Ward R. Rice, MD, PhD
Title
Director, Neonatology Fellowship Training Program; and Director, Newborn Services, St. Elizabeth Medical Center
Appointment
Professor of Pediatrics
Email
ward.rice@cchmc.org
Phone
513-636-7368
Bio
Pulmonary alveolar Type II cells are vital for normal lung function since they synthesize and secrete surfactant and serve as progenitor cells for the alveolus following lung injury. The laboratory has recently developed methodology to transfect isolated Type II cells utilizing HVJ envelopes, allowing introduction of siRNA and cDNA into primary cultures of Type II cells. This methodology will now be utilized to explore factors regulating secretion of lamellar bodies, and more specifically, secretion of Surfactant Protein C.
The methodology utilized in the laboratory includes generation of multiple transgenic mouse lines, techniques for molecular morphology, tissue culture, cell preparation, and lung physiology as well as standard techniques of molecular biology including Northern, Western, Southern blotting, RT-PCR, cloning and library screening.
Credentials
MD: University of Chicago, 1978.
PhD: University of Chicago, 1976.
Publications, Most Recent
View Ward Rice's publications at Cincinnati Children's as listed by PubMed.
Geary C, Akinbi H, Korfhagen T, Fabre J-E, Boucher R, Koller B, Rice W. Increased susceptibility to Pseudomonas aeruginosa in purinergic receptor knockout mice.Am J Physiol (Lung Cell Mol Physiol) 2005;289:L890-5.
Nesslein LL, Melton KR, Ikegami M, Na C-L, Wert SE, Rice WR, Whitsett JA, Weaver TE. Partial SP-B deficiency perturbs lung function and causes airspace abnormalities.Am J Physiol (Lung Cell Mol Physiol) 2005;288:1154-61.
Uneo T, Linder S, Na C-L, Rice WR, Johansson J, Weaver TE. Processing of pulmonary surfactant protein B by Napsin and Cathepsin H.J Biol Chem 2004;279:16178-84.
Special Interests
Regulation of lung alveolar cell development; surfactant secretion; alternative therapies for cystic fibrosis
Related Areas
This person works in these other areas at Cincinnati Children's Hospital Medical Center:
