Pulmonary Function Test: Diffusion Test

The Division of Pulmonary Medicine at Cincinnati Children's performs diffusion tests.

Diffusion tests measure gas transfer from the lungs into the bloodstream. Lung disease can cause changes in this measurement.

Diffusion is measured by having your child place padded nose clips on his or her nose and placing the mouth around a clean, filtered mouthpiece. The mouthpiece is connected to a computer.

Your child will be instructed to breathe normally, exhale fully, take a large quick breath in of a mixture of gases, hold for 10 seconds and then fully exhale. 

Diffusion tests are preformed with other tests and could take up to one hour.

Avoid the following activities:

  • Smoking 24 hours before the test
  • Exercising within one hour of testing
  • Eating two hours before testing

There are no side effects from diffusion testing, and your child may resume normal activities after testing.

The mixture of gases includes a small amount of carbon monoxide that is safe for children of any age. 

Last Updated 08/2013

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Diffusion test video.