The various species of myxomycetes and basidiomycetes are located in the outdoor environment (local woods, fields, mulch beds, lawns). They are identified using field guides, and the identity of each species is confirmed by an expert. The spores are collected if possible. For very small species of myxomycetes, the entire fruiting body is collected. An allergen extract is made from the gathered material and is filter sterilized, stabilized with 50% glycerin, and stored in sterile vials in a refrigerator. I am still working on refining the methods for making the extracts and plan to also conduct studies to identify the allergenic content of each extract.
Steven L. Stephenson, PhD
Department of Biological Sciences
University of Arkansas
Fayetteville, Arkansas 72701
Dr. Stephenson is a world-renowned expert on myxomycetes and wrote the guidebook that I used for the collection of myxomycete specimens. He also confirmed the identification of the myxomycete species that were studied.
Atin Adhikari, PhD
University of Cincinnati
Cincinnati, OH 45229
Dr. Adhikari is a mycologist. He confirmed the identity of the basidiomycete specimens that were studied.