Sickle cell disease and other hemoglobinopathies; red blood cell physiology; cation transport and volume regulation; hematological problems of the newborn

MD: Duke University School of Medicine, Durham, NC, 1977.

PhD: Physiology and Pharmacology, Duke University Durham, NC, 1977.

Residency: Duke University Medical Center, Durham, NC, 1978-1980.

Fellowship: Children's Hospital, Harvard Medical School, Boston, MA, 1980-1982.

Certification: General Pediatrics, American Academy of Pediatrics, 1983, sub-board in Neonatal/Perinatal Medicine, American Academy of Pediatrics, 1983.

Hammill AM, Risinger MA, Joiner CH, Keddache M, Kalfa TA. Compound heterozygosity for two novel mutations in the erythrocyte protein 4.2 gene causing spherocytosis in a Caucasian patient. Br J Haematol. 2011 Jan 31.

Yang MQ, Laflamme K, Gotea V, Joiner CH, Seidel NE, Wong C, Petrykowska HM, Lichtenberg J, Lee S, Welch L, Gallagher PG, Bodine DM, Elnitski L. Genome-wide detection of a TFIID localization element from an initial human disease mutation.Nucleic Acids Res. 2010 Nov 11.

George A, Pushkaran S, Li L, An X, Zheng Y, Mohandas N, Joiner CH, Kalfa TA. Altered phosphorylation of cytoskeleton proteins in sickle red blood cells: the role of protein kinase C, Rac GTPases, and reactive oxygen species. Blood Cells Mol Dis. 2010 Jun 15;45(1):41-5.

Rayburg M, Kalinyak KA, Towbin AJ, Baker PB, Joiner CH. Fatal bone marrow embolism in a child with hemoglobin SE disease.Am J Hematol. 2010 Mar;85(3):182-4.

Barber LA, Palascak MB, Joiner CH, Franco RS. Aminophospholipid translocase and phospholipid scramblase activities in sickle erythrocyte subpopulations. Br J Haematol. 2009 Aug;146(4):447-55.

Cohen RM, Franco RS, Khera PK, Smith EP, Lindsell CJ, Ciraolo PJ, Palascak MB, Joiner CH. Red cell life span heterogeneity in hematologically normal people is sufficient to alter HbA1c. Blood. 2008 Nov 15;112(10):4284-91.

Khera PK, Joiner CH, Carruthers A, Lindsell CJ, Smith EP, Franco RS, Holmes YR, Cohen RM. Evidence for interindividual heterogeneity in the glucose gradient across the human red blood cell membrane and its relationship to hemoglobin glycation.Diabetes. 2008 Sep;57(9):2445-52.

Joiner CH. Gardos pathway to sickle cell therapies? Blood. 2008 Apr 15;111(8):3918-9.

Lindsell CJ, Franco RS, Smith EP, Joiner CH, Cohen RM. A method for the continuous calculation of the age of labeled red blood cells.Am J Hematol. 2008 Jun;83(6):454-7. Erratum in: Am J Hematol. 2008 Jul;83(7):608.

Roszell NJ, Danton MJ, Jiang M, Witte D, Daugherty C, Grimes T, Girdler B, Anderson KP, Franco RS, Degen JL, Joiner CH. Fibrinogen deficiency, but not plasminogen deficiency, increases mortality synergistically in combination with sickle hemoglobin SAD in transgenic mice.Am J Hematol. 2007 Dec;82(12):1044-8.

Hematology; bone marrow failure; sickle cell anemia; hemoglobinopathy

MD: Temple University, Philadelphia, PA, 1977. 

Residency: Children's Hospital Medical Center, Cincinnati, OH, 1980.

Fellowship: Children's Hospital Medical Center, Cincinnati, OH, 1983.

Certification: Pediatrics, 1984; Pediatric Hematology/Oncology, 1987.

Wang W, Brugnara C, Snyder C, Wynn L, Rogers Z, Kalinyak K, Brown C, Qureshi A, Bigelow C, Neumayr L, Smith-Whitley K, Chui DH, Delahunty M, Woolson R, Steinberg M, Telen M, Kesler K. The effects of hydroxycarbamide and magnesium on haemoglobin SC disease: results of the multi-centre CHAMPS trial.Br J Haematol. 2011 Jan 31.

Mizukawa B, George A, Pushkaran S, Weckbach L, Kalinyak K, Heubi JE, Kalfa TA. Cooperating G6PD mutations associated with severe neonatal hyperbilirubinemia and cholestasis.Pediatr Blood Cancer. 2010 Oct 14.

Rayburg M, Kalinyak KA, Towbin AJ, Baker PB, Joiner CH. Fatal bone marrow embolism in a child with hemoglobin SE disease.Am J Hematol. 2010 Mar;85(3):182-4.

Crosby LE, Modi AC, Lemanek KL, Guilfoyle SM, Kalinyak KA, Mitchell MJ. Perceived barriers to clinic appointments for adolescents with sickle cell disease. J Pediatr Hematol Oncol. 2009 Aug;31(8):571-6.

Grueneich R, Ris MD, Ball W, Kalinyak KA, Noll R, Vannatta K, Wells R. Relationship of structural magnetic resonance imaging, magnetic resonance perfusion, and other disease factors to neuropsychological outcome in sickle cell disease.J Pediatr Psychol. 2004 Mar;29(2):83-92.

Koontz K, Short AD, Kalinyak K, Noll RB. A randomized, controlled pilot trial of a school intervention for children with sickle cell anemia.J Pediatr Psychol. 2004 Jan-Feb;29(1):7-17.

Balasa VV, Kalinyak KA, Bean JA, Stroop D, Gruppo RA. Hyperhomocysteinemia is associated with low plasma pyridoxine levels in children with sickle cell disease.J Pediatr Hematol Oncol. 2002 Jun-Jul;24(5):374-9.

Graumlich SE, Powers SW, Byars KC, Schwarber LA, Mitchell MJ, Kalinyak KA. Multidimensional assessment of pain in pediatric sickle cell disease. J Pediatr Psychol. 2001 Jun;26(4):203-14.

Noll RB, Stith L, Gartstein MA, Ris MD, Grueneich R, Vannatta K, Kalinyak K. Neuropsychological functioning of youths with sickle cell disease: comparison with non-chronically ill peers.J Pediatr Psychol. 2001 Mar;26(2):69-78.

Balasa VV, Gruppo RA, Gartside PS, Kalinyak KA. Correlation of the C677T MTHFR genotype with homocysteine levels in children with sickle cell disease.J Pediatr Hematol Oncol. 1999 Sep-Oct;21(5):397-400.

Signaling in erythrocytes; erythropoiesis; Sickle Cell disease; endothelial cell biology

Visit the Kalfa Lab.

MD: Aristotle University Medical School, Thessaloniki, Greece, 1990.

PhD: Aristotle University Medical School, Thessaloniki, Greece, 1997.

Residency: University Of North Carolina, Chapel Hill, NC, 1999.

Fellowship: Duke University Medical Center, Durham, NC, 2003.

Certification: Hematology / oncology, American Board of Pediatrics, 2004; Pediatrics, American Board of Pediatrics, 2000; ECFMG Certification, 1995.

Licenses: Full and unrestricted medical license (OH Medical Board), 2003-present; full and unrestricted license of medical practice in Greece, 1990-present.

Hammill AM, Risinger MA, Joiner CH, Keddache M, Kalfa TA. Compound heterozygosity for two novel mutations in the erythrocyte protein 4.2 gene causing spherocytosis in a Caucasian patient. Br J Haematol. 2011 Jan 31.

Kalfa TA. Anchoring at an island to relieve stress. Blood. 2011 Jan 20;117(3):748-9.

Mizukawa B, George A, Pushkaran S, Weckbach L, Kalinyak K, Heubi JE, Kalfa TA. Cooperating G6PD mutations associated with severe neonatal hyperbilirubinemia and cholestasis.Pediatr Blood Cancer. 2010 Oct 14.

Konstantinidis DG, George A, Kalfa TA. Rac GTPases in erythroid biology. Transfus Clin Biol. 2010 Sep;17(3):126-30.

George A, Pushkaran S, Li L, An X, Zheng Y, Mohandas N, Joiner CH, Kalfa TA. Altered phosphorylation of cytoskeleton proteins in sickle red blood cells: the role of protein kinase C, Rac GTPases, and reactive oxygen species. Blood Cells Mol Dis. 2010 Jun 15;45(1):41-5.

Kalfa TA, Pushkaran S, Zhang X, Johnson JF, Pan D, Daria D, Geiger H, Cancelas JA, Williams DA, Zheng Y. Rac1 and Rac2 GTPases are necessary for early erythropoietic expansion in the bone marrow but not in the spleen.Haematologica. 2010 Jan;95(1):27-35.

Mulloy JC, Cancelas JA, Filippi MD, Kalfa TA, Guo F, Zheng Y. Rho GTPases in hematopoiesis and hemopathies. Blood. 2010 Feb 4;115(5):936-47.

Wang D, Zhang W, Kalfa TA, Grabowski G, Davies S, Malik P, Pan D. Reprogramming erythroid cells for lysosomal enzyme production leads to visceral and CNS cross-correction in mice with Hurler syndrome.Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19958-63.

Diwan A, Koesters AG, Capella D, Geiger H, Kalfa TA, Dorn GW 2nd. Targeting erythroblast-specific apoptosis in experimental anemia. Apoptosis. 2008 Aug;13(8):1022-30.

Daria D, Filippi MD, Knudsen ES, Faccio R, Li Z, Kalfa T, Geiger H. The retinoblastoma tumor suppressor is a critical intrinsic regulator for hematopoietic stem and progenitor cells under stress. Blood. 2008 Feb 15;111(4):1894-902.

Diwan A, Koesters AG, Odley AM, Pushkaran S, Baines CP, Spike BT, Daria D, Jegga AG, Geiger H, Aronow BJ, Molkentin JD, Macleod KF, Kalfa TA, Dorn GW. Unrestrained erythroblast development in Nix-/- mice reveals a mechanism for apoptotic modulation of erythropoiesis. Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6794-9.

Kalfa TA, Pushkaran S, Mohandas N, Hartwig JH, Fowler VM, Johnson JF, Joiner CH, Williams DA, Zheng Y. Rac GTPases regulate the morphology and deformability of the erythrocyte cytoskeleton. Blood. 2006 Dec 1;108(12):3637-45.

MBBS: University of Delhi, New Delhi, India, 1985.

MD: University of Delhi, New Delhi, India, 1989.

MS: University of Maryland, Baltimore, MD, 1991.

Fellowship: Children's Hospital Los Angeles, University of Southern California, 1995.

Arumugam P, Malik P. Genetic therapy for beta-thalassemia: from the bench to the bedside.Hematology Am Soc Hematol Educ Program. 2010;2010:445-50.

Perumbeti A, Malik P. Therapy for beta-globinopathies: a brief review and determinants for successful and safe correction.Ann N Y Acad Sci. 2010 Aug;1202:36-44. Review.

Perumbeti A, Malik P. Genetic correction of sickle cell anemia and beta-thalassemia: progress and new perspective.ScientificWorldJournal. 2010 Apr 13;10:644-54. Review.

Sundaram N, Tailor A, Mendelsohn L, Wansapura J, Wang X, Higashimoto T, Pauciulo MW, Gottliebson W, Kalra VK, Nichols WC, Kato GJ, Malik P. High levels of placenta growth factor in sickle cell disease promote pulmonary hypertension.Blood. 2010 Jul 8;116(1):109-12.

Wang D, Zhang W, Kalfa TA, Grabowski G, Davies S, Malik P, Pan D. Reprogramming erythroid cells for lysosomal enzyme production leads to visceral and CNS cross-correction in mice with Hurler syndrome.Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19958-63.

Arumugam PI, Urbinati F, Velu CS, Higashimoto T, Grimes HL, Malik P. The 3' region of the chicken hypersensitive site-4 insulator has properties similar to its core and is required for full insulator activity.PLoS One. 2009 Sep 10;4(9):e6995.

Arumugam PI, Higashimoto T, Urbinati F, Modlich U, Nestheide S, Xia P, Fox C, Corsinotti A, Baum C, Malik P. Genotoxic potential of lineage-specific lentivirus vectors carrying the beta-globin locus control region.Mol Ther. 2009 Nov;17(11):1929-37.

Perumbeti A, Higashimoto T, Urbinati F, Franco R, Meiselman HJ, Witte D, Malik P. A novel human gamma-globin gene vector for genetic correction of sickle cell anemia in a humanized sickle mouse model: critical determinants for successful correction.Blood. 2009 Aug 6;114(6):1174-85.

Urbinati F, Arumugam P, Higashimoto T, Perumbeti A, Mitts K, Xia P, Malik P. Mechanism of reduction in titers from lentivirus vectors carrying large inserts in the 3'LTR.Mol Ther. 2009 Sep;17(9):1527-36.

Williams JP, Wu J, Johansson G, Rizvi TA, Miller SC, Geiger H, Malik P, Li W, Mukouyama YS, Cancelas JA, Ratner N. Nf1 mutation expands an EGFR-dependent peripheral nerve progenitor that confers neurofibroma tumorigenic potential.Cell Stem Cell. 2008 Dec 4;3(6):658-69.

Clinical Interests

Sickle cell disease; thalassemia; hemoglobinopathies; disorders of red blood cells; iron overload and iron chelation; chronic transfusion therapy

Research Interests

Novel causes of sickle cell pain; Role of hemoglobin desaturation in the pathogenesis of sickle cell disease; Survival and long-term follow-up of sickle cell disease; Causes and treatment of stroke in sickle cell disease

MD:  University of Texas Southwestern Medical Center, Dallas, TX, 1994.

MS:  University of Texas Southwestern Medical Center, Dallas, TX, 2008.

Residency & Chief Residency:  Children's Medical Center Dallas, Dallas, TX; University of Texas Southwestern Medical Center, Dallas, TX, 1998.

Fellowship:  Children's Medical Center Dallas, Dallas, TX; University of Texas Southwestern Medical Center, Dallas, TX, 2001.

Certifications:  Pediatrics, 1998; Pediatric Hematology-Oncology, 2002.

Quinn CT, Johnson VL, Kim HY, Trachtenberg F, Vogiatzi MG, Kwiatkowski JL, Neufeld EJ, Fung E, Oliveri N, Kirby M, Giardina PJ; for the Thalassemia Clinical Research Network. Renal dysfunction in patients with thalassaemia. Br J Haematol. 2011 Feb 21. doi: 10.1111/j.1365-2141.2010.08477.x.

McCavit TL, Quinn CT, Techasaensiri C, Rogers ZR. Increase in Invasive Streptococcus Pneumoniae Infections in Children with Sickle Cell Disease since Pneumococcal Conjugate Vaccine Licensure. J Pediatr. 2011 Mar;158(3):505-7.

Sobota A, Yamashita R, Xu Y, Trachtenberg F, Kohlbry P, Kleinert DA, Giardina PJ, Kwiatkowski JL, Foote D, Thayalasuthan V, Porter JB, Thompson AA, Schilling L, Quinn CT, Neufeld EJ; Thalassemia Clinical Research Network. Quality of life in thalassemia: a comparison of SF-36 results from the thalassemia longitudinal cohort to reported literature and the US norms. Am J Hematol. 2011 Jan;86(1):92-5.

Mednick L, Yu S, Trachtenberg F, Xu Y, Kleinert DA, Giardina PJ, Kwiatkowski JL, Foote D, Thayalasuthan V, Porter JB, Thompson AA, Schilling L, Quinn CT, Neufeld EJ, Yamashita R; Thalassemia Clinical Research Network. Symptoms of depression and anxiety in patients with thalassemia: prevalence and correlates in the thalassemia longitudinal cohort. Am J Hematol. 2010 Oct;85(10):802-5.

Jordan LC, McKinstry RC 3rd, Kraut MA, Ball WS, Vendt BA, Casella JF, DeBaun MR, Strouse JJ; Silent Infarct Transfusion Trial Investigators. Incidental findings on brain magnetic resonance imaging of children with sickle cell disease. Pediatrics. 2010 Jul;126(1):53-61.

Fung EB, Xu Y, Kwiatkowski JL, Vogiatzi MG, Neufeld E, Olivieri N, Vichinsky EP, Giardina PJ; Thalassemia Clinical Research Network. Relationship between chronic transfusion therapy and body composition in subjects with thalassemia. J Pediatr. 2010 Oct;157(4):641-7, 647.e1-2.

Quinn CT, Rogers ZR, McCavit TL, Buchanan GR. Improved survival of children and adolescents with sickle cell disease. Blood. 2010 Apr 29;115(17):3447-52.

Dowling MM, Lee N, Quinn CT, Rogers ZR, Boger D, Ahmad N, Ramaciotti C, Buchanan GR. Prevalence of intracardiac shunting in children with sickle cell disease and stroke. J Pediatr. 2010 Apr;156(4):645-50.

Ghatpande SS, Choudhary PK, Quinn CT, Goodman SR. In vivo pharmaco-proteomic analysis of hydroxyurea induced changes in the sickle red blood cell membrane proteome. J Proteomics. 2010 Jan 3;73(3):619-26.

Dowling MM, Quinn CT, Rogers ZR, Buchanan GR. Acute silent cerebral infarction in children with sickle cell anemia. Pediatr Blood Cancer. 2010 Mar;54(3):461-4.

Clinical Interests

Adolescents; sickle cell disease; transition; chronic pain; migraines; cultural competence

Research Interests

Community-based research; self-management; quality improvement; sickle cell disease; health disparities

Visit the Crosby Lab.

PsyD: Wright State University, Dayton, Ohio, 1995.

Residency: Cincinnati Children's Hospital Medical Center.

Fellowship: Clinical Psychology, INTERACT Behavioral Healthcare Services Inc, Columbus, OH, 1995-1996.

Hines J, Mitchell M, Crosby L, Johnson A, Valenzuela J, Kalinyak K, Joiner C. Engaging Patients with Sickle Cell Disease and their Families in Disease Education, Research, and Community Awareness. J Prevent Intervent Comm. 2011.

Neal-Barnett A, Stadulis M, Payne MR, Crosby L, Mitchell M, Williams L, Costa CW. In the company of my sisters: Sister circles as an anxiety intervention for professional African American women. J Affect Disord. 2011 Mar;129(1-3):213-8.

Oliver-Carpenter G, Barach I, Crosby LE, Valenzuela J, Mitchell MJ. Disease management, coping, and functional disability in pediatric sickle cell disease. J Natl Med Assoc. 2011 Feb;103(2):131-7.

Lynch-Jordan AM, Kashikar-Zuck S, Crosby LE, Lopez WL, Smolyansky BH, Parkins IS, Luzader CP, Hartman A, Guilfoyle SM, Powers SW. Applying quality improvement methods to implement a measurement system for chronic pain-related disability. J Pediatr Psychol. 2010 Jan-Feb;35(1):32-41.

Brinkman WB, Sherman SN, Zmitrovich AR, Visscher MO, Crosby LE, Phelan KJ, Donovan EF. Parental angst making and revisiting decisions about treatment of attention-deficit/hyperactivity disorder. Pediatrics. 2009 Aug;124(2):580-9.

Crosby LE, Modi AC, Lemanek KL, Guilfoyle SM, Kalinyak KA, Mitchell MJ. Perceived barriers to clinic appointments for adolescents with sickle cell disease. J Pediatr Hematol Oncol. 2009 Aug;31(8):571-6.

 Bolling C, Crosby L, Boles R, Stark L. How pediatricians can improve diet and activity for overweight preschoolers: a qualitative study of parental attitudes. Acad Pediatr. 2009 May-Jun;9(3):172-8.

Modi A, Crosby L, Guilfoyle S, Lemanek K, Witherspoon D, Mitchell M. Barriers to Treatment Adherence for Pediatric Patients with Sickle Cell Disease and their Families. Children’s Health Care. 2009.

Beidel D, Turner S, Sallee R, Ammerman R, Crosby L, Pathak S. SET-C vs. fluoextine in the Treatment of Childhood Social Phobia. J Am Acad Child Adolesc Psych. 2007;46:1622-1632.

Mitchell MJ, Lemanek K, Palermo TM, Crosby LE, Nichols A, Powers SW. Parent perspectives on pain management, coping, and family functioning in pediatric sickle cell disease. Clin Pediatr (Phila). 2007 May;46(4):311-9.