Cancer & Blood Diseases Institute

Clinton H. Joiner, MD, PhD

Title

Director, Hematology Program

Appointment

Professor of Pediatrics, Molecular and Cellular Physiology, University of Cincinnati College of Medicine

Email

clinton.joiner@cchmc.org

Phone

513-636-4541

Fax

513-636-5562

Credentials

MD: Duke University School of Medicine, Durham, NC, 1977.
PhD: Physiology and Pharmacology, Duke University Durham, NC, 1977.
Residency: Pediatrics, Duke University Medical Center, Durham, NC, 1978-1980.
Fellowship: Medicine/Neonatology, Children's Hospital, Harvard Medical School, Boston, MA, 1980-1982.
Certification: General Pediatrics, American Academy of Pediatrics, 1983, sub-board in Neonatal/Perinatal Medicine, American Academy of Pediatrics, 1983.

Awards and Honors

Fellow, American Academy of Pediatrics Medical
Scientific Advisory Committee, Sickle Cell Disease Association of America
Ad Hoc member, Erythrocyte, Leukocyte Biology Study Section, National Heart, Lung, and Blood Institute (NHLBI)
Chair, Steering Committe of the National Institute of Health (NIH) Comprehensive Sickle Cell Centers
Editorial Board, American Journal of Hematology
Hemoglobinopathies Coordinating Committee, National Heart Lung and Blood Institute
Medical and Scientific Advisory Committee, Sickle Cell Disease Association of America

Research Grants and Contracts

National Heart Lung and Blood Institute: Cincinnati Comprehensive Sickle Cell Center U54 HL070871-06; 6/01/2008 - 5/31/2012. C. H. Joiner, Program Director.

Ohio Department of Health: Cincinnati Sickle Cell Disease Project; 7/01/1996 - 2004, C.H. Joiner, Program Director.

Health Resources and Services Administration, United States Public Health Service. H46MC009233-01. Cincinnati Sickle Cell Newborn Screening Network. 6/02/2008 - 5/31/2011; Lisa Shook, P.I, Co-Investigator: C. H. Joiner, Co-Investigator.

Publications, Most Recent

Connect to Clinton Joiner's publications on PubMed

Rinehart J, Maksimova YD, Tanis JE, Stone KL, Hodson CA, Zhang J, Risinger M, Pan W, Wu D, Colangelo CM, Forbush B, Joiner CH, Gulcicek EE, Gallagher PG, Lifton RP. Quantitative phosphoproteomics identifies regulatory sites in K-Cl cotransporters: Implications for control of cell volume and neuronal excitability. Cell 2009, in press.

Barber LA, Palascak MB, Joiner CH, Franco RS. Aminophospholipid Traslocase and Phospholipid Scramblase Activities in Sickle Erythrocyte Subpopulations.Brit J Haematol, 2009, In press.

Cohen RM, Franco RS, Khera PK, Smith EP, Lindsell CJ, Ciraolo PJ, Palascak MB, Joiner CH. Red cell lifespan heterogeneity in hematologically normal people is sufficient to alter HbA1c. Blood 112(10):4284-91, 2008.

Khera PK, Joiner CH, Carruthers A, Lindsell CJ, Smith EP, Franco RS, Homes YR, Cohen RM. Evidence for inter-individual heterogeneity in the glucose gradient across the human RBC membrane and its relationship to hemoglobin glycation. Diabetes 57(9):2445-5, 2008.

Lindsell CJ, Franco RS, Smith EP, Joiner CH, Cohen RM. A method for the continuous calculation of the age of labeled red blood cells. Am J Hematol 83(6):454-7, 2008. Erratumin: Am J Hematol 2008 July;83(7):608.

Roszell NJ, Danton MJ, Jiang M, Witte D, Daughtery C, Grimes T, Girdler B, Anderson KP, Franco RS, Degen JL, Joiner CH. Fibrinogen deficiency, but not plasminogen deficiency, increases mortality synergistically in combination with sickle hemoglobin SAD in transgenic mice. Am J Hematol 82(12):1044-8, 2007.

Joiner, CH.; Rettig, RK.; Jiang, M.; Risinger, M.; Franco, RS.: Urea stimulation of KCl cotransport induces abnormal volume reduction in sickle reticulocytes. Blood, 109(4):1728-35, 2007.

Goodman, SR.; Joiner, CH.: Damage to the RBC Membrane in Sickle Cell Disease. In The Renaissance of Sickle Cell Disease Research in the Human Genome Era, B Pace, Ed. Imperial College Press, UK, 2006.

Kalfa, TA.; Pushkaran, S.; Narla, M.; Hartwig, JH; Johnson, J; Joiner, C.H.; Williams, DA.; Zheng, Y.: Rac GTPases regulate the morphology and deformability of the erythrocyte cytoskeleton. Blood, 108(12):3637-45, 2006.

Franco, RS.; Yasin Z.; Palascak, MB.; Ciraolo, P.; Joiner, CH.; Rucknagel, D.L.: Effect of Fetal Hemoglobin on the Survival Characteristics of Sickle Cell. Blood, 108(3):1073-6, 2006.

Crable, SC.; Hammond, SM.; Papes R.; Rettig, RK.; Zhou, GP.; Gallagher, PG.; Joiner, CH.; Anderson, KP.: Multiple isoforms of the KCI cotransporter are expressed in sickle and normal erythroid cells. Exp. Hematology, 33:624-31, 2005.

Joiner, CH, RK Rettig, M Jiang, and RS Franco. KCl Cotransport Mediates Abnormal, Sulfhydryl-Dependent Volume Regulation in Sickle Reticulocytes. Blood, 2004 Nov 1;104(9):2954-60.

Cohen, RM, RS Franco, and CH Joiner. Is poor glycemic control associated with reduced red blood cell lifespan? Invited Editorial, Diabetes Care, 2004 Apr;27(4):1013-4.

Yasin, Z, S Witting, MB Palascak, CH Joiner, DL Rucknagel, and RS Franco. Phosphatidylserine externalization in sickle red blood cells: associations with cell age, density, and hemoglobin F. Blood, 2003 Jul 1;102(1):365-70.

Merciris, P, WJ Claussen, CH Joiner, and F Giraud. Regulation of K-Cl Cotransport by Syk and Src protein tyrosine kinase. Eur.J.Physiol, 446:232-238, 2003.

Cohen, RM, YR Holmes, TC Chenier, and CH Joiner. Discordance between HbA1c and Fructosamine: Evidence for a glycosylation gap and its relation to diabetic retinopathy. Diabetes Care, 26:163-7, 2003.

JD Holtzclaw, M Jiang, Z Yasin, CH Joiner, and RS Franco. Rehydration of high-density sickle erythrocytes in vitro. Blood, 100:3017-3025, 2002.

Professional Organization Memberships

Sigma XI
American Physiological Society
American Society for Hematology
American Academy of Pediatrics
Cincinnati Pediatric Society
Society of General Physiologists
American Pediatric Society
American Society for Pediatric Hematology/Oncology

Abstracts

Joiner CH, Risinger M, Rinehart J, Crable S, Ottlinger A, Winkelmann R, Pan D, Huebner C, Gallagher PG. Structural Interactions of KCl Cotransport Proteins KCC1 and KCC3 in Sickle and Normal Erythrocyte Membranes Have Potential Functional Implications. Oral presentation at National Sickle Cell Disease Meeting, Fort Lauderdale, FL, February 2009.

Kalfa TA, Pushkaran S, An X, Collias N, Joiner CH, Mohandas N, Zheng Y. Sickle red blood cells haev increased phosphorylation of adducin and increased ROS production mediated by signaling pathways involving Rac CTPases. Oral presentation at National Sickle Cell Diease Meeting, Fort Lauderdale, FL, February 2009.

Barber LA, Palascak MB, Joiner CH, Franco RS. Red Blood Cell Scramblase Activition is Induced by Phorbol Ester and Prevented by Inhibition of Protein Kinase C. Poster Presentation at American Society of Hematology National Meeting, San Francisco, CA, December 2008.

Perumbeti A, Higashimoto T, Urbinati F, Lauderback K, Loberg A, Estevez-Pagani G, Meiselman H, Franco R, Witte D, Joiner CH, Malik P. Correlation of Sickle Cell Anemia with Globin Gene Delivered by Lentivirus Vector in the Setting of Myeloablative or Reduced Intensity Conditioning, and Establishing Critical Determinants for Successful Gene Therapy for Sickle Cell Disease, Poster Presentation at American Society of Hematology National Meeting, San Francisco, CA, December 2008.

Risinger M, Rinehart J, Crable S, Ottlinger A, Winkelmann R, Pan D, Huebner C, Gallagher PG, Joiner CH. Structural and Functional Interactions of KCl Cotransport Proteins KCC1 and KCC3 in Sickle and Normal Erythrocyte Membranes. Poster Presentation at American Society of Hematology National Meeting, San Francisco CA, December 2008.

Crable S, Joiner CH, Gallagher PG. A GC box element is critical for transcriptional regulation of the K-Cl cotransporter isoform KCC3a in hematopoietic cells. Poster Presentation at American Society of Hematology National Meeting, Atlanta, GA, December 2007.

Pan D, Kalfa T, Wang D, Risinger M, Crable S, Ciraolo P, Franco RS, Joiner CH. KCl Cotransporter Genes KCC1, KCC3a, and KCC4 are Expressed in Human Erythroid Differentiation. Poster Presentation at American Society of Hematology National Meeting, Atlanta, GA, December 2007.

Crable S, Joiner CH, Gallagher PG. Novel Transcripts of KCl Cotransporter-3 (KCC3) gene in erythroid cells are derived from alternate splicing within exon 1. Platform presented at National Sickle Cell Meetings, Washington, September 2007.

Arnold L, Palsacek M, Ciraolo P, Joiner CH, Franco RS. Aminophospholipid Translocase Activity is Markedly Decreased in Dehydrated Sickle Red Blood Cells. Platform presented at National Sickle Cell Meetings, Washington, September 2007.

Pan D, Wang D, Crable S, Risinger M, Franco RS, Joiner CH. Expressional profile of KCl cotransporters in human erythroid differentiation. Platform presented at National Sickle Cell Meetings, Washington, September 2007.

Joiner CH, R Papes, S Crable, D Pan, and DB Mount: Functional Comparison of Red Cell KCl Cotransporter isoforms, KCC1, KCC3, and KCC4. Presented at American Society of Hematology, Orlando, FL; December 2006.

Joiner CH, S Crable, and PG Gallagher: Alternative Splicing within Exon 1 of the KCl Cotransporter-3 (KCC3) Gene Results in Novel Transcripts in Erythroid Cells. Presented at American Society of Hematology, Orlando, FL; December 2006.

Joiner CH, R Papes, M Jiang, and S Crable: Kinetic characteristics of KCl Cotransporter isoforms, KCC1, KCC3, and KCC4, expressed in HEK 293 cells. Presented at National Sickle Cell Meetings, Memphis, TN; April 2006.

CH Joiner, RK Rettig, M Jiang, and RS Franco: KCl Cotransport Mediates Abnormal Volume Regulation in Sickle Reticulocytes. Presented at National Sickle Cell Meetings, Memphis, TN; April 2006.

CH Joiner, S Crable, K Anderson, S Hammond, and P Gallagher: Expression of a KCl Cotransporter (KCC1) Splice Variant is Suppressted by TNFa and NF-kB in Erytroid Cells. Presented at National Sickle Cell Meetings, Memphis, TN; April 2006.

CH Joiner, RK Rettig, M Palascak, A Sheriff, RM Cohen, and RS Franco: Mature Sickle Cell and Normal Red Cells Exhibit Regulatory Volume Decrease Activated by Urea and Mediated by KCI Cotransport. Presented at American Society of Hematology, Atlanta, GA; December 2005.

CH Joiner: Volume Regulation in Sickle and Normal Reticulocytes. Presented at the Red Cell Club Symposium, University of Illinois - Chicago; September 2005.

RS Franco, M Palascak, P Ciaralo, K Rettig, Z Yasin, and CH Joiner: Sickle Cell Patients with a Higher Percentage of HbF-Containing RBC (F Cells) Have Shorter Survival of RBC that Lack HbF. Presented at American Society of Hematology, San Diego, CA; December 2004.

CH Joiner, K Rettig, M Jiang, and RS Franco: Activation of KCI Cotransport by Urea Induces Dehydration in Both Sickle and Normal Reticulocytes. Presented at American Society of Hematology, San Diego, CA; December 2004.

CH Joiner, M.D., PhD., Kirk Rettig, B.S., Maorong Jiang, M.D., Robert S. Franco, PhD., (Oral presentation) KCI Cotransport Mediates Abnormal Suflhydryl-Dependent Regulatory Volume Decrease In Sickle Reticulocytes. National Sickle Cell Meeting, Los Angeles, CA., April, 2004.

Lori E. Crosby, PhD., Julia Bloom, B.S., Monica Mitchell, PhD., Patricia Pearl, Annette Lavendar, MSN, FNP, Patrick Kelly, M.D., CH Joiner, M.D., PhD., (Oral presentation) Healthbeat: Health Beliefs and Attitudes of Teens and Young Adults with SCD. National Sickle Cell Meeting, Los Angeles, CA., April, 2004.

Pamela Jenkins, M.S., LSW, Lori E. Crosby, PhD., Belinda B.S. LSW, Nona Calhoun-Mumia, M.S., CHES, Reginald Willis, B.S., CH Joiner, M.D. PhD., (Oral presentation) Y Stress, Eustress, Know Stress Parent Support Group. National Sickle Cell Meeting, Los Angeles, CA., April, 2004.

CH Joiner, M.D., PhD., Kirk Rettig, B.S., Maorong Jiang, M.D., Robert S. Franco, PhD., (Oral presentation) Sickle Cells Exhibit Abnormal, Sulfhydryl-Dependent KCI Cotransport Activation by Acid pH and Urea, But Normal Activation by Cell Swelling. National Sickle Cell Meeting, Los Angeles, CA., April, 2004.

Robert S. Franco, PhD., PeterCiraolo, M.S., Mary B. Palascak, B.S., CH Joiner, M.D., PhD., Dense Sickle Cells Contain Decreased Amounts of the Calpain Inhibitor Calpastatin. (Oral presentation), National Sickle Cell Meeting, Los Angeles, CA., April, 2004.

Monica Mitchell, PhD., Kathy Lemanek, PhD., Tonya Palermo, PhD., Lori Crosby, PhD., .Julia Bloom, B.A., Janelle Hines, B.A., CH Joiner, M.D., PhD,. Scott Powers, PhD., Living with Sickle Cell Disease: Coping and Resiliency in Patients, Parents and Siblings. (Poster presentation), National Sickle Cell Meeting, Los Angeles, CA., April, 2004.

CH Joiner, RK Rettig, M Jiang, RS Franco. KCl Cotransport Regulation by Cell Volume, pH and Urea is Abnormal in Sickle Red Blood Cells. Presented at American Society of Hematology, San Diego, December, 2003.

CH Joiner, M.D., Ph.D., R.K. Rettig, B.S., and R.S. Franco, Ph.D. The Volume Set Point of KCl Cotransport in Sickle and Normal Reticulocytes: Effects of Cell Age and Sulfhydryl Reduction. (Oral presentation), National Sickle Cell Meetings, Washington DC, September, 2002.

R.S. Franco, Ph.D., Z. Yasin, M.D., CH Joiner, M.D., Ph.D., and D.L. Rucknagel, M.D., Ph.D. High Sodium Sickle Cells from the Low Density Fraction Have a Very Short In Vivo Survival. Oral presentation, National Sickle Cell Meetings, Washington DC, September, 2002.

K.P. Anderson, Ph.D., S.C. Crable, M.S., A. Hull, B.S. and CH Joiner, M.D., Ph.D., Expression of Multiple KCl Cotransport Isoforms in Sickle and Normal Erythroid Cells. Oral presentation, National Sickle Cell Meetings, Washington DC, September, 2002.

R.S. Franco, Ph.D., J.D. Holtzclaw, Ph.D., and CH Joiner, M.D., Ph.D. Sickle Cell Dehydration Revisited: Evidence for Pathological Rehydration of Dense Cells. (Poster presentation), National Sickle Cell Meetings, Washington DC, September, 2002.

Special Interests

Sickle cell disease and other hemoglobinopathies; red blood cell physiology; cation transport and volume regulation; hematological problems of the newborn