Analyzers
The RFCC at Cincinnati Children’s maintains seven analytical cytometers for measuring fluorescence in cellular applications including immunofluorescence, cell cycle analysis, proliferation and phospho-flow. The instruments are available 24/7 for those who have been trained by our staff.
| | | | Number of Fluorescence PMTs |
| Instrument Name on Equipment Booking System* | Instrument Make/Model** | Location*** | Blue (488nm) | Red (635nm) | Violet (405nm) | Yellow-Green (561nm) | UV (335nm) |
| FACSCalibur I | BD FACSCalibur | R5524 | 3 | 1 | | | |
| FACSCalibur II | BD FACSCalibur | R5524 | 3 | 1 | | | |
| FACSCanto I | BD FACSCanto | R5087A | 2 | 3 | | 4 | |
| FACSCanto II | BD FACSCanto II | R5087A | 4 | 2 | 2 | | |
| FACSCanto III | BD FACSCanto | R5087A | 5 | 3 | 2 | | |
| LSR II | BD LSR II | R5524 | 2 | 3 | 2 | 5 | 2 |
| LSRFortessa | BD LSRFortessa | R5524 | 5 | 3 | 2 | | |
Analyzer Information |
Location
R5524: FACSCalibur's, LSR's
R5087A: FACSCanto's Hours
24/7 for those who have been trained by our staff Contact Us
General information, help and analyzer training:
513-636-3575
Analyzer rooms:
513-636-3332 To Schedule Time
Research Equipment Booking System |
 |
*Click on the instrument name for the configuration.
**Only the following BD Falcon tubes can be used on our instruments. BD Cat. No. 352058, 352052, 352008, 352235. Any other tube will damage the instrument!
***BL2 rooms. Doors must be kept closed at all times and appropriate PPE worn while in the instrument rooms.
Scheduling
After training, you can schedule time using the Research Equipment Booking System. Times of more than four hours must be approved by flow core staff. Schedule only for the time you need and delete any unused time.
Fees
Cost for using the analyzers is $37 / hour rounded up to the nearest half hour. Digestive Health Center members receive a discount. See the DHC website for more details.
Technical Support
Need help? We can be reached in R5543, R5564 or at 513-636-3575.
Data Storage
A project drive has been set up called “RFCC-FACSDATA.” Directions on how to access the project drive will be provided at training or refer to our data management policy for further information.
Request access to one of our project drives at https://bmirdsdp.cchmc.org/DAP/
Data Analysis
Any time spent on the acquisition computers is considered billable time. However, computers with FACSDiva, CellQuestPro, FlowJo, FCS Express, and ModFit LT are available for data analysis free of charge to flow core users in R5543. See "Software" for more information.
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