Research Flow Cytometry Core

Research Flow Cytometry Core

Education & Training

Online Training - Introduction to Flow Cytometry and RFCC Policies and Procedures

New users need to complete two classes prior to hands-on training on BD FACSCanto and/or Fortessa. The Introduction to Flow Cytometry is a 2 hour class covering instrument components, analysis, compensation and experimental basics. The RFCC Policies and Procedures is a 30 minute class. If you have already been trained on one of our other instruments, please let us know as you don’t need to take the Introduction to Flow Cytometry class again.

For Cincinnati Children's users, register for those classes (RFCC Course 1 and RFCC Course 2) on the ELM by going to CenterLink>MyHub>MyLearning. When completed, you will receive a confirmation email from ELM for each course.

For external users, listen to the two classes below and take the quiz associated:

Hands-On Training

After completing the classes, users must submit a completed registration form to schedule a hands-on training.  Forms can be submitted via email to Sarah Croswell or can be dropped off at R5503. Cincinnati Children’s users will also need to submit the two confirmation emails from ELM.

The hands-on training is 2 hours for the first instrument. If you want to be trained on BD FACSCanto and Fortessa, a 30 minutes extra training will be needed for the second instrument.

You MUST have also access to Stratocore for scheduling and billing. To create a Stratocore account please go to this link. To add a budget number, request it by going under your profile tab in Stratocore.

Online Training - Introduction to Flow Cytometry

New users need to complete the Introduction to Flow Cytometry class prior to the classroom training and the hands-on training on the Aurora. The Introduction to Flow Cytometry is a 2 hour class covering instrument components, analysis, compensation and experimental basics. If you have already been trained on one of our other instruments, please let us know as you don’t need to take this class again.

For Cincinnati Children's users, register for the Introduction to Flow Cytometry class (RFCC Course 1) on the ELM by going to CenterLink>MyHub>MyLearning. When completed, you will received a confirmation email from ELM for the class.

For external users, listen to the class below and take the quiz associated

Classroom Training - Spectral Flow Cytometry

The classroom for Spectral Flow Cytometry has been suspended until further notice. Please contact Celine Silva Lages if you need a training. We will share a video with you and schedule a 30 minute meeting to go over any question you may have before scheduling the hands-on training.

Hands-On Training

After completing the class, users must submit a completed registration form to schedule a 2 hour hands-on training session.  Forms can be submitted via email to Sarah Croswell or can be dropped off at R5503.

You MUST also have access to Stratocore for scheduling and billing. To create a Stratocore account please go to this link. (Hyperlink to https://ppms.us/cchmc/areq/?pf=27). To add a budget number, request it by going under your profile tab in Stratocore.

Online Training - Introduction to Flow Cytometry

New users need to complete the Introduction to Flow Cytometry class prior to hands-on training on the ImageStream. The Introduction to Flow Cytometry is a 2 hour class covering instrument components, analysis, compensation and experimental basics. If you have already been trained on one of our other instruments, please let us know as you don’t need to take this class again.

For Cincinnati Children's users, register for those classes (RFCC Course 1) on the ELM by going to CenterLink>MyHub>MyLearning. When completed, you will received a confirmation email from ELM for the class.

For external users, listen to the class below and take the quiz associated

Hands-On Training

After completing the class, users must submit a completed registration form to schedule a 2 hour hands-on training session. Forms can be submitted via email to Sarah Croswell or can be dropped off at R5503.

You MUST also have access to Stratocore for scheduling and billing. To create a Stratocore account please go to this link. (Hyperlink to https://ppms.us/cchmc/areq/?pf=27). To add a budget number, request it by going under your profile tab in Stratocore.

Staff-Operated Sorting

Prior to using our cell sorting services, new users need to have a Stratocore account and should read the Cell Sorting Guidelines and schedule a consultation with Sherry Thornton or Celine Silva Lages to discuss your experimental needs.

User-Operated Sorting

For those who would like to sort independently, experienced users can be trained on the SH800S, the MA900 and the BigFoot (24/7 access), or FACSAria II and FACSAria Fusion (after-hours access only). Potential trainees must be permanent employees, have good knowledge of flow cytometry, and be a regular cell sorting client. Please contact Sherry Thornton or Celine Silva Lages for more information.

The Ohio River Valley Cytometry Association (ORVCA) and the Research Flow Cytometry Core (RFCC) organize monthly meetings featuring lectures to educate and inform the local community on applications and products used in cytometry.  Email notifications are sent to users via the Research Flow Core email list and to non-RFCC users via the ORVCA email list.

Each year ORVCA and the RFCC organize the Imaging and Cytometry Research Day to bring education and awareness to the scientific community on cytometric applications and developing technologies.  Please visit the ORVCA website for more details.  For updates, sign up for the ORVCA email list.