24-Hour Urine Collection

When your child's healthcare provider orders a 24-hour urine collection, you will help your child collect his or her urine for a full 24 hours. If the collection starts at 8 am Monday, it is complete at 8 am Tuesday.

Choose a time that is best for you and your child.

  • Plastic container for collection
  • Urine "hat" for toilet (females)
  • Funnel (females)
  1. Have your child urinate at the start time (example: 8 am Monday). Do not save this urine.
  2. You have now started your child's 24-hour urine collection. This is your child's start time. Write your child's name, the date and this starting time on the card attached to the urine container.  
  3. For the next 24 hours, collect all your child's urine in the urine container. Save all urine throughout the 24-hour period.
    • The urine should not be mixed with bowel movements. If urine and bowel movements occur together in the urine hat, you must restart the 24-hour urine collection.      
    • If your child is a female, place the urine "hat" inside the toilet under the lid and have her urinate into this hat. Be sure not to discard toilet tissue in the hat. Using the funnel, pour the urine into the urine container. Keep the urine container in the refrigerator.
    • If your child is a male, have him urinate directly into the urine container. Keep the urine container in the refrigerator.
  4. Twenty-four hours after the collection is begun (example: 8 am Tuesday), have your child empty his or her bladder and add this urine to the container. Try to get your child to urinate as closely as possible to the end of the 24-hour time period. This is your child's stop time. Write the date and this stop time on the card attached to the urine container. No more urine is needed.
  5. Keep the specimen refrigerated until taking the container to the hospital as directed by your child's healthcare provider.

Note for females: Blood in the urine can affect the results of some laboratory tests. If your child is menstruating, ask your child's healthcare provider whether you need to postpone the urine collection.

Last Updated 09/2012