Research Flow Cytometry Core

  • Analyzers

    The RFCC at Cincinnati Children’s maintains seven analytical cytometers for measuring fluorescence in cellular applications including immunofluorescence, cell cycle analysis, proliferation and phospho-flow. The instruments are available 24/7 for those who have been trained by our staff.

    Number of Fluorescence PMTs
    Instrument Name on Equipment Booking System* Instrument Make/Model** Location*** Blue (488nm) Red (635nm) Violet (405nm) Yellow-Green (561nm) UV (335nm)
    Calibur 1 BD FACSCaliburR5524 3 1
    Calibur 2 BD FACSCalibur R5524 3 1
    Canto 1 BD FACSCanto R5087A 2 3 4
    Canto 2 BD FACSCanto II R5087A 4 2 2
    Canto 3 BD FACSCantoR5087A5 3 2
    LSR II BD LSR II R5524 2 3 2 5 2
    Fortessa 1 BD LSRFortessa R5524 5 3 2

    Analyzer Information

    Location
    R5524: FACSCalibur's, LSR's
    R5087A: FACSCanto's

    Hours
    24/7 for those who have been trained by our staff

    Contact Us
    General information, help and analyzer training:
    513-636-3575
    Analyzer rooms:
    513-636-3332

    To Schedule Time
    Research Equipment Booking System

    contact us image

    *Click on the instrument name for the configuration.
    **Only the following BD Falcon tubes can be used on our instruments. BD Cat. No. 352058, 352052, 352008, 352235. Any other tube will damage the instrument!
    ***BL2 rooms. Doors must be kept closed at all times and appropriate PPE worn while in the instrument rooms.

    Scheduling

    After training, you can schedule time using the Research Equipment Booking System. Times of more than four hours must be approved by flow core staff.  Schedule only for the time you need and delete any unused time.

    Fees

    Cost for using the analyzers is $37 / hour rounded up to the nearest half hour. Digestive Health Center members receive a discount. See the DHC website for more details.

    Technical Support

    Need help?  We can be reached in R5543, R5564 or at 513-636-3575.

    Data Storage

    A project drive has been set up called “RFCC-FACSDATA.” Directions on how to access the project drive will be provided at training or refer to our data management policy for further information.

    Request access to one of our project drives at https://bmirdsdp.cchmc.org/DAP/

    Data Analysis

    Any time spent on the acquisition computers is considered billable time. However, computers with FACSDiva, CellQuest Pro, FlowJo, FCS Express, and ModFit LT are available for data analysis free of charge to flow core users in R5543. See "Software" for more information.

    Subscribe to our email list

    Subscribe to the ResearchFlowCore email list by completing this online form to receive core updates and announcements.