Gene Expression and Sequencing Core
Director: Steve Potter, PhD
Microarray Service | Gene Variation and Gene Discovery (GVGD) Service
Microarray Service
Personnel will help with sample preparation, peform the hybridization, and scan the gene chip. For more information visit the Microarray Core Web page.
The core also provides ongoing user support and updates on Affymetrix data analysis software.
Personnel
Microarray Service
Contact: Shawn Smith
Phone: 513-636-0290
Email: shawn.smith@cchmc.org
Strategy and Experimental Design
Contact: Steve Potter, PhD
Phone: 513-636-4850
Email: steven.potter@cchmc.org
Pricing
| Service |
Charges for DHC Member |
Charges for non DHC Member |
| Affymetrix |
| Human: U133 Plus 2.0 |
$200.00 |
$400.00 |
| Human Gene 1.0 ST Array |
$87.50 |
$175.00 |
| Mouse: 430 2.0 |
$200.00 |
$400.00 |
| Mouse Gene 1.0 ST Array |
$87.50 |
$175.00 |
| Rat: 230 2.0 |
$187.50 |
$375.00 |
| Rat Gene 1.0 ST Array |
$87.50 |
$175.00 |
| Xenopus Genome array |
$137.50 |
$275.00 |
| Xenopus Laevis 2.0 |
$187.50 |
$375.00 |
| Zebrafish array |
$137.50 |
$275.00 |
| Re-Sequencing processing fee |
$75.00 |
$150.00 |
| Illumina Array |
| MouseWG-6 v2 Expression BeadChip (2 pack) |
$960 (12 samples per pack)
$80/sample |
$1920 (12 samples per pack)
$160/sample |
| HumanWG-6 v3 Expression BeadChip Kit (2 pack) |
$960 (12 samples per pack)
$80/sample |
$1920 (12 samples per pack)
$160/sample |
| HumanHT-12 v3 Expression BeadChip Kit (2 pack) |
$900 (24 samples per pack)
$37.50/sample |
$1800 (24 samples per pack)
$75/sample |
| Affymetrix Exon Microarray |
| Human Exon 1.0ST Array |
$200.00 |
$400.00 |
| Mouse Exon 1.0ST Array |
$200.00 |
$400.00 |
| Rat Exon 1.0ST Array |
$187.50 |
$375 .00 |
Note: There are two additional fees.
- $25 fee to test the quality of the RNA to be used for the gene chip analysis
- A labeling fee per sample to prepare the target RNA for hybridization to the microarray
Microarray Discount Policy for DHC Members
Each Digestive Health Center (DHC) investigator will receive a 50% discount on all chips up to $2,400 per fiscal year of the DHC grant.
First time microarray core users will recieve a 50% discount of all chips up to $4,000.
Both dollar cap limits are dependent on the availability of funds.
Note: If you used the Microarray Service as a DDRDC member you are NOT eligible for the new first time user dollar cap in the DHC.
Remember to acknowledge the use of the Gene Expression and Sequencing core in all publications by stating:
"This project was supported in part by PHS Grant P30 DK078392."
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Gene Variation and Gene Discovery (GVGD) Service
Personnel provides state-of-the-art genomic and DNA sequencing evaluation for gene characterization, SNP discovery, gene discovery and translational research for gene-based studies.
Personnel
GVGD Service
Contact: Mehdi Keddache, MS
Phone: 513-636-2801
Email: mehdi.keddache@cchmc.org
Director of GVGD Service
Contact: Gregory Grabowski, MD
Phone: 513-636-7290
Email: greg.grabowski@cchmc.org
Pricing
Digestive Health Center (DHC) members will receive a discount of 25% on services up to $1,000 per fiscal year of the DHC grant as long as funds are available. Pricing is effective July 1, 2009.
To submit your request for services you will need to create an account by going to this website: http://dna.chmcc.org/www
To ensure that you receive the discount be sure to mark the box that you are a DHC member.
| Service |
Charges for DHC Member |
Charges for non DHC Member |
| DNA Sequencing |
| Sequencing reaction from purified plasmid of PCR product |
$7.50 |
$10.00 |
| Plasmid miniprep and single direction sequencing |
$11.25 |
$15.00 |
| Injection of completed sequencing reactions, includes cleanup |
$3.00 |
$4.00 |
| Genotyping |
| Microsatellite/fragment sizing |
|
|
| Per 96-well plate, includes dilution and size standard |
$135.00 |
$180.00 |
With analysis and hardcopies
|
$172.50 |
$230.00 |
| With PCR |
$221.25 |
$295.00 |
| TaqMan |
|
|
| Per 96-well plate, probes not included |
$56.25 |
$75.00 |
SNP microarrays
(per sample, 16 sample minimum. There is a sample queue available for this service to group with other investigators.) |
| Illumina Mouse genome linkage panel |
$105.00 |
$140.00 |
| Illumina Human660W Quad HD BeadChip |
$367.50 |
$490.00 |
| Illumina HumanOmni1M Quad HD BeadChip |
$408.75 |
$545.00 |
| Illumina Infinium Human Linkage SNP set (6,000+ markers) |
$93.75 |
$125.00 |
| DNA Extraction |
| Mouse tails or other laboratory animal tissue, per 96 well plate |
$112.50 |
$150.00 |
| Blood (processed once a week) |
$11.25 |
$15.00 |
| Saliva (processed twice a month) |
$11.25 |
$15.00 |
| Blood and saliva (immediate provessing) |
$18.75 |
$25.00 |
| Next Generation Sequencing |
| ChIP-seq |
from $1,237.50 / sample |
from $1,650 / sample |
| mRNA-seq |
from $1,237.50 / sample |
from $1,650 / sample |
| microRNA profiling |
from $1,350.00 / sample |
from $1,800 / sample |
| Rare variant detection |
call for pricing |
|
| Insertion site detection |
call for pricing |
|
| Large scale mutation detection |
from $1,237.50 / sample |
from $1,650 / sample |
| Paired-end sequencing |
from $2,100.00 / sample |
from $2,800 / sample |
| Indexed library construction |
from $225.00 / sample |
from $300 / sample |
| Core Instrumentation Access |
Applied Biosystems 3730xl- Fragment sizing injection
Requires Training |
$46.88 |
$62.50 |
Applied Biosystems 3730xl- Sequencing injection
Requires Training |
$46.88 |
$62.50 |
Remember to acknowledge the use of the Gene Expression and Sequencing core in all publications by stating:
"This project was supported in part by PHS Grant P30 DK078392."
Last updated: September 10, 2009
