receive a discount of 25% on services with a dollar cap of $1,000 per fiscal year of the DHC grant as long as funds are available.
To submit your request for services you will need to create an account by going to this website: http://dna.chmcc.org/www
To ensure that you receive the discount be sure to mark the box that you are a DHC member.

ServiceCharges for DHC MemberCharges for non DHC Member
DNA Sequencing
Sequencing reaction from purified plasmid of PCR product $7.50 $10.00
Plasmid miniprep and single direction sequencing $11.25 $15.00
Injection of completed sequencing reactions, includes cleanup $3.75 $5.00
Genotyping
Microsatellite/fragment sizing    
Per 96-well plate, includes dilution and size standard $135.00 $180.00
With analysis and hardcopies $172.50 $230.00
SNP microarrays
(per sample, 16 sample minimum. There is a sample queue available for this service to group with other investigators.)
Illumina Mouse genome linkage panel (1,400+ cross breed markers) $105.00 $140.00
Illumina HumanHap 300 BeadChip $352.50 $470.00
Affymetrix Human Mapping Array 500K  $412.50 $550
Illumina NS-12 BeadChip (13,000+ genome wide coding SNPs) $112.50 $150.00
DNA Extraction
Mouse tails or other laboratory animal tissue, per 96 well plate $135.00 $180
Blood, saliva and bucal swabs/brushes contact Mehdi for pricing  
     

Remember to identify the DHC (P30 DK078392) in all publications resulting from core supported work.

Last updated: May 21, 2008