Urine Culture: Male Adolescent

A urine culture is a test that is done to see if there are bacteria (germs) that cause infection in the urine. A "clean catch" urine specimen (one that has no outside bacteria in it) is necessary for an accurate urine culture.

For the specimen to be "clean," careful cleansing of the area is important.

  • Three soapy wipes for cleansing
  • One wet wipe for rinsing
  • One sterile collection container (specimen cup)
  1. Wash your hands well with soap and water.
  2. Place the supplies so they are within reach of the toilet.
  3. Remove the lid from the specimen cup and place it flat side down. Make sure you don't touch the inside of the lid or cup.
  4. Prepare to use the bathroom as you normally would. If you are uncircumcised, pull back your foreskin. Take a soapy wipe and clean the head of your penis by making a stroke around the end of the penis toward your stomach. Discard the wipe.
  5. Repeat this procedure with each of the other two soapy wipes, discarding each after one use.
  6. Repeat using the wet wipe to rinse the penis.
  7. Urinate a small amount into the toilet or urinal, then stop the flow. Hold the specimen cup a few inches away from your penis and urinate into the cup until it is a third full. Don't let the cup overflow. Remove the cup from the stream and finish urinating into the toilet.
  8. Place the lid tightly on the cup, being careful not to touch the inside of the cup or lid with your fingers. If uncircumcised, be sure to pull the foreskin forward again.
  9. Wash your hands and leave the cup in the bathroom.

Last Updated 09/2012