Analytical Cytometers for Measuring Fluorescence in Cellular Applications
The Research Flow Cytometry Facility at Cincinnati Children's maintains ten analytical cytometers for measuring fluorescence in cellular applications including immunofluorescence, cell cycle analysis, proliferation and phospho-flow.
Design a panel on one of our instruments using FluoroFinder.
All our analyzers are located in R1.025. In this BL2 room, doors must be kept closed at all times and appropriate PPE worn while in the instrument rooms.
| Instrument Name* | Instrument Make/Model** | Plate loader | UV (355nm) | Violet (405nm) | Blue (488nm) | Yellow-Green (561nm) | Red (635nm) |
| Fortessa 1 | BD/LSRFortessa | 2 | 6 |
2 |
5 | 3 |
|
| Fortessa 2 | BD/LSRFortessa | 2 | 6 |
2 |
5 | 3 |
|
| Fortessa 3 |
BD/LSRFortessa | 2 | 6 |
2 |
5 | 3 |
|
| Mike (A5 SE 1) |
BD/FACSymphony A5 SE | 10 |
14 |
9 |
9 |
6 |
|
| Sully (A5 SE 2) |
BD/FACSymphony A5 SE |
10 |
14 |
9 |
9 |
6 |
|
| Aurora | Cytek/Aurora | Yes | 16 |
16 |
14 |
10 |
8 |
| Maleficent | Cytek/Aurora | Yes | 16 |
16 |
14 |
10 |
8 |
| Diablo | Cytek/Northern Lights | 16 | 14 | 8 | |||
*Click on the instrument name for the configuration.
**Only the following BD Falcon tubes can be used on our instruments. BD Cat. No. 352058, 352052, 352008, 352235. Any other tube will damage the instrument.
Sample Transportation to the RFCF
All samples must be placed in sealed capped tubes and in a secondary leak-proof container with absorbent. Please refer to the Transporting Samples to the RFCF document.
Technical Support
This video provides insight on how to import/export templates on FACSDiva.
Please look at this troubleshooting document for basic troubleshooting of the BD instruments.
Look at those resources for more information on Spectral Flow Cytometry.
- Cytek resources
- Panel design: SpectroLearn with troubleshooting advice
- Overview of the software SpectroFlo
- Create and unmix an experiment in SpectroFlo
- Unmixing best practices
- Worksheet tool
- Batch analysis
- Area Scaling
- University of Chicago resources
Need help? Contact us.
Data Storage
A project drive has been set up called “RFCC-FACSDATA.” For the Aurora, the drive is called "RFCC-AURORA." Directions on how to access the project drive will be provided at training or refer to our data management policy for further information.
Cincinnati Children's users only: Request access to one of our project drives.
Data Analysis
Any time spent on the acquisition computers is considered billable time. However, computers are available for data analysis free of charge to flow core users in R1.027. See "Software" for more information.
Cancellation Policies and Fees
You need to cancel your appointment via Stratocore. All cancellations will be confirmed via email to the client. Contact us for more information about our policies and fees.
Subscribe to Our Email List
Subscribe to the ResearchFlowCore email list by completing this online form to receive core updates and announcements.
Location and Contact Information
Location: Burnet Campus, R Building, 1st Floor, R1.028.
Hours: 24/7 for those who have been trained by our staff
Contact, Fees and Scheduling: For more information about our equipment, services, training and pricing or to begin working with us, please contact us. Reference our billing policies for details about other fees.
