Gene Expression and Sequencing Core
Director: Steve Potter, PhD
Microarray Service
Personnel will help with sample preparation, peform the hybridization, and scan the gene chip. For more information visit the Microarray Core Web page.
The core also provides ongoing user support and updates on Affymetrix data analysis software.
Personnel
Microarray Service
Contact: Shawn Smith
Phone: 513-636-0290
Email: shawn.smith@cchmc.org
Strategy and Experimental Design
Contact: Steve Potter, PhD
Phone: 513-636-4850
Email: steven.potter@cchmc.org
Pricing
| Service | Charges for DHC Member | Charges for non DHC Member |
|---|
| Affymetrix |
|---|
| Human: HG U133 Plus 2.0 |
$212.50 |
$425 |
| Human Gene 1.0 ST Array |
$100 |
$200 |
| Mouse: 230 2.0 |
$212.50 |
$425 |
| Mouse Gene 1.0 ST Array |
will be available soon |
|
| Rat: 230 2.0 |
$200 |
$400 |
| Xenopus Genome array |
$150 |
$300 |
| Xenopus Tropicalis array |
$212.50 |
$425 |
| Zebrafish array |
$150 |
$300 |
| Illumina |
|---|
| Sentrix Mouse 6 (6 arrays per chip) |
$480 (chip); $80/sample |
$960/chip; $160/sample |
| Sentrix Human 6 (6 arrays per chip) |
$480 (chip); $80/sample |
$960/chip; $160/sample |
| Re-sequencing processing fee |
$75 |
$150 |
| Exon microarray |
|---|
| Human Exon 1.0ST Array |
$212.50 |
$425 |
| Mouse Exon 1.0ST Array |
$212.50 |
$425 |
| Rat Exon 1.0ST Array |
$200 |
$400 |
| Promoter microarray |
|---|
| Human Promoter 1.0R Array |
$200 |
$400 |
| Mouse Promoter 1.0R Array |
$200 |
$400 |
| Tiling Arrays |
|---|
| Contact Steve Potter for more information |
|
|
Note: There are two additional fees.
- $25 fee to test the quality of the RNA to be used for the gene chip analysis
- An approximate fee of $200 per sample to prepare the target RNA for hybridization to the microarray
Microarray Discount Policy for DHC Members
Each DHC investigator will receive a 50% discount on all chips with a dollar cap of $2,400 per fiscal year of the DHC grant.
First time microarray core users will have a one time increased dollar cap of $4,000.
Both dollar cap limits are dependent on the availability of funds.
Note: If you used the Microarray Service as a DDRDC member you are NOT eligible for the new first time user dollar cap in the DHC.
Gene Variation and Gene Discovery (GVGD) Service
Personnel provides state-of-the-art genomic and DNA sequencing evaluation for gene characterization, SNP discovery, gene discovery and translational research for gene-based studies.
Personnel
GVGD Service
Contact: Mehdi Keddache, MS
Phone: 513-636-2801
Email: mehdi.keddache@cchmc.org
Director of GVGD Service
Contact: Gregory Grabowski, MD
Phone: 513-636-7290
Email: greg.grabowski@cchmc.org
Pricing
DHC members will receive a discount of 25% on services with a dollar cap of $1,000 per fiscal year of the DHC grant as long as funds are available.
To submit your request for services you will need to create an account by going to this website: http://dna.chmcc.org/www
To ensure that you receive the discount be sure to mark the box that you are a DHC member.
| Service | Charges for DHC Member | Charges for non DHC Member |
|---|
| DNA Sequencing |
|---|
| Sequencing reaction from purified plasmid of PCR product |
$7.50 |
$10.00 |
| Plasmid miniprep and single direction sequencing |
$11.25 |
$15.00 |
| Injection of completed sequencing reactions, includes cleanup |
$3.75 |
$5.00 |
|
| Genotyping |
|---|
| Microsatellite/fragment sizing |
|
|
| Per 96-well plate, includes dilution and size standard |
$135.00 |
$180.00 |
| With analysis and hardcopies |
$172.50 |
$230.00 |
|
SNP microarrays
(per sample, 16 sample minimum. There is a sample queue available for this service to group with other investigators.) |
|---|
| Illumina Mouse genome linkage panel (1,400+ cross breed markers) |
$105.00 |
$140.00 |
| Illumina HumanHap 300 BeadChip |
$352.50 |
$470.00 |
| Affymetrix Human Mapping Array 500K |
$412.50 |
$550 |
| Illumina NS-12 BeadChip (13,000+ genome wide coding SNPs) |
$112.50 |
$150.00 |
|
| DNA Extraction |
|---|
| Mouse tails or other laboratory animal tissue, per 96 well plate |
$135.00 |
$180 |
| Blood, saliva and bucal swabs/brushes |
contact Mehdi for pricing |
|
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|---|
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Remember to identify the DHC (P30 DK078392) in all publications resulting from core supported work.
Last updated: May 21, 2008
