The core offers multiple target amplification protocols for the preparation of biotin-labeled target. The following table indicates which protocols are available based upon one’s preference of array type. Furthermore, when selecting a protocol one must consider both the quality and quantity of the starting RNA.

Please contact us for fee information.

Array Types

Amplification Protocols and
Labeling Fee Per Sample

Minimum RNA Quality

Amount of RNA

Affymetrix
Gene and
Exon Arrays

Ambion® WT Expression

Intact

100 - 300 ng

 

Ovation Pico WTA
+WT Ovation Exon Module     

Intact or degraded

1 - 20 ng

 

WT-Ovation FFPE System
+ WT Ovation Exon Module

Severely degraded

50 ng of total FFPE RNA

Illumina
BeadChip

Epicentre TargetAmp 1-Round System

Intact

300 - 500 ng

3’  Affymetrix
Expression
Arrays

Genechip 3’ IVT Express Kit

Intact

100 - 300 ng

 

Ovation RNA Amplification V2

Intact

5 - 20 ng

 

Ovation Pico WTA

Intact or degraded

1 - 20 ng

 

WT-Ovation FFPE System

Severely degraded

50 ng of total FFPE RNA

 

Epicentre TargetAmp 1-Round System

Intact

300 - 500 ng